Multimodal Evaluation of the Anti-Adhesion Effects of Heparin-Loaded PLGA/HA Nanofiber Membranes in an Intraperitoneal Adhesion Model in Rats.
9 affiliations
- 1Department of Anatomy, Faculty of Medicine, Inonu University, Malatya 44050, Türkiye.
- 2Department of Medical Biochemistry, Faculty of Medicine, Inonu University, Malatya 44050, Türkiye.
- 3Department of Chemistry, Faculty of Arts and Sciences, Inonu University, Malatya 44050, Türkiye.
- 4Department of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ 23119, Türkiye.
- 5Department of Radiology, Faculty of Medicine, Inonu University, Malatya 44050, Türkiye.
- 6Department of Anatomy, Faculty of Medicine, Malatya Turgut Özal University, Malatya 44210, Türkiye.
- 7Department of Family Medicine, Faculty of Medicine, Inonu University, Malatya 44050, Türkiye.
- 8Department of Medical History and Ethics, Faculty of Medicine, Inonu University, Malatya 44050, Türkiye.
- 9Faculty of Dentistry, Bingöl University, Bingöl 12000, Türkiye.
Abstract
This study aimed to produce and characterize nanofiber membranes (PLGA/HA@LMWH) containing Poly(lactic-co-glycolic acid)/Hyaluronic Acid (PLGA/HA) immobilized with low-molecular-weight heparin (LMWH), and to investigate their physical and chemical effects in preventing intraabdominal adhesions in a rat model using multidisciplinary methods. Forty female Wistar Albino rats were divided into four groups (n = 10). Cecal abrasions were induced in three groups: treated with PLGA/HA membrane, PLGA/HA@LMWH membrane, and 0.9% NaCl (SS). The fourth group underwent laparotomy only. On day 21, rats were sacrificed and evaluated macroscopically, histopathologically, immunohistochemically, and biochemically. Macroscopic adhesion, inflammation, fibrosis, neovascularization, collagen scores, and TGF-β1 expression were found to be high in the SS and PLGA/HA groups, while a significant decrease (p < 0.05-0.01) was detected in all of these values in the PLGA/HA@LMWH group. NF-κB activation was significantly decreased in the PLGA/HA and especially the PLGA/HA@LMWH groups compared to the SS group (p < 0.05). In addition, apoptosis was suppressed in the PLGA/HA@LMWH group, with increased Bcl-2 and decreased cleaved caspase-3. The PLGA/HA@LMWH membrane reduced peritoneal adhesions by suppressing inflammation, fibrosis, neovascularization, and collagen deposition, lowering TGF-β1 expression and NF-κB and p-NF-κB levels, and suppressing apoptosis. However, large-scale studies are needed for clinical use.
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BibTeX
@article{karatas2026multimodal,
title = {{Multimodal Evaluation of the Anti-Adhesion Effects of Heparin-Loaded PLGA/HA Nanofiber Membranes in an Intraperitoneal Adhesion Model in Rats.}},
author = {Turgay Karatas and Nurcan Gokturk and Kubra Karadas Gedik and Burak Karabulut and Damla Bilge Tasdemir and Mehmet Akif Turkoz and Ahmet Ulu and Yusuf Turkoz and Necati Timurkaan and Tugba Dogan and Anıl Kaya and Engin Burak Selcuk and Mehmet Karatas and Meryem Budak and Burhan Ates},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198715},
url = {https://europepmc.org/article/MED/42883959},
note = {CC BY},
}
RIS
TY - JOUR
TI - Multimodal Evaluation of the Anti-Adhesion Effects of Heparin-Loaded PLGA/HA Nanofiber Membranes in an Intraperitoneal Adhesion Model in Rats.
AU - Turgay Karatas
AU - Nurcan Gokturk
AU - Kubra Karadas Gedik
AU - Burak Karabulut
AU - Damla Bilge Tasdemir
AU - Mehmet Akif Turkoz
AU - Ahmet Ulu
AU - Yusuf Turkoz
AU - Necati Timurkaan
AU - Tugba Dogan
AU - Anıl Kaya
AU - Engin Burak Selcuk
AU - Mehmet Karatas
AU - Meryem Budak
AU - Burhan Ates
PY - 2026
DA - 2026/09/29
JO - International journal of molecular sciences
DO - 10.3390/ijms27198715
UR - https://europepmc.org/article/MED/42883959
AB - This study aimed to produce and characterize nanofiber membranes (PLGA/HA@LMWH) containing Poly(lactic-co-glycolic acid)/Hyaluronic Acid (PLGA/HA) immobilized with low-molecular-weight heparin (LMWH), and to investigate their physical and chemical effects in preventing intraabdominal adhesions in a rat model using multidisciplinary methods. Forty female Wistar Albino rats were divided into four groups (n = 10). Cecal abrasions were induced in three groups: treated with PLGA/HA membrane, PLGA/HA@LMWH membrane, and 0.9% NaCl (SS). The fourth group underwent laparotomy only. On day 21, rats were sacrificed and evaluated macroscopically, histopathologically, immunohistochemically, and biochemically. Macroscopic adhesion, inflammation, fibrosis, neovascularization, collagen scores, and TGF-β1 expression were found to be high in the SS and PLGA/HA groups, while a significant decrease (p < 0.05-0.01) was detected in all of these values in the PLGA/HA@LMWH group. NF-κB activation was significantly decreased in the PLGA/HA and especially the PLGA/HA@LMWH groups compared to the SS group (p < 0.05). In addition, apoptosis was suppressed in the PLGA/HA@LMWH group, with increased Bcl-2 and decreased cleaved caspase-3. The PLGA/HA@LMWH membrane reduced peritoneal adhesions by suppressing inflammation, fibrosis, neovascularization, and collagen deposition, lowering TGF-β1 expression and NF-κB and p-NF-κB levels, and suppressing apoptosis. However, large-scale studies are needed for clinical use.
SN - 1422-0067
ER -