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Peer-reviewedCC BY-NC-NDMethodsX

Duplex PCR method for simultaneous detection of scale drop disease virus and Vibrio harveyi in Asian seabass (Lates calcarifer)

  • Watcharachai Meemetta1
  • Suwimon Taengphu1
  • Onanong Charoenwai1
  • Sakulrat Kongmuang2
  • Molruedee Sonthi2
  • Ha Thanh Dong3
  • Saengchan Senapin1
  1. 1Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand
  2. 2Faculty of Marine Technology, Burapha University Chanthaburi Campus, Chanthaburi, 22170, Thailand
  3. 3Aquaculture and Aquatic Resources Management Program, Faculty of Food, Agriculture, and Natural Resources, Asian Institute of Technology, 12120, Thailand
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Abstract

Scale drop disease virus (SDDV) and Vibrio harveyi are important infectious agents affecting farmed Asian seabass (Lates calcarifer). Fish infected with these pathogens usually exhibit similar clinical signs, making differential diagnosis challenging. This study aimed to develop a duplex PCR protocol for the simultaneous detection of both pathogens in farmed Asian seabass. The newly developed method demonstrated high sensitivity (20 copies per reaction) and high specificity with no cross-amplification observed. It successfully detected infections in clinical samples (n = 42) and revealed co-infections in 11/42 samples. This duplex PCR protocol offers a useful molecular tool for detecting individual or concurrent infections, thereby contributing to enhanced disease diagnosis, surveillance and monitoring in Asian seabass aquaculture. • Simultaneous detection of SDDV and V. harveyi in a single PCR reaction • High sensitivity and specificity without cross-reactivity • Applicable to clinical samples for confirmatory disease diagnosis.

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Cite

BibTeXRIS
BibTeX
@article{meemetta2026duplex,
  title = {{Duplex PCR method for simultaneous detection of scale drop disease virus and Vibrio harveyi in Asian seabass (Lates calcarifer)}},
  author = {Watcharachai Meemetta and Suwimon Taengphu and Onanong Charoenwai and Sakulrat Kongmuang and Molruedee Sonthi and Ha Thanh Dong and Saengchan Senapin},
  journal = {MethodsX},
  year = {2026},
  month = oct,
  doi = {10.1016/j.mex.2026.104208},
  url = {https://europepmc.org/article/PMC/PMC13718649},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Duplex PCR method for simultaneous detection of scale drop disease virus and Vibrio harveyi in Asian seabass (Lates calcarifer)
AU  - Watcharachai Meemetta
AU  - Suwimon Taengphu
AU  - Onanong Charoenwai
AU  - Sakulrat Kongmuang
AU  - Molruedee Sonthi
AU  - Ha Thanh Dong
AU  - Saengchan Senapin
PY  - 2026
DA  - 2026/10/13
JO  - MethodsX
DO  - 10.1016/j.mex.2026.104208
UR  - https://europepmc.org/article/PMC/PMC13718649
AB  - Scale drop disease virus (SDDV) and Vibrio harveyi are important infectious agents affecting farmed Asian seabass (Lates calcarifer). Fish infected with these pathogens usually exhibit similar clinical signs, making differential diagnosis challenging. This study aimed to develop a duplex PCR protocol for the simultaneous detection of both pathogens in farmed Asian seabass. The newly developed method demonstrated high sensitivity (20 copies per reaction) and high specificity with no cross-amplification observed. It successfully detected infections in clinical samples (n = 42) and revealed co-infections in 11/42 samples. This duplex PCR protocol offers a useful molecular tool for detecting individual or concurrent infections, thereby contributing to enhanced disease diagnosis, surveillance and monitoring in Asian seabass aquaculture. • Simultaneous detection of SDDV and V. harveyi in a single PCR reaction • High sensitivity and specificity without cross-reactivity • Applicable to clinical samples for confirmatory disease diagnosis.
SN  - 2215-0161
ER  -