Comparison of Albino and Pigmented Rat Oxygen-Induced Retinopathy Models: From Vascular to Neural Retinal Changes.
5 affiliations
- 1INSERM, UMRS1138, Team 17, Physiopathology of Ocular Diseases: Therapeutic Innovations, Sorbonne University, Paris Cité University, Centre de Recherche des Cordeliers, Paris, France.
- 2Ophthalmology Department, Necker-Enfants Malades University Hospital, Assistance Publique Hôpitaux de Paris, Paris Cité University, Paris, France.
- 3Inovarion, Paris, France.
- 4Ophtalmopole, Cochin University Hospital, AP-HP, Assistance Publique Hôpitaux de Paris, Paris, France.
- 5Neonatology Department, Necker-Enfants Malades University Hospital, Assistance Publique Hôpitaux de Paris, Paris Cité University, Paris, France.
Abstract
Purpose
To compare two commonly used rat models of oxygen-induced retinopathy (OIR)-the 50/10 model in Sprague-Dawley rats and the 80/40 model in Long-Evans rats-to identify which more closely reproduces the features of human retinopathy.Methods
Dams and pups were exposed from postnatal day 0 (P0) to P14 to alternating oxygen cycles, followed by analyses at P14, P18, and P28. The 50/10 protocol alternated 50% and 10% oxygen every 24 hours, whereas the 80/40 protocol alternated 80% and 40% oxygen every 12 hours. The vascular, structural, and functional retinal changes were assessed using in vivo imaging and electrophysiology, as well as ex vivo histological and molecular analyses.Results
Both OIR protocols induced significant retinal vascular abnormalities compared with controls, but with marked differences in onset, severity, and persistence. The 50/10 protocol produced earlier and more sustained vascular alterations, including reduced vascular coverage, increased vascular tortuosity, and neovascularization beginning at P14. Similar vascular changes appeared later and were less persistent in the 80/40 model. Retinal thinning occurred in both models; however, long-term photoreceptor segment thinning was observed only in the 50/10 protocol. The 50/10 protocol was associated with increased retinal cell death, significant cone photoreceptor loss, and mostly reduced electroretinographic responses, findings that were not observed in the 80/40 protocol.Conclusions
Both OIR models replicate features of human pathology, but differ in disease dynamics. These differences should be considered when selecting an OIR protocol, depending on whether vascular pathology or neurodegeneration is the primary therapeutic target.
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BibTeX
@article{picard2026comparison,
title = {{Comparison of Albino and Pigmented Rat Oxygen-Induced Retinopathy Models: From Vascular to Neural Retinal Changes.}},
author = {Emilie Picard and Giulia Marchione and Emilie Batté and Jingyi Zhang and Seiki Achiedo and Marie Clavel and Amélie Brocart and Dominique Bremond-Gignac and Francine Behar-Cohen and Elsa Kermorvant and Marianne Berdugo and Alejandra Daruich},
journal = {Investigative ophthalmology \& visual science},
year = {2026},
month = oct,
doi = {10.1167/iovs.67.12.79},
url = {https://europepmc.org/article/MED/42961306},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Comparison of Albino and Pigmented Rat Oxygen-Induced Retinopathy Models: From Vascular to Neural Retinal Changes.
AU - Emilie Picard
AU - Giulia Marchione
AU - Emilie Batté
AU - Jingyi Zhang
AU - Seiki Achiedo
AU - Marie Clavel
AU - Amélie Brocart
AU - Dominique Bremond-Gignac
AU - Francine Behar-Cohen
AU - Elsa Kermorvant
AU - Marianne Berdugo
AU - Alejandra Daruich
PY - 2026
DA - 2026/10/01
JO - Investigative ophthalmology & visual science
DO - 10.1167/iovs.67.12.79
UR - https://europepmc.org/article/MED/42961306
AB - Purpose To compare two commonly used rat models of oxygen-induced retinopathy (OIR)-the 50/10 model in Sprague-Dawley rats and the 80/40 model in Long-Evans rats-to identify which more closely reproduces the features of human retinopathy.Methods Dams and pups were exposed from postnatal day 0 (P0) to P14 to alternating oxygen cycles, followed by analyses at P14, P18, and P28. The 50/10 protocol alternated 50% and 10% oxygen every 24 hours, whereas the 80/40 protocol alternated 80% and 40% oxygen every 12 hours. The vascular, structural, and functional retinal changes were assessed using in vivo imaging and electrophysiology, as well as ex vivo histological and molecular analyses.Results Both OIR protocols induced significant retinal vascular abnormalities compared with controls, but with marked differences in onset, severity, and persistence. The 50/10 protocol produced earlier and more sustained vascular alterations, including reduced vascular coverage, increased vascular tortuosity, and neovascularization beginning at P14. Similar vascular changes appeared later and were less persistent in the 80/40 model. Retinal thinning occurred in both models; however, long-term photoreceptor segment thinning was observed only in the 50/10 protocol. The 50/10 protocol was associated with increased retinal cell death, significant cone photoreceptor loss, and mostly reduced electroretinographic responses, findings that were not observed in the 80/40 protocol.Conclusions Both OIR models replicate features of human pathology, but differ in disease dynamics. These differences should be considered when selecting an OIR protocol, depending on whether vascular pathology or neurodegeneration is the primary therapeutic target.
SN - 0146-0404
SN - 1552-5783
ER -