Evaluation of housefly (Musca domestica) larvae meal and frass as alternative protein sources in late-phase laying hens: Effects on ileal digestibility and energy utilization.
4 affiliations
- 1Animal Science Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, 6715685423, Iran.
- 2Animal Science Department, College of Agriculture and Natural Resources, Razi University, Kermanshah, 6715685423, Iran. Electronic address: [email protected].
- 3Department of Plant Protection, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
- 4Department of Animal and Poultry Science, Faculty of Agricultural Technology, University of Tehran, Pakdasht, Iran.
Abstract
The present study evaluated the effects of housefly (Musca domestica) larvae meal (HFLM) and housefly larvae plus frass (HFLF), with or without a commercial synbiotic-enzyme supplement, on apparent ileal nutrient digestibility and apparent ileal digestible energy (AIDE) in Lohmann LSL-Lite laying hens. Two 10-week feeding trials were conducted, each involving 192 hens at 56 weeks of age, using a 4 × 2 factorial design with four inclusion levels (0, 5, 10, and 15%) of HFLM or HFLF and two synbiotic-enzyme supplementation levels (0 and 0.05%). In Experiment 1, HFLM inclusion did not affect nutrient digestibility or AIDE (P > 0.05), and no HFLM × synbiotic-enzyme interactions were observed. Synbiotic-enzyme supplementation reduced ash digestibility by 24.1% (42.20 vs. 55.63%; P = 0.0312), whereas it increased nitrogen-free extract digestibility (NFE) by 37.1% (72.81 vs. 53.09%; P = 0.0099) and AIDE by 23.2% (2836.2 vs. 2301.5 kcal/kg; P = 0.0240). In Experiment 2, significant HFLF × synbiotic-enzyme interactions were detected for crude protein (CP), ether extract (EE), ash, NFE, and AIDE (P ≤ 0.0401). The 10% HFLF diet supplemented with 0.05% synbiotic-enzyme produced the highest CP (87.49%), EE (79.15%), ash (70.72%), and NFE (87.49%) digestibility values and AIDE (3402.29 kcal/kg), representing increases of 42.3%, 99.8%, 69.6%, 116.8%, and 96.1%, respectively, relative to the corresponding lowest observed values. The lowest CP, NFE, and AIDE values were observed with 5% HFLF without supplementation, whereas the lowest EE and ash digestibility occurred with 15% HFLF with and without supplementation, respectively. HFLF inclusion also affected crude fiber (P = 0.0100) and calcium (P = 0.0166) digestibility, which increased by 36.4% and 52.4%, respectively, at 10% HFLF compared with the control. Overall, under the conditions of the present study, HFLM inclusion up to 15% did not adversely affect apparent ileal nutrient digestibility or energy utilization. The responses to HFLF varied with synbiotic-enzyme supplementation, with 10% HFLF plus 0.05% supplementation showing favorable nutrient digestibility and energy utilization responses.
Related
- Partial Replacement of Spray-Dried Blood Plasma with Alternative Animal and Insect Protein in Weaned Pig Diets: Effects on Growth Performance, Digestibility, and Gut Health.
- A Comprehensive Assessment of Replacing Soybean Meal with Candida Utilis-Fermented Corn Steep Liquor in Broiler Diets: Nutritional Evaluation, Growth Performance, Nutrient Digestibility, Intestinal Health and Serum Biochemical.
- Evaluation of mineral digestibility and physiological status in adult dogs fed an organic mineral-based diet.
- Evaluation of in vitro method for estimating in vivo digestibility in mixed-breed dogs across each life stage.
- Comparative evaluation of methods for measuring standardized ileal digestibility of amino acids for different sorghum varieties in broilers.
BibTeX
@article{salehizadeh2026evaluation,
title = {{Evaluation of housefly (Musca domestica) larvae meal and frass as alternative protein sources in late-phase laying hens: Effects on ileal digestibility and energy utilization.}},
author = {Abolfazl Salehizadeh and Mehran Torki and Maryam Darbemamieh and Seyed Davood Sharifi},
journal = {Poultry science},
year = {2026},
month = oct,
doi = {10.1016/j.psj.2026.107904},
url = {https://europepmc.org/article/MED/42868856},
note = {CC BY},
}
RIS
TY - JOUR
TI - Evaluation of housefly (Musca domestica) larvae meal and frass as alternative protein sources in late-phase laying hens: Effects on ileal digestibility and energy utilization.
AU - Abolfazl Salehizadeh
AU - Mehran Torki
AU - Maryam Darbemamieh
AU - Seyed Davood Sharifi
PY - 2026
DA - 2026/10/05
JO - Poultry science
DO - 10.1016/j.psj.2026.107904
UR - https://europepmc.org/article/MED/42868856
AB - The present study evaluated the effects of housefly (Musca domestica) larvae meal (HFLM) and housefly larvae plus frass (HFLF), with or without a commercial synbiotic-enzyme supplement, on apparent ileal nutrient digestibility and apparent ileal digestible energy (AIDE) in Lohmann LSL-Lite laying hens. Two 10-week feeding trials were conducted, each involving 192 hens at 56 weeks of age, using a 4 × 2 factorial design with four inclusion levels (0, 5, 10, and 15%) of HFLM or HFLF and two synbiotic-enzyme supplementation levels (0 and 0.05%). In Experiment 1, HFLM inclusion did not affect nutrient digestibility or AIDE (P > 0.05), and no HFLM × synbiotic-enzyme interactions were observed. Synbiotic-enzyme supplementation reduced ash digestibility by 24.1% (42.20 vs. 55.63%; P = 0.0312), whereas it increased nitrogen-free extract digestibility (NFE) by 37.1% (72.81 vs. 53.09%; P = 0.0099) and AIDE by 23.2% (2836.2 vs. 2301.5 kcal/kg; P = 0.0240). In Experiment 2, significant HFLF × synbiotic-enzyme interactions were detected for crude protein (CP), ether extract (EE), ash, NFE, and AIDE (P ≤ 0.0401). The 10% HFLF diet supplemented with 0.05% synbiotic-enzyme produced the highest CP (87.49%), EE (79.15%), ash (70.72%), and NFE (87.49%) digestibility values and AIDE (3402.29 kcal/kg), representing increases of 42.3%, 99.8%, 69.6%, 116.8%, and 96.1%, respectively, relative to the corresponding lowest observed values. The lowest CP, NFE, and AIDE values were observed with 5% HFLF without supplementation, whereas the lowest EE and ash digestibility occurred with 15% HFLF with and without supplementation, respectively. HFLF inclusion also affected crude fiber (P = 0.0100) and calcium (P = 0.0166) digestibility, which increased by 36.4% and 52.4%, respectively, at 10% HFLF compared with the control. Overall, under the conditions of the present study, HFLM inclusion up to 15% did not adversely affect apparent ileal nutrient digestibility or energy utilization. The responses to HFLF varied with synbiotic-enzyme supplementation, with 10% HFLF plus 0.05% supplementation showing favorable nutrient digestibility and energy utilization responses.
SN - 0032-5791
SN - 1525-3171
ER -