First Identification and Genomic Characterization of Mammalian Orthoreovirus Type 1 in a Heavily Polluted Stream from an Underserved Urban Settlement in Brazil.
5 affiliations
- 1Laboratory of Medical Investigation in Virology (LIM-52), Instituto de Medicina Tropical de São Paulo, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, São Paulo 05403-000, Brazil.
- 2Department of Infectology and Tropical Medicine, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, Brazil.
- 3Department of Environmental Health, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil.
- 4NARA-Center for Research in Environmental Risk Assessment, São Paulo 01246-904, Brazil.
- 5Department of Environmental Analysis, Division of Microbiology and Parasitology, Environmental Company of the State of São Paulo (CETESB), São Paulo 05459-900, Brazil.
Abstract
Mammalian orthoreovirus (MRV), a member of the family Spinareoviridae within the order Reovirales, has a ten-segmented double-stranded RNA genome and is known for its environmental stability and broad host range, including humans. Although human MRV infections are usually asymptomatic or mild, occasional severe MRV-associated disease has been reported. Despite the widespread presence of MRV, particularly in contaminated water, genomic data from South America are limited, especially from environmental sources in underserved urban settlements. In this study, we isolated a virus from a heavily polluted stream running through an underprivileged, underserved, urban settlement in São Paulo, Brazil. The causative agent was identified as a novel MRV strain (MRV1_SP/BR) by whole-genome Illumina sequencing, and its presence was subsequently confirmed by RT-qPCR. This analysis revealed a complex mosaic genome architecture indicative of genetic reassortment. Based on phylogenetic analysis of the S1 segment, the strain was genetically classified as MRV-1, whereas its remaining segments showed high nucleotide identity to sequences previously detected in humans and bats. These findings provide the first genomic characterization of a reassortant MRV detected in an urban stream in South America and expand the available information on MRV diversity in sewage-impacted aquatic environments.
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BibTeX
@article{paula2026first,
title = {{First Identification and Genomic Characterization of Mammalian Orthoreovirus Type 1 in a Heavily Polluted Stream from an Underserved Urban Settlement in Brazil.}},
author = {Anderson Vicente de Paula and Layla Honorato and Lucy Santos Vilas Boas and Anna Sara Shafferman Levin and Maria Tereza Pepe Razzolini and Ronalda Silva de Araújo and Mikaela Renata Funada Barbosa and Antonio Charlys da Costa and Maria Cassia Mendes-Correa},
journal = {Viruses},
year = {2026},
month = oct,
doi = {10.3390/v18101161},
url = {https://europepmc.org/article/MED/42944080},
note = {CC BY},
}
RIS
TY - JOUR
TI - First Identification and Genomic Characterization of Mammalian Orthoreovirus Type 1 in a Heavily Polluted Stream from an Underserved Urban Settlement in Brazil.
AU - Anderson Vicente de Paula
AU - Layla Honorato
AU - Lucy Santos Vilas Boas
AU - Anna Sara Shafferman Levin
AU - Maria Tereza Pepe Razzolini
AU - Ronalda Silva de Araújo
AU - Mikaela Renata Funada Barbosa
AU - Antonio Charlys da Costa
AU - Maria Cassia Mendes-Correa
PY - 2026
DA - 2026/10/19
JO - Viruses
DO - 10.3390/v18101161
UR - https://europepmc.org/article/MED/42944080
AB - Mammalian orthoreovirus (MRV), a member of the family Spinareoviridae within the order Reovirales, has a ten-segmented double-stranded RNA genome and is known for its environmental stability and broad host range, including humans. Although human MRV infections are usually asymptomatic or mild, occasional severe MRV-associated disease has been reported. Despite the widespread presence of MRV, particularly in contaminated water, genomic data from South America are limited, especially from environmental sources in underserved urban settlements. In this study, we isolated a virus from a heavily polluted stream running through an underprivileged, underserved, urban settlement in São Paulo, Brazil. The causative agent was identified as a novel MRV strain (MRV1_SP/BR) by whole-genome Illumina sequencing, and its presence was subsequently confirmed by RT-qPCR. This analysis revealed a complex mosaic genome architecture indicative of genetic reassortment. Based on phylogenetic analysis of the S1 segment, the strain was genetically classified as MRV-1, whereas its remaining segments showed high nucleotide identity to sequences previously detected in humans and bats. These findings provide the first genomic characterization of a reassortant MRV detected in an urban stream in South America and expand the available information on MRV diversity in sewage-impacted aquatic environments.
SN - 1999-4915
ER -