Antibacterial Activity of Equisetin from Endophytic Fusarium incarnatum JMB4 Associated with Jatropha multifida L. Against MRSA: Experimental and In Silico Insights
- 1National Cheng Kung University , , 1 University Road , ,
- 2Universitas Andalas , , Kampus Limau Manis , ,
- 3Akademi Farmasi Cendikia Farma Husada , Jl. Pulau Enggano–Tirtayasa No. 100, Sukabumi , ,
Abstract
An antibacterial metabolite was isolated from the endophytic fungus Fusarium incarnatum JMB4 associated with Jatropha multifida L. by bioactivity-guided chromatography and recrystallization. The isolated compound was assigned as equisetin using UV–vis, FTIR, LC–MS/MS, and 1H NMR data. Anti-MRSA activity was evaluated by disk diffusion, with chloramphenicol and amoxicillin as antibiotic comparators. At 1% (w/v; 100 μg/disk), equisetin produced an inhibition zone of 23.94 ± 0.66 mm; the recorded comparator zones were 24.71 ± 0.68 mm for chloramphenicol (30 μg/disk) and 11.76 ± 0.66 mm for amoxicillin (25 μg/disk). The lowest tested equisetin concentration producing a measurable zone was 78.1 μg/mL (7.03 ± 0.25 mm). This diffusion end point does not represent a minimum inhibitory concentration (MIC), and the unequal disk loads preclude a direct comparison of intrinsic antibacterial potency. Exploratory docking against penicillin-binding protein 2a (PBP2a) yielded a score of −5.5041 kcal/mol and a predicted pose outside the canonical catalytic pocket. A 100 ns molecular dynamics simulation described the behavior of the modeled complex but did not establish PBP2a inhibition. These results document anti-MRSA activity of the isolated compound under the tested conditions; standardized MIC determination and further structural validation remain necessary.
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BibTeX
@article{satriawan2026antibacterial,
title = {{Antibacterial Activity of Equisetin from Endophytic Fusarium incarnatum JMB4 Associated with Jatropha multifida L. Against MRSA: Experimental and In Silico Insights}},
author = {Herland Satriawan and Rachel Shammara Khalda and Hao-Ze Li and Arif Setiawansyah and Ping-Chung Kuo and Dian Handayani},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c07232},
url = {https://pubs.acs.org/acsodf/article/11/41/63520/5445676/Antibacterial-Activity-of-Equisetin-from},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Antibacterial Activity of Equisetin from Endophytic Fusarium incarnatum JMB4 Associated with Jatropha multifida L. Against MRSA: Experimental and In Silico Insights
AU - Herland Satriawan
AU - Rachel Shammara Khalda
AU - Hao-Ze Li
AU - Arif Setiawansyah
AU - Ping-Chung Kuo
AU - Dian Handayani
PY - 2026
DA - 2026/10/06
JO - ACS Omega
DO - 10.1021/acsomega.6c07232
UR - https://pubs.acs.org/acsodf/article/11/41/63520/5445676/Antibacterial-Activity-of-Equisetin-from
AB - An antibacterial metabolite was isolated from the endophytic fungus Fusarium incarnatum JMB4 associated with Jatropha multifida L. by bioactivity-guided chromatography and recrystallization. The isolated compound was assigned as equisetin using UV–vis, FTIR, LC–MS/MS, and 1H NMR data. Anti-MRSA activity was evaluated by disk diffusion, with chloramphenicol and amoxicillin as antibiotic comparators. At 1% (w/v; 100 μg/disk), equisetin produced an inhibition zone of 23.94 ± 0.66 mm; the recorded comparator zones were 24.71 ± 0.68 mm for chloramphenicol (30 μg/disk) and 11.76 ± 0.66 mm for amoxicillin (25 μg/disk). The lowest tested equisetin concentration producing a measurable zone was 78.1 μg/mL (7.03 ± 0.25 mm). This diffusion end point does not represent a minimum inhibitory concentration (MIC), and the unequal disk loads preclude a direct comparison of intrinsic antibacterial potency. Exploratory docking against penicillin-binding protein 2a (PBP2a) yielded a score of −5.5041 kcal/mol and a predicted pose outside the canonical catalytic pocket. A 100 ns molecular dynamics simulation described the behavior of the modeled complex but did not establish PBP2a inhibition. These results document anti-MRSA activity of the isolated compound under the tested conditions; standardized MIC determination and further structural validation remain necessary.
SN - 2470-1343
ER -