Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to in neonatal endothelial cells
- 1Biology Department, Sonoma State University, Rohnert Park, CA 94928, USA
Abstract
Homologous chromosomes are spatially segregated, or antipaired, in neonatal human endothelial cells at metaphase and anaphase, which might help prevent abnormal recombination. However, it is unclear whether this antipairing persists in adult endothelial cells. To test whether the antipairing, or one homolog per nuclear hemisphere motif, is conserved in adult endothelial cells, we examined human aortic endothelial cells at metaphase. Using immunolabeling with DNA fluorescent in situ hybridization (ImmunoFISH) and high-resolution confocal microscopy to visualize the chromosomes and centrosomes, we found that small homologous chromosomes 13, 15, 17, 19, 21, 22, and the sex chromosomes (XY) exhibit a loss of spatial segregation in human adult aortic endothelial cells. In addition, fewer adult endothelial cells showed segregation for the larger chromosomes 1, 4 and XX, as compared to in neonatal endothelial cells. Notably, we observed a higher frequency of abnormal pairing in both small and large chromosomes in adult aortic endothelial cells as compared to in neonatal umbilical vein endothelial cells. These findings suggest that mechanisms governing chromosome antipairing might decline with aortic endothelial cell age.
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BibTeX
@article{morales2026higher,
title = {{Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to in neonatal endothelial cells}},
author = {Jemery Morales and Omar Akhtar and Gabriel Quintero Plancarte and Lisa Hua},
journal = {Journal of Cell Science},
year = {2026},
month = oct,
doi = {10.1242/jcs.264668},
url = {https://doi.org/10.1242/jcs.264668},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to in neonatal endothelial cells
AU - Jemery Morales
AU - Omar Akhtar
AU - Gabriel Quintero Plancarte
AU - Lisa Hua
PY - 2026
DA - 2026/10/23
JO - Journal of Cell Science
DO - 10.1242/jcs.264668
UR - https://doi.org/10.1242/jcs.264668
AB - Homologous chromosomes are spatially segregated, or antipaired, in neonatal human endothelial cells at metaphase and anaphase, which might help prevent abnormal recombination. However, it is unclear whether this antipairing persists in adult endothelial cells. To test whether the antipairing, or one homolog per nuclear hemisphere motif, is conserved in adult endothelial cells, we examined human aortic endothelial cells at metaphase. Using immunolabeling with DNA fluorescent in situ hybridization (ImmunoFISH) and high-resolution confocal microscopy to visualize the chromosomes and centrosomes, we found that small homologous chromosomes 13, 15, 17, 19, 21, 22, and the sex chromosomes (XY) exhibit a loss of spatial segregation in human adult aortic endothelial cells. In addition, fewer adult endothelial cells showed segregation for the larger chromosomes 1, 4 and XX, as compared to in neonatal endothelial cells. Notably, we observed a higher frequency of abnormal pairing in both small and large chromosomes in adult aortic endothelial cells as compared to in neonatal umbilical vein endothelial cells. These findings suggest that mechanisms governing chromosome antipairing might decline with aortic endothelial cell age.
SN - 0021-9533
SN - 1477-9137
ER -