A glyoxal sensing Pseudomonas aeruginosa transcription factor enables lung infection
- 1Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Abstract
Aldehydes are a class of normally unwanted toxic electrophilic compounds that mainly arise from oxidation of glucose, lipids or DNA. However, it has recently come to light that they can also be weaponized by professional phagocytes to kill engulfed bacteria. How microbes subvert these assaults remains largely enigmatic. Here we describe the function, atomic structure and mechanism of the first bacterial transcription factor able to directly sense the dicarbonyl glyoxal (GO), which we aptly named the Glyoxal Regulator (GloR, from Pseudomonas aeruginosa PAO1), We show that GloR directly senses GO through a reversible cysteine modification that results in its binding to a conserved DNA regulatory motif (a glo box), which then triggers a transcriptional activation of a defined set of genes to help counter GO toxicity and enable acute lung infection. Despite substantial evolutionary divergence, when unmodified gloR and a glo box-regulated reporter were transferred into E. coli, a strikingly tight GO-specific regulation was maintained, suggesting this system could be readily transferred between unrelated microbial species. As homologs of GloR were identified in diverse bacterial species we anticipate its use to be widespread in both pathogens and environmental bacteria. Taken together, we present the first bona fide bacterial aldehyde regulator which senses host GO to enable survival during infection.
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BibTeX
@misc{corcoran2026glyoxal,
title = {{A glyoxal sensing Pseudomonas aeruginosa transcription factor enables lung infection}},
author = {C. J. Corcoran and D. G. Glanville and B. J. Cuthbert and R. de Miranda and J. Martinez and J. D. Keith and P. Prevelige and S. E. Birket and C. W. Goulding and A. T. Ulijasz},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.20.752901},
url = {https://www.biorxiv.org/content/10.64898/2026.09.20.752901v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - A glyoxal sensing Pseudomonas aeruginosa transcription factor enables lung infection
AU - C. J. Corcoran
AU - D. G. Glanville
AU - B. J. Cuthbert
AU - R. de Miranda
AU - J. Martinez
AU - J. D. Keith
AU - P. Prevelige
AU - S. E. Birket
AU - C. W. Goulding
AU - A. T. Ulijasz
PY - 2026
DA - 2026/09/20
PB - bioRxiv
DO - 10.64898/2026.09.20.752901
UR - https://www.biorxiv.org/content/10.64898/2026.09.20.752901v1
AB - Aldehydes are a class of normally unwanted toxic electrophilic compounds that mainly arise from oxidation of glucose, lipids or DNA. However, it has recently come to light that they can also be weaponized by professional phagocytes to kill engulfed bacteria. How microbes subvert these assaults remains largely enigmatic. Here we describe the function, atomic structure and mechanism of the first bacterial transcription factor able to directly sense the dicarbonyl glyoxal (GO), which we aptly named the Glyoxal Regulator (GloR, from Pseudomonas aeruginosa PAO1), We show that GloR directly senses GO through a reversible cysteine modification that results in its binding to a conserved DNA regulatory motif (a glo box), which then triggers a transcriptional activation of a defined set of genes to help counter GO toxicity and enable acute lung infection. Despite substantial evolutionary divergence, when unmodified gloR and a glo box-regulated reporter were transferred into E. coli, a strikingly tight GO-specific regulation was maintained, suggesting this system could be readily transferred between unrelated microbial species. As homologs of GloR were identified in diverse bacterial species we anticipate its use to be widespread in both pathogens and environmental bacteria. Taken together, we present the first bona fide bacterial aldehyde regulator which senses host GO to enable survival during infection.
ER -