Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed pennycress (Thlaspi arvense)
Abstract
We investigated the impact of ethyl methanesulfonate (EMS) on genetically effective cells (GECs) to characterize the intraindividual genetic variation of EMS mutagenesis in the novel intermediate crop pennycress (Thlaspi arvense L.). We identified that pennycress contains at least four GECs which, when treated with EMS, create unique mutations across different branches within the same individual plant. However, we also found that branches were not completely distinct with 3.83% to 34.2% of the observed mutations being shared between at least two branches. We then propagated the M2 plants for whole genome sequencing, providing extensive characterization of the EMS mutation profile and developing a gene index as a resource for future reverse genetic screenings. The gene index was then validated through identification of two traits. We identified five mutations in transparent testa (TT) family of genes which resulted in lighter seed coat coloration and two mutations in the indehiscent (IND) gene which resulted in increased shatter resistance of the pennycress seedpod. These two investigations demonstrate the viability of the gene index as a resource for further reverse genetic screenings.
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BibTeX
@article{brusa2026characterization,
title = {{Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed pennycress (Thlaspi arvense)}},
author = {Anthony Brusa and Ratan Chopra and Eva Serena Gjesvold and Matthew Ott and Liam Sullivan and Claire Chapman Biel and Shweta Jain and Carl Branch and Greta Rockstad and Krishan M Rai and David Marks},
journal = {G3: Genes | Genomes | Genetics},
year = {2026},
month = aug,
doi = {10.1093/g3journal/jkag233},
url = {https://doi.org/10.1093/g3journal/jkag233},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed pennycress (Thlaspi arvense)
AU - Anthony Brusa
AU - Ratan Chopra
AU - Eva Serena Gjesvold
AU - Matthew Ott
AU - Liam Sullivan
AU - Claire Chapman Biel
AU - Shweta Jain
AU - Carl Branch
AU - Greta Rockstad
AU - Krishan M Rai
AU - David Marks
PY - 2026
DA - 2026/08/22
JO - G3: Genes | Genomes | Genetics
DO - 10.1093/g3journal/jkag233
UR - https://doi.org/10.1093/g3journal/jkag233
AB - We investigated the impact of ethyl methanesulfonate (EMS) on genetically effective cells (GECs) to characterize the intraindividual genetic variation of EMS mutagenesis in the novel intermediate crop pennycress (Thlaspi arvense L.). We identified that pennycress contains at least four GECs which, when treated with EMS, create unique mutations across different branches within the same individual plant. However, we also found that branches were not completely distinct with 3.83% to 34.2% of the observed mutations being shared between at least two branches. We then propagated the M2 plants for whole genome sequencing, providing extensive characterization of the EMS mutation profile and developing a gene index as a resource for future reverse genetic screenings. The gene index was then validated through identification of two traits. We identified five mutations in transparent testa (TT) family of genes which resulted in lighter seed coat coloration and two mutations in the indehiscent (IND) gene which resulted in increased shatter resistance of the pennycress seedpod. These two investigations demonstrate the viability of the gene index as a resource for further reverse genetic screenings.
SN - 2160-1836
ER -