γ-Linolenic Acid Inhibits Contractile Responses in Pig Basilar Arteries via Prostanoid TP Receptor Antagonism and Ba²⁺-Sensitive K⁺ Channel Activation
Abstract
γ-Linolenic acid (GLA) is an n-6 polyunsaturated fatty acid (PUFA) with reported vasoprotective effects. GLA has been shown to inhibit contractions in pig coronary arteries via competitive antagonism at prostanoid TP receptors. α-Linolenic acid (ALA), an n-3 PUFA and a structural isomer of GLA, has been shown to inhibit contractions in pig basilar arteries (PBAs) via prostanoid TP receptor antagonism and activation of K+ channels. However, the actions of GLA in cerebral arteries remain unclear. In this study, we examined the effects of GLA on contractile responses in PBAs. GLA (10−5–10−4 M) concentration-dependently inhibited contractions induced by the TP receptor agonist U46619 (3 × 10−8 M) and prostaglandin F2α (PGF2α; 3 × 10−6 M). GLA (3 × 10−6–3 × 10−5 M) shifted the concentration–response curve for U46619 to the right. Schild analysis yielded a slope of 1.33 (95% confidence interval: 0.70–1.96), which was not significantly different from unity, but GLA also reduced the maximal response to U46619. The apparent pA2 value was 5.43. GLA (10−4 M) also inhibited PGF2α-induced contractions in the presence of the TP receptor antagonist SQ 29,548 (10−6 M); this inhibitory effect was attenuated by Ba2+ (10−3 M) but not by other tested K+ channel inhibitors. GLA also inhibited endothelin-1 (10−8–3 × 10−8 M)-induced contractions, and this effect was attenuated by Ba2+. These findings indicate that GLA, like ALA, suppresses contractile responses in PBAs through dual mechanisms involving TP receptor antagonism and activation of Ba2+-sensitive K+ channels.
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BibTeX
@article{yoshioka2026γlinolenic,
title = {{γ-Linolenic Acid Inhibits Contractile Responses in Pig Basilar Arteries via Prostanoid TP Receptor Antagonism and Ba²⁺-Sensitive K⁺ Channel Activation}},
author = {Kento Yoshioka and Minami Inagaki and Tam Minh Phi and Haruki Kimura and Keigo Osa and Keisuke Obara and Noritaka Nakamichi and Yoshio Tanaka},
journal = {Biological and Pharmaceutical Bulletin},
year = {2026},
month = sep,
doi = {10.1248/bpb.b26-00185},
url = {https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00185/_article},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - γ-Linolenic Acid Inhibits Contractile Responses in Pig Basilar Arteries via Prostanoid TP Receptor Antagonism and Ba²⁺-Sensitive K⁺ Channel Activation
AU - Kento Yoshioka
AU - Minami Inagaki
AU - Tam Minh Phi
AU - Haruki Kimura
AU - Keigo Osa
AU - Keisuke Obara
AU - Noritaka Nakamichi
AU - Yoshio Tanaka
PY - 2026
DA - 2026/09/04
JO - Biological and Pharmaceutical Bulletin
DO - 10.1248/bpb.b26-00185
UR - https://www.jstage.jst.go.jp/article/bpb/49/9/49_b26-00185/_article
AB - γ-Linolenic acid (GLA) is an n-6 polyunsaturated fatty acid (PUFA) with reported vasoprotective effects. GLA has been shown to inhibit contractions in pig coronary arteries via competitive antagonism at prostanoid TP receptors. α-Linolenic acid (ALA), an n-3 PUFA and a structural isomer of GLA, has been shown to inhibit contractions in pig basilar arteries (PBAs) via prostanoid TP receptor antagonism and activation of K+ channels. However, the actions of GLA in cerebral arteries remain unclear. In this study, we examined the effects of GLA on contractile responses in PBAs. GLA (10−5–10−4 M) concentration-dependently inhibited contractions induced by the TP receptor agonist U46619 (3 × 10−8 M) and prostaglandin F2α (PGF2α; 3 × 10−6 M). GLA (3 × 10−6–3 × 10−5 M) shifted the concentration–response curve for U46619 to the right. Schild analysis yielded a slope of 1.33 (95% confidence interval: 0.70–1.96), which was not significantly different from unity, but GLA also reduced the maximal response to U46619. The apparent pA2 value was 5.43. GLA (10−4 M) also inhibited PGF2α-induced contractions in the presence of the TP receptor antagonist SQ 29,548 (10−6 M); this inhibitory effect was attenuated by Ba2+ (10−3 M) but not by other tested K+ channel inhibitors. GLA also inhibited endothelin-1 (10−8–3 × 10−8 M)-induced contractions, and this effect was attenuated by Ba2+. These findings indicate that GLA, like ALA, suppresses contractile responses in PBAs through dual mechanisms involving TP receptor antagonism and activation of Ba2+-sensitive K+ channels.
SN - 1347-5215
SN - 0918-6158
ER -