Novel Chitosan Bioink Derived from Eurycantha calcarata (Lucas, 1869) for 3D Bioprinting of Cell-Compatible Scaffolds
Abstract
Three-dimensional (3D) printing of eco-friendly, naturally derived biopolymers such as chitosan offers great potential for biomedical applications. In this work, Eurycantha calcarata insects were used as the source of chitosan with a yield of 22.2% and a deacetylation degree of 77-78%, comparable to that of commercial chitosan. After extraction, characterizations were performed on the obtained compound (including FTIR, NMR, SEM, TGA, and XRD). Formulated into chitosan:gelatin hydrogels (EC:G = 1:3, 1:4, 1:5), the blends exhibited solid-liquid transitions at 29-33 °C depending on gelatin content. 3D-printed scaffolds achieved >90% shape accuracy and remained stable for 21 days in a culture medium. HeLa cells cultured on the scaffolds showed up to 99.6% viability after 3 days, demonstrating excellent cytocompatibility. By valorizing insect-derived waste, this work presents a sustainable and high-performance chitosan bioink, addressing the growing demand for eco-friendly materials in 3D bioprinting.
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BibTeX
@article{mei2026novel,
title = {{Novel Chitosan Bioink Derived from Eurycantha calcarata (Lucas, 1869) for 3D Bioprinting of Cell-Compatible Scaffolds}},
author = {Zhenying Mei and Julie Lagouarde and Olga Volkova and Virginie Baylot and Christophe Candet and Philippe Barthélémy and Pavel Kuzhir and Guilhem Godeau},
journal = {ACS Omega},
year = {2026},
month = sep,
doi = {10.1021/acsomega.5c10302},
url = {https://hal.science/hal-05765476v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Novel Chitosan Bioink Derived from Eurycantha calcarata (Lucas, 1869) for 3D Bioprinting of Cell-Compatible Scaffolds
AU - Zhenying Mei
AU - Julie Lagouarde
AU - Olga Volkova
AU - Virginie Baylot
AU - Christophe Candet
AU - Philippe Barthélémy
AU - Pavel Kuzhir
AU - Guilhem Godeau
PY - 2026
DA - 2026/09/26
JO - ACS Omega
DO - 10.1021/acsomega.5c10302
UR - https://hal.science/hal-05765476v1
AB - Three-dimensional (3D) printing of eco-friendly, naturally derived biopolymers such as chitosan offers great potential for biomedical applications. In this work, Eurycantha calcarata insects were used as the source of chitosan with a yield of 22.2% and a deacetylation degree of 77-78%, comparable to that of commercial chitosan. After extraction, characterizations were performed on the obtained compound (including FTIR, NMR, SEM, TGA, and XRD). Formulated into chitosan:gelatin hydrogels (EC:G = 1:3, 1:4, 1:5), the blends exhibited solid-liquid transitions at 29-33 °C depending on gelatin content. 3D-printed scaffolds achieved >90% shape accuracy and remained stable for 21 days in a culture medium. HeLa cells cultured on the scaffolds showed up to 99.6% viability after 3 days, demonstrating excellent cytocompatibility. By valorizing insect-derived waste, this work presents a sustainable and high-performance chitosan bioink, addressing the growing demand for eco-friendly materials in 3D bioprinting.
SN - 2470-1343
ER -