Mammalian condensin I controls higher-order chromosome organization and homologous recombination in meiotic prophase I
- 1Centre de Biologie Structurale (CBS), Univ Montpellier, CNRS, INSERM, 34090 Montpellier, France
Abstract
The meiotic chromosome structure, defined by the axis-chromatin loop organization, is essential for proper homologue synapsis through the formation of the synaptonemal complex, and faithful homologous recombination. Here, we show that the conserved SMC-condensin I complex, which shapes the genome through chromatin loop extrusion, plays a pivotal role in controlling the higher-order prophase I meiotic chromosome structure. By analyzing the NCAPD2 condensin I subunit, we found that this complex colocalizes with prophase I chromatin and is essential for male fertility. NCAPD2 restricts chromatin loop extension and controls the localization of chromosome structural proteins, including the axial component HORMAD1 and central element proteins (SYCP1, TEX12, SIX6OS1), thus regulating the synaptonemal complex width. Moreover, NCAPD2 promotes timely and efficient recombination by controlling {gamma}H2AX, DMC1 and pro-crossover proteins turnover. We propose that condensin I, by organizing chromatin loops in prophase I, controls synaptonemal complex organization and recombination outcome in mammals.
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BibTeX
@misc{toe2026mammalian,
title = {{Mammalian condensin I controls higher-order chromosome organization and homologous recombination in meiotic prophase I}},
author = {L. Dal Toe and B. Diagouraga and A. Nore and A. Bost and E. Grosjean and J. Cau and T. ROBERT},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.15.751738},
url = {https://www.biorxiv.org/content/10.64898/2026.09.15.751738v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Mammalian condensin I controls higher-order chromosome organization and homologous recombination in meiotic prophase I
AU - L. Dal Toe
AU - B. Diagouraga
AU - A. Nore
AU - A. Bost
AU - E. Grosjean
AU - J. Cau
AU - T. ROBERT
PY - 2026
DA - 2026/09/15
PB - bioRxiv
DO - 10.64898/2026.09.15.751738
UR - https://www.biorxiv.org/content/10.64898/2026.09.15.751738v1
AB - The meiotic chromosome structure, defined by the axis-chromatin loop organization, is essential for proper homologue synapsis through the formation of the synaptonemal complex, and faithful homologous recombination. Here, we show that the conserved SMC-condensin I complex, which shapes the genome through chromatin loop extrusion, plays a pivotal role in controlling the higher-order prophase I meiotic chromosome structure. By analyzing the NCAPD2 condensin I subunit, we found that this complex colocalizes with prophase I chromatin and is essential for male fertility. NCAPD2 restricts chromatin loop extension and controls the localization of chromosome structural proteins, including the axial component HORMAD1 and central element proteins (SYCP1, TEX12, SIX6OS1), thus regulating the synaptonemal complex width. Moreover, NCAPD2 promotes timely and efficient recombination by controlling {gamma}H2AX, DMC1 and pro-crossover proteins turnover. We propose that condensin I, by organizing chromatin loops in prophase I, controls synaptonemal complex organization and recombination outcome in mammals.
ER -