American Ginseng Protein-Enriched Fraction Attenuates Neurobehavioral Deficits in MPTP-Induced Parkinson’s Disease Mice with Associated Modulation of Gut Microbiota and Metabolic Pathways
- 1Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
- 2College of Pharmacy of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
Abstract
Background/Objectives: Parkinson’s disease (PD) currently lacks disease-modifying therapies. Available treatments offer only symptomatic relief and fail to halt progressive dopaminergic neuron loss. American ginseng (Panax quinquefolius L.) is a well-recognized functional food with documented neuroprotective properties, largely ascribed to its ginsenoside and polysaccharide fractions. However, its endogenous protein component, the American ginseng protein-enriched fraction (AGP), remains underexplored in the context of PD. This study aimed to investigate the neuroprotective effects of dietary AGP on PD and its association with the microbiota–gut–brain axis (MGBA). Methods: AGP was purified, structurally characterized, and then orally administered to male C57BL/6 mice with MPTP-induced PD for 28 days. Multi-omics analyses and Western blotting were employed to evaluate its efficacy and explore potential underlying mechanisms. Results: AGP treatment significantly improved motor function and place navigation performance in the Morris water maze, restored striatal dopamine levels, and reduced α-synuclein (α-Syn) aggregation in PD mice. In addition, AGP was associated with suppression of the TLR4/MyD88/NF-κB/ERK inflammatory pathway, the reshaping of the dysregulated gut microbiota composition, and the correction of metabolic disorders in the sphingolipid and arachidonic acid pathways. Conclusions: AGP was associated with significantly improved neuroprotective effects in PD mice, accompanied by concurrent modulations of the gut microbiota, metabolic profiles, and inflammatory signaling. These associations suggest potential MGBA involvement, although directionality requires further investigation. These findings support further investigation of AGP as a candidate dietary intervention for neurodegenerative diseases.
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BibTeX
@article{li2026american,
title = {{American Ginseng Protein-Enriched Fraction Attenuates Neurobehavioral Deficits in MPTP-Induced Parkinson’s Disease Mice with Associated Modulation of Gut Microbiota and Metabolic Pathways}},
author = {Jie Li and Xu Yang and Jiahan Wang and Chunhui Yu and Jiahong Lu and Wenhui Lian and Wei Liu and Yu Zhao},
journal = {Nutrients},
year = {2026},
month = oct,
doi = {10.3390/nu18203412},
url = {https://www.mdpi.com/2072-6643/18/20/3412},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - American Ginseng Protein-Enriched Fraction Attenuates Neurobehavioral Deficits in MPTP-Induced Parkinson’s Disease Mice with Associated Modulation of Gut Microbiota and Metabolic Pathways
AU - Jie Li
AU - Xu Yang
AU - Jiahan Wang
AU - Chunhui Yu
AU - Jiahong Lu
AU - Wenhui Lian
AU - Wei Liu
AU - Yu Zhao
PY - 2026
DA - 2026/10/01
JO - Nutrients
DO - 10.3390/nu18203412
UR - https://www.mdpi.com/2072-6643/18/20/3412
AB - Background/Objectives: Parkinson’s disease (PD) currently lacks disease-modifying therapies. Available treatments offer only symptomatic relief and fail to halt progressive dopaminergic neuron loss. American ginseng (Panax quinquefolius L.) is a well-recognized functional food with documented neuroprotective properties, largely ascribed to its ginsenoside and polysaccharide fractions. However, its endogenous protein component, the American ginseng protein-enriched fraction (AGP), remains underexplored in the context of PD. This study aimed to investigate the neuroprotective effects of dietary AGP on PD and its association with the microbiota–gut–brain axis (MGBA). Methods: AGP was purified, structurally characterized, and then orally administered to male C57BL/6 mice with MPTP-induced PD for 28 days. Multi-omics analyses and Western blotting were employed to evaluate its efficacy and explore potential underlying mechanisms. Results: AGP treatment significantly improved motor function and place navigation performance in the Morris water maze, restored striatal dopamine levels, and reduced α-synuclein (α-Syn) aggregation in PD mice. In addition, AGP was associated with suppression of the TLR4/MyD88/NF-κB/ERK inflammatory pathway, the reshaping of the dysregulated gut microbiota composition, and the correction of metabolic disorders in the sphingolipid and arachidonic acid pathways. Conclusions: AGP was associated with significantly improved neuroprotective effects in PD mice, accompanied by concurrent modulations of the gut microbiota, metabolic profiles, and inflammatory signaling. These associations suggest potential MGBA involvement, although directionality requires further investigation. These findings support further investigation of AGP as a candidate dietary intervention for neurodegenerative diseases.
SN - 2072-6643
ER -