A miniature bioluminescent platform for imaging structured RNAs
- 1University of California Irvine
Abstract
A broader understanding of RNA biology requires methods for visualizing transcript dynamics in native environments. Common imaging approaches rely on fluorescent probes that require excitation light and often depend on large aptamer arrays, limiting sensitivity and compatibility with many non-coding RNAs, particularly highly structured folds. To address these challenges, we developed a miniaturized bioluminescent platform for RNA imaging. This system employs a compact RNA tag that assembles luciferase fragments upon binding of monomeric RNA-binding proteins (RNA lanterns). Systematic optimization of the RNA component yielded a structured 24-nucleotide tag, representing greater than 3-fold reduction in size relative to the original C-11-B RNA tag, placing it among the smallest RNA imaging tags reported to date. The optimized tag enabled RNA-dependent bioluminescence detection in live cells, notably as a small, single insert within highly structured non-coding RNAs, including the MALAT1 ENE domain and a small HDV-like ribozyme. Collectively, this work establishes a miniaturized bioluminescent platform for imaging a broad range of RNA targets in living systems.
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BibTeX
@misc{chan2026miniature,
title = {{A miniature bioluminescent platform for imaging structured RNAs}},
author = {C. E. Chan and E. B. Fuller and C. C. Chen and O. Rabie and N. T. Seto and O. Steward and J. A. Prescher and A. Luptak},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.01.755752},
url = {https://www.biorxiv.org/content/10.64898/2026.10.01.755752v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - A miniature bioluminescent platform for imaging structured RNAs
AU - C. E. Chan
AU - E. B. Fuller
AU - C. C. Chen
AU - O. Rabie
AU - N. T. Seto
AU - O. Steward
AU - J. A. Prescher
AU - A. Luptak
PY - 2026
DA - 2026/10/01
PB - bioRxiv
DO - 10.64898/2026.10.01.755752
UR - https://www.biorxiv.org/content/10.64898/2026.10.01.755752v1
AB - A broader understanding of RNA biology requires methods for visualizing transcript dynamics in native environments. Common imaging approaches rely on fluorescent probes that require excitation light and often depend on large aptamer arrays, limiting sensitivity and compatibility with many non-coding RNAs, particularly highly structured folds. To address these challenges, we developed a miniaturized bioluminescent platform for RNA imaging. This system employs a compact RNA tag that assembles luciferase fragments upon binding of monomeric RNA-binding proteins (RNA lanterns). Systematic optimization of the RNA component yielded a structured 24-nucleotide tag, representing greater than 3-fold reduction in size relative to the original C-11-B RNA tag, placing it among the smallest RNA imaging tags reported to date. The optimized tag enabled RNA-dependent bioluminescence detection in live cells, notably as a small, single insert within highly structured non-coding RNAs, including the MALAT1 ENE domain and a small HDV-like ribozyme. Collectively, this work establishes a miniaturized bioluminescent platform for imaging a broad range of RNA targets in living systems.
ER -