Biological Properties of Promising Poly(isobutyl cyanoacrylate) Nanoparticles Coated with Fucoidan, Levan, or Chitosan
6 affiliations
- 1Instituto Keizo-Asami (iLIKA), Universidade Federal de Pernambuco (UFPE), Recife 50670-901, Brazil.
- 2Laboratório de Nanotecnologia, Biotecnologia e Cultura de Células, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco (CAV/UFPE), Recife 55608-680, Brazil.
- 3Faculté de Pharmacie Institut Galien Paris-Saclay - UMR-CNRS 8612, Université Paris-Saclay, Orsay 91400, France.
- 4Laboratório de Avanços em Biotecnologia de Proteínas e Tecnologia de Bioativos, Universidade Federal Rural de Pernambuco (UFRPE), Recife 52171-900, Brazil.
- 5Laboratório Integrado de Biotecnología Aplicada à Saúde, Universidade de Pernambuco (UPE), Recife 52171-900, Brazil.
- 6Laboratório de Análise Fitoquímica de Plantas Medicinais II, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza 60020-181, Brazil.
Abstract
The objective of this study was to comparatively evaluate the biological behavior of poly(isobutyl cyanoacrylate) nanoparticles coated with fucoidan, chitosan, or levan. We evaluated their in vitro hemocompatibility, coagulation profile, platelet aggregation, cytotoxicity, in vitro wound-healing activity, and stability during simulated gastrointestinal digestion. The three formulations exhibited distinct biological responses depending on the polysaccharide used. All nanosystems showed nonhemolytic action under the tested conditions. In coagulation and platelet assays, Chi-NPs displayed procoagulant tendencies and Lev-NPs also promoted coagulation, whereas Fuc-NPs demonstrated concentration-dependent anticoagulant effects. Cytotoxicity results varied across the assays, with the MTT assay showing cytotoxicity and apparent preservation of cell morphology, whereas the nanoparticles were not found to be cytotoxic using the flow cytometry technique. All formulations supported fibroblast migration in the wound-healing model, with Fuc-NPs showing the most pronounced effect. During simulated digestion, only Chi-NPs exhibited marked instability, with substantial size variation (123.2 ± 2.18 nm to 653.9 ± 534.41 nm) upon exposure to digestive enzymes. These findings highlight the importance of in vitro characterization as an initial step toward understanding how formulation-specific physicochemical properties influence biological behavior. However, the results are not sufficient to establish safety or suitability for in vivo administration. The differences observed among the formulations reflect not only the chemical identity of the polysaccharides but also the intrinsic physicochemical characteristics of each nanoparticle. Thus, this study should be interpreted as a comparative characterization of three distinct PIBCA systems.
Related
- Copper Nanoparticles at Optimized Concentrations Exhibit Minimal Intrinsic Biological Effects in Hepatocellular Carcinoma Cells
- Phytochemical profiling-guided green synthesis and characterisation of silver nanoparticles using Catunaregam spinosa for biological applications
- Extravascular Cyanoacrylate “Embolization” for the Treatment of Vesico-Vaginal Fistula: A Minimally Invasive Alternative
- Unexpected para-aortic venous migration of n-butyl cyanoacrylate during transarterial sac embolization for a type II endoleak: A technical pitfall.
- Design, Synthesis, and Biological Evaluation of Molecular Hybrids Incorporating Coumarin-Pyrazole-Linked Indolinone Derivatives With Antioxidant and Anti-Alzheimer Properties.
BibTeX
@article{cavalcanti2026biological,
title = {{Biological Properties of Promising Poly(isobutyl cyanoacrylate) Nanoparticles Coated with Fucoidan, Levan, or Chitosan}},
author = {Iago Dillion Lima Cavalcanti and Andreza Tallyne de Aguiar Silva and Leandro Paes de Brito and Clarice Beatriz Gonçalves Silva and Fabrício Oliveira Souto and Romero Marcos Pedrosa Brandão Costa and Otília Deusdênia Loiola Pessoa and Eloisa Berbel Manaia and Gilles Ponchel and Nereide Stela Santos Magalhães and Mariane Cajubá de Britto Lira Nogueira},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c08464},
url = {https://pubs.acs.org/acsodf/article/11/41/63893/5447844/Biological-Properties-of-Promising-Poly-isobutyl},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Biological Properties of Promising Poly(isobutyl cyanoacrylate) Nanoparticles Coated with Fucoidan, Levan, or Chitosan
AU - Iago Dillion Lima Cavalcanti
AU - Andreza Tallyne de Aguiar Silva
AU - Leandro Paes de Brito
AU - Clarice Beatriz Gonçalves Silva
AU - Fabrício Oliveira Souto
AU - Romero Marcos Pedrosa Brandão Costa
AU - Otília Deusdênia Loiola Pessoa
AU - Eloisa Berbel Manaia
AU - Gilles Ponchel
AU - Nereide Stela Santos Magalhães
AU - Mariane Cajubá de Britto Lira Nogueira
PY - 2026
DA - 2026/10/09
JO - ACS Omega
DO - 10.1021/acsomega.6c08464
UR - https://pubs.acs.org/acsodf/article/11/41/63893/5447844/Biological-Properties-of-Promising-Poly-isobutyl
AB - The objective of this study was to comparatively evaluate the biological behavior of poly(isobutyl cyanoacrylate) nanoparticles coated with fucoidan, chitosan, or levan. We evaluated their in vitro hemocompatibility, coagulation profile, platelet aggregation, cytotoxicity, in vitro wound-healing activity, and stability during simulated gastrointestinal digestion. The three formulations exhibited distinct biological responses depending on the polysaccharide used. All nanosystems showed nonhemolytic action under the tested conditions. In coagulation and platelet assays, Chi-NPs displayed procoagulant tendencies and Lev-NPs also promoted coagulation, whereas Fuc-NPs demonstrated concentration-dependent anticoagulant effects. Cytotoxicity results varied across the assays, with the MTT assay showing cytotoxicity and apparent preservation of cell morphology, whereas the nanoparticles were not found to be cytotoxic using the flow cytometry technique. All formulations supported fibroblast migration in the wound-healing model, with Fuc-NPs showing the most pronounced effect. During simulated digestion, only Chi-NPs exhibited marked instability, with substantial size variation (123.2 ± 2.18 nm to 653.9 ± 534.41 nm) upon exposure to digestive enzymes. These findings highlight the importance of in vitro characterization as an initial step toward understanding how formulation-specific physicochemical properties influence biological behavior. However, the results are not sufficient to establish safety or suitability for in vivo administration. The differences observed among the formulations reflect not only the chemical identity of the polysaccharides but also the intrinsic physicochemical characteristics of each nanoparticle. Thus, this study should be interpreted as a comparative characterization of three distinct PIBCA systems.
SN - 2470-1343
ER -