Placental LBX1 dysregulation drives ribosomal dysfunction and unfolded protein response in spontaneous…

Abstract

Spontaneous preterm birth (sPTB) affects [~]9.9% of pregnancies worldwide and is driven in part by placental dysfunction, yet the signalling events linking placental dysregulation to spontaneous prematurity remain incompletely defined. Using a cross-sectional design, we profiled 70 placentae from spontaneous term and preterm birth delivery to construct protein co-expression networks and identified 13 hub proteins associated with sPTB. Quantification of placental circulatory proteins in an independent nested case-control set of 53 maternal plasma samples highlighted elevated levels of the transcription factor ladybird homeobox protein 1 (LBX1) in maternal circulation from sPTB cases. Elevated LBX1 in trophoblast cells undergo KPNB1 mediated nuclear import, where it deregulates ribosomal biogenesis and perturbs subsequent ribosomal protein homeostasis, contributing to the accumulation of newly synthesised proteins. Accumulation of these nascent proteins triggers unfolded protein response which may lead to adverse pregnancy outcome. These findings implicate LBX1-mediated ribosomal disruption and activation of unfolded protein response in trophoblasts as a placental mechanism contributing to sPTB.

Graphical Abstract

O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=113 SRC="FIGDIR/small/26362823v1_ufig1.gif" ALT="Figure 1"> View larger version (56K): [email protected]@1801e5forg.highwire.dtl.DTLVardef@4a1bdaorg.highwire.dtl.DTLVardef@16a93b8_HPS_FORMAT_FIGEXP M_FIG C_FIG

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BibTeX

@misc{biswas2026placental,
  title = {{Placental LBX1 dysregulation drives ribosomal dysfunction and unfolded protein response in spontaneous preterm birth}},
  author = {A. Biswas and N. Kharbanda and K. S. Bisht and P. Tailor and S. Saha and A. Tiwari and M. Sharma and R. Thiruvengadam and N. Wadhwa and D. M. Salunke and S. Bhatnagar and G.-I. Study team and P. Kshetrapal and T. K. Maiti},
  howpublished = {medRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.11.26362823},
  url = {https://www.medrxiv.org/content/10.64898/2026.09.11.26362823v1},
  note = {CC BY-NC-ND},
}

RIS

TY  - UNPB
TI  - Placental LBX1 dysregulation drives ribosomal dysfunction and unfolded protein response in spontaneous preterm birth
AU  - A. Biswas
AU  - N. Kharbanda
AU  - K. S. Bisht
AU  - P. Tailor
AU  - S. Saha
AU  - A. Tiwari
AU  - M. Sharma
AU  - R. Thiruvengadam
AU  - N. Wadhwa
AU  - D. M. Salunke
AU  - S. Bhatnagar
AU  - G.-I. Study team
AU  - P. Kshetrapal
AU  - T. K. Maiti
PY  - 2026
DA  - 2026/09/11
PB  - medRxiv
DO  - 10.64898/2026.09.11.26362823
UR  - https://www.medrxiv.org/content/10.64898/2026.09.11.26362823v1
AB  - Spontaneous preterm birth (sPTB) affects [~]9.9% of pregnancies worldwide and is driven in part by placental dysfunction, yet the signalling events linking placental dysregulation to spontaneous prematurity remain incompletely defined. Using a cross-sectional design, we profiled 70 placentae from spontaneous term and preterm birth delivery to construct protein co-expression networks and identified 13 hub proteins associated with sPTB. Quantification of placental circulatory proteins in an independent nested case-control set of 53 maternal plasma samples highlighted elevated levels of the transcription factor ladybird homeobox protein 1 (LBX1) in maternal circulation from sPTB cases. Elevated LBX1 in trophoblast cells undergo KPNB1 mediated nuclear import, where it deregulates ribosomal biogenesis and perturbs subsequent ribosomal protein homeostasis, contributing to the accumulation of newly synthesised proteins. Accumulation of these nascent proteins triggers unfolded protein response which may lead to adverse pregnancy outcome. These findings implicate LBX1-mediated ribosomal disruption and activation of unfolded protein response in trophoblasts as a placental mechanism contributing to sPTB.  Graphical Abstract  O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=113 SRC="FIGDIR/small/26362823v1_ufig1.gif" ALT="Figure 1"> View larger version (56K): [email protected]@1801e5forg.highwire.dtl.DTLVardef@4a1bdaorg.highwire.dtl.DTLVardef@16a93b8_HPS_FORMAT_FIGEXP M_FIG C_FIG
ER  -

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