Biosynthesis of selenium nanoparticles with orange peel waste in microwave: characterization, and spectral properties
6 affiliations
- 1Laboratory of Nanobiotechnology, Darcy Ribeiro University Campus, University of Brasília (UnB), Brasília, 70910-900 DF Brazil
- 2School of Health Sciences and Technologies, Campus Ceilândia, University of Brasília (UnB), Brasília, 72220-275 Brazil
- 3Brazilian Agricultural Research Corporation (EMBRAPA), Embrapa Agroenergy, Brasília, 70770- 901 DF Brazil
- 4Green Nanotechnology Group, University of Brasília (UnB), Brasília, 72220-900 DF Brazil
- 5Laboratory of Microscopy and Microanalysis, University of Brasília (UnB), Brasília, 70910-900 DF Brazil
- 6Laboratory of Nanomaterials and Nanotoxicology, Department of Science and Technology, Federal University of São Paulo, São José Dos Campos, SP Brazil
Abstract
The objective of this study was to reuse agroindustrial waste from orange peels to obtain an orange peel aqueous extract (OPEa), used in the biosynthesis and characterization of selenium nanoparticles (SeNPs). The OPEa acted as a reducing and stabilizing agent in the synthesis of SeNPs, due to its polyphenolic compounds, such as flavonoids, alkaloids, terpenoids, and coumarins, identified by UHPLC-HRMS/MS. The synthesis of SeNPs was conducted in a microwave reactor, at 120 °C and 150 °C, for 30 min, using different volumetric proportions/concentrations between OPEa and sodium selenite (Na₂SeO₃) (5 and 10 mM), in ratios of 1:4, 2:3, 1:1, 3:2, and 4:1 (OPEa(mL):Na2SeO3(mL)). The SeNPs were characterized by visual inspection, UV/Vis, DLS, pH, TEM, SEM, and EDX. The results showed that SeNPs were successfully biosynthesized under all conditions, exhibiting distinct characteristics depending on the synthesis conditions. The formation of SeNPs was confirmed by the chromatic transition from greenish tones to light yellow to intense red and by UV/Vis spectrometry, with absorption peaks in the range of 250–340 nm. The SeNPs exhibited a hydrodynamic diameter (HD) of 213.6–1296 nm, with mono- and polydispersity characteristics (PdI: 0.033–0.885) and Zeta potential (ZP) between − 24.2 to -54.9 mV. Among the conditions tested, SeNPs with the best characteristics presented more homogeneous UV/Vis bands, lower HD (273.2–282.8 nm), and PdI (0.089–0.231), high ZP (-34.4 to -36.5 mV), stable pH (6), clear, translucent, and pellet-free colloidal solution, spherical morphology, and selenium accounted for 20.17% of its elemental composition.
Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s13205-026-05115-0.
Related
- Gamma-irradiation applied to the synthesis of selenium nanoparticles and its influence on physicochemical properties
- Screening Endophytic Fungi of Australian Indigenous Plants for Biosynthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Properties
- Biosynthesis, characterization, and acaricidal activity of silver nanoparticles from Ficus carica leaf extract against Rhipicephalus (Boophilus) microplus ticks, a Vector of Bacterial Pathogens".
- Endophytic fungi-mediated biosynthesis of titanium dioxide nanoparticles: characterization and evaluation of their antimicrobial, antioxidant, and anticancer activities
- Cucurbit[7]uril-mediated aqueous self-assembly and supramolecular functionalisation of selenium nanoparticles
BibTeX
@article{oliveira2026biosynthesis,
title = {{Biosynthesis of selenium nanoparticles with orange peel waste in microwave: characterization, and spectral properties}},
author = {Tainá Pereira da Silva Oliveira and Alan Kelbis Oliveira Lima and Talita Pereira Gonçalves and Marcelo Henrique Sousa and José Antônio de Aquino Ribeiro and Clenilson Martins Rodrigues and Isadora Florêncio and Sônia Nair Báo and Dayane Batista Tada and Hugo de Campos Braga and Elton Clementino da Silva and Luis Alexandre Muehlmann},
journal = {3 Biotech},
year = {2026},
month = nov,
doi = {10.1007/s13205-026-05115-0},
url = {https://europepmc.org/article/PMC/PMC13728017},
note = {CC BY},
}
RIS
TY - JOUR
TI - Biosynthesis of selenium nanoparticles with orange peel waste in microwave: characterization, and spectral properties
AU - Tainá Pereira da Silva Oliveira
AU - Alan Kelbis Oliveira Lima
AU - Talita Pereira Gonçalves
AU - Marcelo Henrique Sousa
AU - José Antônio de Aquino Ribeiro
AU - Clenilson Martins Rodrigues
AU - Isadora Florêncio
AU - Sônia Nair Báo
AU - Dayane Batista Tada
AU - Hugo de Campos Braga
AU - Elton Clementino da Silva
AU - Luis Alexandre Muehlmann
PY - 2026
DA - 2026/11/01
JO - 3 Biotech
DO - 10.1007/s13205-026-05115-0
UR - https://europepmc.org/article/PMC/PMC13728017
AB - The objective of this study was to reuse agroindustrial waste from orange peels to obtain an orange peel aqueous extract (OPEa), used in the biosynthesis and characterization of selenium nanoparticles (SeNPs). The OPEa acted as a reducing and stabilizing agent in the synthesis of SeNPs, due to its polyphenolic compounds, such as flavonoids, alkaloids, terpenoids, and coumarins, identified by UHPLC-HRMS/MS. The synthesis of SeNPs was conducted in a microwave reactor, at 120 °C and 150 °C, for 30 min, using different volumetric proportions/concentrations between OPEa and sodium selenite (Na₂SeO₃) (5 and 10 mM), in ratios of 1:4, 2:3, 1:1, 3:2, and 4:1 (OPEa(mL):Na2SeO3(mL)). The SeNPs were characterized by visual inspection, UV/Vis, DLS, pH, TEM, SEM, and EDX. The results showed that SeNPs were successfully biosynthesized under all conditions, exhibiting distinct characteristics depending on the synthesis conditions. The formation of SeNPs was confirmed by the chromatic transition from greenish tones to light yellow to intense red and by UV/Vis spectrometry, with absorption peaks in the range of 250–340 nm. The SeNPs exhibited a hydrodynamic diameter (HD) of 213.6–1296 nm, with mono- and polydispersity characteristics (PdI: 0.033–0.885) and Zeta potential (ZP) between − 24.2 to -54.9 mV. Among the conditions tested, SeNPs with the best characteristics presented more homogeneous UV/Vis bands, lower HD (273.2–282.8 nm), and PdI (0.089–0.231), high ZP (-34.4 to -36.5 mV), stable pH (6), clear, translucent, and pellet-free colloidal solution, spherical morphology, and selenium accounted for 20.17% of its elemental composition. Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s13205-026-05115-0.
SN - 2190-572X
SN - 2190-5738
ER -