Diagnostic performance of a dual western blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies
- 1Laboratorio de Referencia Nacional de Virus de Transmisión Sexual, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Peru. Laboratorio de Referencia Nacional de Virus de Transmisión Sexual Centro Nacional de Salud Pública Instituto Nacional de Salud Lima Peru
Abstract
This research aimed to determine the performance of an in house dual western blot (WB) for the individual and simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies; the strip was designed with HTLV-1 antigen (1.67 ug/mm) on top and HIV-1 (1.34 ug/mm) on the bottom. The in-house dual WB was evaluated using 260 reference serum samples from a serum bank: 50 HTLV-1/HIV-1 positive, 50 HIV-1 positive, 60 HTLV-1 positive, 50 negative, and 50 potentially interfering samples. Accuracy was evaluated in five regions of Peru. Overall sensitivity and specificity were 96.3% (95% CI: 93.0‒99.5) and 100% (95% CI: 99.5‒100.0), respectively. Individually, sensitivity was 94% in HTLV-1/HIV-1 coinfected individuals, 96.7% in HTLV-1, and 98% in HIV-1. Repeatability and reproducibility were 100% under conditions of 34‒3256 m a.s.l., 16.4‒37.9 °C, and 42‒95% relative humidity. We conclude that the in house dual HTLV-1/HIV-1 WB shows high diagnostic performance.
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BibTeX
@article{mirandaulloa2026diagnostic,
title = {{Diagnostic performance of a dual western blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies}},
author = {Eduardo Miranda-Ulloa and Soledad Romero-Ruiz and Ronal Briceño-Espinoza and Andrés Vásquez-Domínguez and Dilan Suárez-Agüero},
journal = {Revista Peruana de Medicina Experimental y Salud Publica},
year = {2026},
month = sep,
doi = {10.17843/rpmesp.2026.433.16741},
url = {https://doi.org/10.17843/rpmesp.2026.433.16741},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Diagnostic performance of a dual western blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies
AU - Eduardo Miranda-Ulloa
AU - Soledad Romero-Ruiz
AU - Ronal Briceño-Espinoza
AU - Andrés Vásquez-Domínguez
AU - Dilan Suárez-Agüero
PY - 2026
DA - 2026/09/28
JO - Revista Peruana de Medicina Experimental y Salud Publica
DO - 10.17843/rpmesp.2026.433.16741
UR - https://doi.org/10.17843/rpmesp.2026.433.16741
AB - This research aimed to determine the performance of an in house dual western blot (WB) for the individual and simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies; the strip was designed with HTLV-1 antigen (1.67 ug/mm) on top and HIV-1 (1.34 ug/mm) on the bottom. The in-house dual WB was evaluated using 260 reference serum samples from a serum bank: 50 HTLV-1/HIV-1 positive, 50 HIV-1 positive, 60 HTLV-1 positive, 50 negative, and 50 potentially interfering samples. Accuracy was evaluated in five regions of Peru. Overall sensitivity and specificity were 96.3% (95% CI: 93.0‒99.5) and 100% (95% CI: 99.5‒100.0), respectively. Individually, sensitivity was 94% in HTLV-1/HIV-1 coinfected individuals, 96.7% in HTLV-1, and 98% in HIV-1. Repeatability and reproducibility were 100% under conditions of 34‒3256 m a.s.l., 16.4‒37.9 °C, and 42‒95% relative humidity. We conclude that the in house dual HTLV-1/HIV-1 WB shows high diagnostic performance.
SN - 1726-4634
SN - 1726-4642
ER -