Molecular and functional characterization of a typical 2-Cys peroxiredoxin and its developmental and pyriproxyfen-responsive expression in Aedes albopictus (Diptera: Culicidae)
Abstract
The Asian tiger mosquito, Aedes albopictus (Skuse, 1894) (Diptera: Culicidae), is established and expanding across the Black Sea region, but the redox processes associated with its development and response to insect growth regulators remain poorly understood. We characterized a typical 2-Cys peroxiredoxin (Prx2) by examining intraspecific sequence variation, conserved protein features, enzymatic activity, developmental transcription, and transcription following exposure to 0.06 ppb pyriproxyfen. The coding region was polymorphic among Turkish isolates, but all substitutions were synonymous, and the deduced amino acid sequence was conserved. Catalytic cysteines and hallmark motifs supported classification as a typical 2-Cys peroxiredoxin. Recombinant His-tagged Prx2 migrated at the expected molecular size and removed hydrogen peroxide in a concentration- and thiol-dependent manner. Basal transcript abundance varied across development, reaching its lowest level in pupae and higher levels in adults. Exposure to 0.06 ppb pyriproxyfen was associated with increased Prx2 transcript abundance in L3 and L4 larvae, whereas no statistically detectable treatment-related difference was observed among the surviving pupae or adults at the sampled time points. Together, these findings identify Prx2 as a conserved, functional thiol peroxidase and demonstrate a pyriproxyfen-associated larval transcriptional response under the conditions tested. Because this concentration was not independently validated as sublethal in the present colony and later-stage sampling necessarily comprised survivors of the original exposure, the data do not distinguish developmental attenuation of the response from survivor selection.
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BibTeX
@article{arslanhan2026molecular,
title = {{Molecular and functional characterization of a typical 2-Cys peroxiredoxin and its developmental and pyriproxyfen-responsive expression in Aedes albopictus (Diptera: Culicidae)}},
author = {Batuhan Askim Arslanhan and Samba Deguene Diop and Simge Sahin and Ahsen Meliha Toroslu and Alina Denis Kizgin and Gokmen Zafer Pekmezci and Abdullah Inci and Alparslan Yildirim},
journal = {Journal of Insect Science},
year = {2026},
month = oct,
doi = {10.1093/jisesa/ieag119},
url = {https://doi.org/10.1093/jisesa/ieag119},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Molecular and functional characterization of a typical 2-Cys peroxiredoxin and its developmental and pyriproxyfen-responsive expression in Aedes albopictus (Diptera: Culicidae)
AU - Batuhan Askim Arslanhan
AU - Samba Deguene Diop
AU - Simge Sahin
AU - Ahsen Meliha Toroslu
AU - Alina Denis Kizgin
AU - Gokmen Zafer Pekmezci
AU - Abdullah Inci
AU - Alparslan Yildirim
PY - 2026
DA - 2026/10/27
JO - Journal of Insect Science
DO - 10.1093/jisesa/ieag119
UR - https://doi.org/10.1093/jisesa/ieag119
AB - The Asian tiger mosquito, Aedes albopictus (Skuse, 1894) (Diptera: Culicidae), is established and expanding across the Black Sea region, but the redox processes associated with its development and response to insect growth regulators remain poorly understood. We characterized a typical 2-Cys peroxiredoxin (Prx2) by examining intraspecific sequence variation, conserved protein features, enzymatic activity, developmental transcription, and transcription following exposure to 0.06 ppb pyriproxyfen. The coding region was polymorphic among Turkish isolates, but all substitutions were synonymous, and the deduced amino acid sequence was conserved. Catalytic cysteines and hallmark motifs supported classification as a typical 2-Cys peroxiredoxin. Recombinant His-tagged Prx2 migrated at the expected molecular size and removed hydrogen peroxide in a concentration- and thiol-dependent manner. Basal transcript abundance varied across development, reaching its lowest level in pupae and higher levels in adults. Exposure to 0.06 ppb pyriproxyfen was associated with increased Prx2 transcript abundance in L3 and L4 larvae, whereas no statistically detectable treatment-related difference was observed among the surviving pupae or adults at the sampled time points. Together, these findings identify Prx2 as a conserved, functional thiol peroxidase and demonstrate a pyriproxyfen-associated larval transcriptional response under the conditions tested. Because this concentration was not independently validated as sublethal in the present colony and later-stage sampling necessarily comprised survivors of the original exposure, the data do not distinguish developmental attenuation of the response from survivor selection.
SN - 1536-2442
ER -