PreprintCC BY 4.0arXiv
From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process
Abstract
Light sheet microscopy is ideal for the 3D imaging of large biological specimens, yet systematic documentation on adapting microscope designs to diverse experimental requirements remains limited. We present the iterative process used to design and build a modular light-sheet microscope and adapt it to different sample types and experimental conditions. Starting from a stripped-down OpenSPIM platform, we gradually make our microscope more versatile by implementing new hardware, such as additional laser channels and optical filters, and new protocols for sample handling. In its final iteration, FlowSPIM is a new flow-based incubation technique for 3D tissue culture.
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BibTeX
@misc{mossige2026from,
title = {{From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process}},
author = {Endre Joachim Lerheim Mossige and Sergey Ponomartsev and Natalia Smirnova and Wietske van der Ent and Siri Andresen and Xian Hu and Felix Margadant and Rainer Heintzmann and Roland Kádár and Kesavan Sekar and Helene Knævelsrud and Camila Vicencio Esguerra and Stefan Krauss and Dag Kristian Dysthe and Alexander Refsum Jensenius},
howpublished = {arXiv},
year = {2026},
month = sep,
doi = {10.48550/arxiv.2609.31115},
eprint = {2609.31115},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2609.31115},
note = {CC BY 4.0},
}
RIS
TY - UNPB
TI - From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process
AU - Endre Joachim Lerheim Mossige
AU - Sergey Ponomartsev
AU - Natalia Smirnova
AU - Wietske van der Ent
AU - Siri Andresen
AU - Xian Hu
AU - Felix Margadant
AU - Rainer Heintzmann
AU - Roland Kádár
AU - Kesavan Sekar
AU - Helene Knævelsrud
AU - Camila Vicencio Esguerra
AU - Stefan Krauss
AU - Dag Kristian Dysthe
AU - Alexander Refsum Jensenius
PY - 2026
DA - 2026/09/25
PB - arXiv
DO - 10.48550/arxiv.2609.31115
UR - https://arxiv.org/abs/2609.31115
AB - Light sheet microscopy is ideal for the 3D imaging of large biological specimens, yet systematic documentation on adapting microscope designs to diverse experimental requirements remains limited. We present the iterative process used to design and build a modular light-sheet microscope and adapt it to different sample types and experimental conditions. Starting from a stripped-down OpenSPIM platform, we gradually make our microscope more versatile by implementing new hardware, such as additional laser channels and optical filters, and new protocols for sample handling. In its final iteration, FlowSPIM is a new flow-based incubation technique for 3D tissue culture.
ER -