Shape-Dependent Interactions of Gold Nanoparticles with Microalgae: Distinct Cellular and Molecular Responses.
- 1Department of Chemical and Environmental Engineering, University of California Riverside, Riverside, California 92521, United States.
- 2Department of Mechanical and Aerospace Engineering, University at Buffalo, Buffalo, New York 14260, United States.
- 3Department of Botany and Plant Sciences, University of California Riverside, Riverside, California 92521, United States.
Abstract
The ecotoxicological effects of engineered nanoparticles in aquatic environments are influenced not only by composition and size but also by particle morphology, yet shape-dependent interactions with primary producers remain poorly understood. In this study, we evaluated the cellular and molecular responses of freshwater algae (Chlamydomonas reinhardtii) following exposure to 100 nm silica-coated gold nanospheres (AuNPs) and nanostars (AuNSs) across multiple concentrations. Exposure to 10 mg/L AuNSs for 72 h results in significantly stronger inhibition of algal growth and photosynthetic activity compared to the same concentration of AuNPs. Morphometric profiling reveals that AuNSs induce pronounced structural injury, including cell enlargement, debris production, and disruption of subcellular organization, more than AuNPs. Confocal imaging suggested that this heightened toxicity may stem from two distinct internalization patterns: AuNPs primarily adhered to chloroplast surfaces, whereas AuNSs caused more extensive membrane disruption and were observed deeper within intracellular compartments. RNA sequencing identified 9 upregulated and 38 downregulated differentially expressed genes (DEGs) in the 10 mg/L AuNP-treated cells, impairing photosynthesis and energy storage via the photosystem II subunit S1 (PSBS1)/early light-inducible protein (ELI3) pathway. In contrast, the AuNS group exhibits 246 upregulated and 145 downregulated DEGs, affecting membrane integrity and nitrogen metabolism through the nitrate reductase (NIT1)/aminomethyl transferase (AMT1)/protein kinase domain-containing protein (A0A2K3CRU5) pathway. These results demonstrate that the nanoparticle morphology can drive divergent toxicity mechanisms in algal cells. Our findings highlight the necessity of incorporating NP morphology into environmental risk assessments and suggest that safer nanomaterial design should consider shape-dependent interactions with aquatic microorganisms.
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BibTeX
@article{wang2026shapedependent,
title = {{Shape-Dependent Interactions of Gold Nanoparticles with Microalgae: Distinct Cellular and Molecular Responses.}},
author = {Can Wang and Bhaskar Sharma and Ruonan Peng and Xin Yong and Louis S Santiago and Ke Du},
journal = {Environment \& health (Washington, D.C.)},
year = {2026},
month = jul,
doi = {10.1021/envhealth.5c00839},
url = {https://europepmc.org/article/MED/42903400},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Shape-Dependent Interactions of Gold Nanoparticles with Microalgae: Distinct Cellular and Molecular Responses.
AU - Can Wang
AU - Bhaskar Sharma
AU - Ruonan Peng
AU - Xin Yong
AU - Louis S Santiago
AU - Ke Du
PY - 2026
DA - 2026/07/15
JO - Environment & health (Washington, D.C.)
DO - 10.1021/envhealth.5c00839
UR - https://europepmc.org/article/MED/42903400
AB - The ecotoxicological effects of engineered nanoparticles in aquatic environments are influenced not only by composition and size but also by particle morphology, yet shape-dependent interactions with primary producers remain poorly understood. In this study, we evaluated the cellular and molecular responses of freshwater algae (Chlamydomonas reinhardtii) following exposure to 100 nm silica-coated gold nanospheres (AuNPs) and nanostars (AuNSs) across multiple concentrations. Exposure to 10 mg/L AuNSs for 72 h results in significantly stronger inhibition of algal growth and photosynthetic activity compared to the same concentration of AuNPs. Morphometric profiling reveals that AuNSs induce pronounced structural injury, including cell enlargement, debris production, and disruption of subcellular organization, more than AuNPs. Confocal imaging suggested that this heightened toxicity may stem from two distinct internalization patterns: AuNPs primarily adhered to chloroplast surfaces, whereas AuNSs caused more extensive membrane disruption and were observed deeper within intracellular compartments. RNA sequencing identified 9 upregulated and 38 downregulated differentially expressed genes (DEGs) in the 10 mg/L AuNP-treated cells, impairing photosynthesis and energy storage via the photosystem II subunit S1 (PSBS1)/early light-inducible protein (ELI3) pathway. In contrast, the AuNS group exhibits 246 upregulated and 145 downregulated DEGs, affecting membrane integrity and nitrogen metabolism through the nitrate reductase (NIT1)/aminomethyl transferase (AMT1)/protein kinase domain-containing protein (A0A2K3CRU5) pathway. These results demonstrate that the nanoparticle morphology can drive divergent toxicity mechanisms in algal cells. Our findings highlight the necessity of incorporating NP morphology into environmental risk assessments and suggest that safer nanomaterial design should consider shape-dependent interactions with aquatic microorganisms.
SN - 2833-8278
ER -