VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus

Abstract

Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are essential for pluripotency acquisition and successful shoot regeneration. Our genetic and transcriptomic analyses revealed that the regulatory roles of VLCFAs on pluripotency acquisition involve inter-cell layer signaling in callus tissue, while they are at least partly independent of ATML1/PDF2 functions and cuticular wax synthesis in the outermost layer. VLCFAs spatially restrict procambium cell identity by non-cell-autonomously suppressing cytokinin signaling, thereby allowing for establishment of the middle-cell layer. We propose that the inhibitory relationships between layer-specific regulators underlie the intricate balance of cellular fate determination in pluripotent callus.

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BibTeX RIS

BibTeX

@misc{doll2026vlcfamediated,
  title = {{VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus}},
  author = {Y. Doll and T. Nobusawa and M. Kojima and K. Nagata and K. Matsuda-Ito and A. P. Mähönen and T. Matsuda and M. Abe and H. Sakakibara and M. Ikeuchi},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.01.748728},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1},
  note = {CC BY},
}

RIS

TY  - UNPB
TI  - VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus
AU  - Y. Doll
AU  - T. Nobusawa
AU  - M. Kojima
AU  - K. Nagata
AU  - K. Matsuda-Ito
AU  - A. P. Mähönen
AU  - T. Matsuda
AU  - M. Abe
AU  - H. Sakakibara
AU  - M. Ikeuchi
PY  - 2026
DA  - 2026/09/01
PB  - bioRxiv
DO  - 10.64898/2026.09.01.748728
UR  - https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1
AB  - Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are essential for pluripotency acquisition and successful shoot regeneration. Our genetic and transcriptomic analyses revealed that the regulatory roles of VLCFAs on pluripotency acquisition involve inter-cell layer signaling in callus tissue, while they are at least partly independent of ATML1/PDF2 functions and cuticular wax synthesis in the outermost layer. VLCFAs spatially restrict procambium cell identity by non-cell-autonomously suppressing cytokinin signaling, thereby allowing for establishment of the middle-cell layer. We propose that the inhibitory relationships between layer-specific regulators underlie the intricate balance of cellular fate determination in pluripotent callus.
ER  -

Abstract

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