Genome-Wide Characterization and Heat Responsive Expression Profiling of the HSP10 Co-Chaperonin Gene Family in Brassica juncea.
4 affiliations
- 1School of Breeding and Multiplication (Sanya Nanfan Research Institute of Breeding and Multiplication), College of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.
- 2Guangxi Key Laboratory of Agro-Environment and Agric Products Safety, Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning 530004, China.
- 3Key Laboratory of Salt-Tolerant Crops Biotechnology of Hainan Province, Sanya 572025, China.
- 4International Center for Interdisciplinary Research in Sciences (ICIRS), The University of Lahore, Lahore 54000, Pakistan.
Abstract
Heat stress disrupts protein homeostasis, yet the thermoregulatory roles of HSP10/CPN10 co-chaperonins in polyploid crops remain unexplored. This study conducted the first genome-wide characterization of the HSP10 gene family in allotetraploid Brassica juncea to identify heat tolerance candidates. Genome-wide identification of BjuHSP10 genes was performed across A and B subgenomes, followed by phylogenetic, gene structure, duplication, synteny, promoter, and structural analyses. Transcriptional responses were evaluated under moderate (28/24 °C) and severe (36/32 °C) heat stress conditions. Ten BjuHSP10 genes were identified, encoding six mitochondrial, three chloroplast-targeted, and one cytoplasmic protein. Mitochondrial members were small (~10.7 kDa) with three-exon structures, whereas chloroplast members were larger (15.5-27.1 kDa) with complex exon-intron organization. Segmental duplication drove family expansion (19 duplicated pairs), with differential retention of Arabidopsis homologs. Promoter regions contained 391 putative cis-regulatory elements associated with stress, hormonal, and developmental regulation. Under heat stress, BjuHSP10-4 and BjuHSP10-5 were induced under both treatments; BjuHSP10-1/3/6/9 responded only to severe stress, while four genes were repressed. Structural modeling confirmed conserved β-rich GroES/CPN10-like folds. These findings identify candidate BjuHSP10 genes, particularly BjuHSP10-4 and BjuHSP10-5, as promising targets for heat-tolerance improvement in mustard, providing a foundation for future breeding and functional studies in polyploid crops.
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BibTeX
@article{saeed2026genomewide,
title = {{Genome-Wide Characterization and Heat Responsive Expression Profiling of the HSP10 Co-Chaperonin Gene Family in Brassica juncea.}},
author = {Wajid Saeed and Zhangrong Chen and Samavia Mubeen and Sana Basharat and Qiqi Peng and Huiping Zhao and Muzammal Rehman and Yun Li and Muhammad Waseem and Pingwu Liu},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198640},
url = {https://europepmc.org/article/MED/42883890},
note = {CC BY},
}
RIS
TY - JOUR
TI - Genome-Wide Characterization and Heat Responsive Expression Profiling of the HSP10 Co-Chaperonin Gene Family in Brassica juncea.
AU - Wajid Saeed
AU - Zhangrong Chen
AU - Samavia Mubeen
AU - Sana Basharat
AU - Qiqi Peng
AU - Huiping Zhao
AU - Muzammal Rehman
AU - Yun Li
AU - Muhammad Waseem
AU - Pingwu Liu
PY - 2026
DA - 2026/09/27
JO - International journal of molecular sciences
DO - 10.3390/ijms27198640
UR - https://europepmc.org/article/MED/42883890
AB - Heat stress disrupts protein homeostasis, yet the thermoregulatory roles of HSP10/CPN10 co-chaperonins in polyploid crops remain unexplored. This study conducted the first genome-wide characterization of the HSP10 gene family in allotetraploid Brassica juncea to identify heat tolerance candidates. Genome-wide identification of BjuHSP10 genes was performed across A and B subgenomes, followed by phylogenetic, gene structure, duplication, synteny, promoter, and structural analyses. Transcriptional responses were evaluated under moderate (28/24 °C) and severe (36/32 °C) heat stress conditions. Ten BjuHSP10 genes were identified, encoding six mitochondrial, three chloroplast-targeted, and one cytoplasmic protein. Mitochondrial members were small (~10.7 kDa) with three-exon structures, whereas chloroplast members were larger (15.5-27.1 kDa) with complex exon-intron organization. Segmental duplication drove family expansion (19 duplicated pairs), with differential retention of Arabidopsis homologs. Promoter regions contained 391 putative cis-regulatory elements associated with stress, hormonal, and developmental regulation. Under heat stress, BjuHSP10-4 and BjuHSP10-5 were induced under both treatments; BjuHSP10-1/3/6/9 responded only to severe stress, while four genes were repressed. Structural modeling confirmed conserved β-rich GroES/CPN10-like folds. These findings identify candidate BjuHSP10 genes, particularly BjuHSP10-4 and BjuHSP10-5, as promising targets for heat-tolerance improvement in mustard, providing a foundation for future breeding and functional studies in polyploid crops.
SN - 1422-0067
ER -