Effects of low-level Er:YAG laser irradiation on endothelial angiogenic responses in diabetes: An in vitro study.
5 affiliations
- 1Department of Periodontology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.
- 2Department of General Dentistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.
- 3Department of Oral Biomedical Sciences, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.
- 4School of Dentistry, Chung Shan Medical University (CSMU), Taichung, Taiwan.
- 5Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
Abstract
Background/purpose
Low-level Er:YAG laser irradiation has been reported to promote angiogenesis-related endothelial responses and enhance periodontal tissue healing. However, its effects on angiogenesis under hyperglycemic conditions have not been investigated. This study aimed to investigate whether low-level Er:YAG laser irradiation enhances the angiogenic response under hyperglycemic conditions.Materials and methods
Human umbilical vein endothelial cells (HUVECs) were cultured in control-glucose (CG, 5.5 mM) or high-glucose (HG, 25 mM) medium and subjected to low-level laser irradiation (LLLI) using an Er:YAG laser at 10 Hz and 30 mJ/pulse for 30 s under two pulse-widths. Angiogenesis-related cellular functions were evaluated by assessing cell proliferation, migration, tube formation, and mRNA expression. Intracellular reactive oxygen species (ROS) levels were measured.Results
Although cell proliferation and migration significantly decreased under HG conditions (P < 0.01, P < 0.001), both were significantly restored after LLLI, reaching levels comparable to or higher than those in the HG control group. Under HG conditions, tube formation decreased; however, after LLLI, tube formation recovered, with significantly increased numbers of nodes, junctions, and segments, compared to those in the CG control group (P < 0.05). mRNA expression was not affected by HG medium, but vascular endothelial growth factor (VEGF ) and angiopoietin 1 (ANGPT1) expression significantly increased following LLLI (P < 0.05). ROS levels were significantly increased in the HG control group (P < 0.001), whereas the laser-treated groups showed significantly reduced ROS levels under HG conditions (P < 0.01).Conclusion
Low-level Er:YAG laser irradiation improved angiogenesis-related cellular functions under hyperglycemic conditions.
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BibTeX
@article{moreira2026effects,
title = {{Effects of low-level Er:YAG laser irradiation on endothelial angiogenic responses in diabetes: An in vitro study.}},
author = {Bruna Akinaga Moreira and Koji Mizutani and Natsumi Saito and Lu Chen and Shu Takemura and Keita Nakagawa and Yujin Ohsugi and Taichen Lin and Takanori Iwata and Akira Aoki},
journal = {Journal of dental sciences},
year = {2026},
month = oct,
doi = {10.1016/j.jds.2025.07.3585},
url = {https://europepmc.org/article/MED/42923741},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Effects of low-level Er:YAG laser irradiation on endothelial angiogenic responses in diabetes: An in vitro study.
AU - Bruna Akinaga Moreira
AU - Koji Mizutani
AU - Natsumi Saito
AU - Lu Chen
AU - Shu Takemura
AU - Keita Nakagawa
AU - Yujin Ohsugi
AU - Taichen Lin
AU - Takanori Iwata
AU - Akira Aoki
PY - 2026
DA - 2026/10/01
JO - Journal of dental sciences
DO - 10.1016/j.jds.2025.07.3585
UR - https://europepmc.org/article/MED/42923741
AB - Background/purpose Low-level Er:YAG laser irradiation has been reported to promote angiogenesis-related endothelial responses and enhance periodontal tissue healing. However, its effects on angiogenesis under hyperglycemic conditions have not been investigated. This study aimed to investigate whether low-level Er:YAG laser irradiation enhances the angiogenic response under hyperglycemic conditions.Materials and methods Human umbilical vein endothelial cells (HUVECs) were cultured in control-glucose (CG, 5.5 mM) or high-glucose (HG, 25 mM) medium and subjected to low-level laser irradiation (LLLI) using an Er:YAG laser at 10 Hz and 30 mJ/pulse for 30 s under two pulse-widths. Angiogenesis-related cellular functions were evaluated by assessing cell proliferation, migration, tube formation, and mRNA expression. Intracellular reactive oxygen species (ROS) levels were measured.Results Although cell proliferation and migration significantly decreased under HG conditions (P < 0.01, P < 0.001), both were significantly restored after LLLI, reaching levels comparable to or higher than those in the HG control group. Under HG conditions, tube formation decreased; however, after LLLI, tube formation recovered, with significantly increased numbers of nodes, junctions, and segments, compared to those in the CG control group (P < 0.05). mRNA expression was not affected by HG medium, but vascular endothelial growth factor (VEGF ) and angiopoietin 1 (ANGPT1) expression significantly increased following LLLI (P < 0.05). ROS levels were significantly increased in the HG control group (P < 0.001), whereas the laser-treated groups showed significantly reduced ROS levels under HG conditions (P < 0.01).Conclusion Low-level Er:YAG laser irradiation improved angiogenesis-related cellular functions under hyperglycemic conditions.
SN - 1991-7902
SN - 2213-8862
ER -