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Peer-reviewedCC BYScientific Reports

Temperature drives microbial community shifts and methanogenic pathways in a carbon fiber fixed-bed reactor for anaerobic digestion

  • Xiaoli Pan1
  • Hong Li2
  • Jiemin Zhu3
  • Xugen Chen4
  • Yongjiang Zhang1
  • Jie Hu1
  • Lijun Wu1
  • Xixi Li1
  • Yuhua Han1
  • Feng Luo3
4 affiliations
  1. 1Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College
  2. 2Key Laboratory for Brewing Microorganism Application and Detection Technology, Luzhou Vocational and Technical College
  3. 3Chongqing Key Laboratory of Bioresource for Bioenergy, College of Resources and Environment, Southwest University
  4. 4Chemical Center, Chongqing Shiyi Product Quality Testing Co., Ltd
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Abstract

This study investigated the effects of temperature on microbial communities, key enzymes, and energy metabolism in carbon fiber fixed-bed anaerobic reactors under psychrophilic (15 °C), mesophilic (37 °C), and thermophilic (55 °C) conditions. Metagenomic and 16S rRNA gene sequencing revealed that methane content peaked at 80% under psychrophilic conditions, despite lower biogas production (7.5 L/d) compared to mesophilic (25 L/d) and thermophilic (30 L/d) regimes. Bacterial diversity was highest at 15 °C, while archaeal richness peaked at 37 °C. Methanothermobacter and Methanosarcina dominated thermophilic methanogenesis, whereas Methanothrix prevailed at lower temperatures. Genes encoding F420-reducing hydrogenase were most abundant at 55 °C, where biogas production was highest, while CO2-reducing methanogenesis genes were well represented at 15 °C, where methane purity reached 80%. ATP synthesis genes shifted from bacteria-associated (37 °C) to archaea-associated (55 °C) dominance. These findings highlight the genetic potential of psychrophilic systems for achieving high methane purity and provide insights for optimizing anaerobic digestion across temperature gradients.

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Cite

BibTeXRIS
BibTeX
@article{pan2026temperature,
  title = {{Temperature drives microbial community shifts and methanogenic pathways in a carbon fiber fixed-bed reactor for anaerobic digestion}},
  author = {Xiaoli Pan and Hong Li and Jiemin Zhu and Xugen Chen and Yongjiang Zhang and Jie Hu and Lijun Wu and Xixi Li and Yuhua Han and Feng Luo},
  journal = {Scientific Reports},
  year = {2026},
  month = jul,
  doi = {10.1038/s41598-026-61884-x},
  url = {https://doi.org/10.1038/s41598-026-61884-x},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Temperature drives microbial community shifts and methanogenic pathways in a carbon fiber fixed-bed reactor for anaerobic digestion
AU  - Xiaoli Pan
AU  - Hong Li
AU  - Jiemin Zhu
AU  - Xugen Chen
AU  - Yongjiang Zhang
AU  - Jie Hu
AU  - Lijun Wu
AU  - Xixi Li
AU  - Yuhua Han
AU  - Feng Luo
PY  - 2026
DA  - 2026/07/01
JO  - Scientific Reports
DO  - 10.1038/s41598-026-61884-x
UR  - https://doi.org/10.1038/s41598-026-61884-x
AB  - This study investigated the effects of temperature on microbial communities, key enzymes, and energy metabolism in carbon fiber fixed-bed anaerobic reactors under psychrophilic (15 °C), mesophilic (37 °C), and thermophilic (55 °C) conditions. Metagenomic and 16S rRNA gene sequencing revealed that methane content peaked at 80% under psychrophilic conditions, despite lower biogas production (7.5 L/d) compared to mesophilic (25 L/d) and thermophilic (30 L/d) regimes. Bacterial diversity was highest at 15 °C, while archaeal richness peaked at 37 °C. Methanothermobacter and Methanosarcina dominated thermophilic methanogenesis, whereas Methanothrix prevailed at lower temperatures. Genes encoding F420-reducing hydrogenase were most abundant at 55 °C, where biogas production was highest, while CO2-reducing methanogenesis genes were well represented at 15 °C, where methane purity reached 80%. ATP synthesis genes shifted from bacteria-associated (37 °C) to archaea-associated (55 °C) dominance. These findings highlight the genetic potential of psychrophilic systems for achieving high methane purity and provide insights for optimizing anaerobic digestion across temperature gradients.
SN  - 2045-2322
ER  -