Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection
Abstract
The initial steps of the human immunodeficiency virus type 1 (HIV-1) life cycle are regulated by cellular RNA-binding proteins, but only a few have been identified. Here, we developed in virion RNA interactome capture (ivRIC) to comprehensively profile the direct protein interactors of the HIV-1 genomic (g)RNA inside the viral particles. We identified 104 cellular RNA-binding proteins in virions (ivRBPs), many of which are nuclear. We determined the interactome of the viral RBP Rev and discovered that nuclear ivRBPs may associate gRNA in the nucleus and continue bound after the genesis of the viral particles. We also observed that ivRBPs are not incorporated into viral particles based on their abundance, but likely through selective mechanisms. Moreover, we show that the ivRBPs PURA and its homolog PURB control HIV-1 particle infectivity and engage with several viral proteins and key elements within HIV-1 gRNA, showcasing the importance of ivRBPs for HIV-1 infection.
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BibTeX
@article{garciamoreno2026incorporation,
title = {{Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection}},
author = {Manuel Garcia-Moreno and Azman Embarc-Buh and Robin Truman and Marko Noerenberg and Louisa Iselin and Honglin Chen and Caroline E Lenz and Jeffrey Y Lee and Kate Dicker and Snehith Dyavari Shetty and Natasha Palmalux and Quan Gu and Thibault J.M. Sohier and Aino I Järvelin and Wael Kamel and Vincenzo Ruscica and Emiliano P. Ricci and Ilan Davis and Shabaz Mohammed and Alfredo Castello},
journal = {Cell Reports},
year = {2026},
month = oct,
doi = {10.1016/j.celrep.2026.117090},
url = {https://hal.science/hal-05782017v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection
AU - Manuel Garcia-Moreno
AU - Azman Embarc-Buh
AU - Robin Truman
AU - Marko Noerenberg
AU - Louisa Iselin
AU - Honglin Chen
AU - Caroline E Lenz
AU - Jeffrey Y Lee
AU - Kate Dicker
AU - Snehith Dyavari Shetty
AU - Natasha Palmalux
AU - Quan Gu
AU - Thibault J.M. Sohier
AU - Aino I Järvelin
AU - Wael Kamel
AU - Vincenzo Ruscica
AU - Emiliano P. Ricci
AU - Ilan Davis
AU - Shabaz Mohammed
AU - Alfredo Castello
PY - 2026
DA - 2026/10/06
JO - Cell Reports
DO - 10.1016/j.celrep.2026.117090
UR - https://hal.science/hal-05782017v1
AB - The initial steps of the human immunodeficiency virus type 1 (HIV-1) life cycle are regulated by cellular RNA-binding proteins, but only a few have been identified. Here, we developed in virion RNA interactome capture (ivRIC) to comprehensively profile the direct protein interactors of the HIV-1 genomic (g)RNA inside the viral particles. We identified 104 cellular RNA-binding proteins in virions (ivRBPs), many of which are nuclear. We determined the interactome of the viral RBP Rev and discovered that nuclear ivRBPs may associate gRNA in the nucleus and continue bound after the genesis of the viral particles. We also observed that ivRBPs are not incorporated into viral particles based on their abundance, but likely through selective mechanisms. Moreover, we show that the ivRBPs PURA and its homolog PURB control HIV-1 particle infectivity and engage with several viral proteins and key elements within HIV-1 gRNA, showcasing the importance of ivRBPs for HIV-1 infection.
SN - 2211-1247
SN - 2211-1247
ER -