A Sensitive LC-MS/MS Method for Quantifying Beta-Casomorphin 5 in Pasteurized Milk.
4 affiliations
- 1Food Science and Nutrition, School of Medical, Molecular and Forensic Sciences Murdoch University Murdoch Western Australia Australia.
- 2Centre for Crop and Food Innovation, Food Futures Institute Murdoch University Murdoch Western Australia Australia.
- 3Bannister Downs Dairy Northcliffe Western Australia Australia.
- 4School of Maths, Statistics, Chemistry and Physics Murdoch University Murdoch Western Australia Australia.
Abstract
This study aimed to apply a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify beta-casomorphin 5 (β-CM5) content in pasteurized milk (PM) and to evaluate the effect of heat treatment on this peptide. Commercial PM samples were collected from local supermarkets, and heat treatment of industrial milk (IP) samples was performed at 62°C and 72°C for 5 min. The milk samples were purified using solid-phase extraction (SPE) and analyzed for β-CM5 using the LC-MS/MS combined with stable isotope-labeled internal standards. The method achieved high sensitivity, with a limit of detection of 0.03 ng/g. The concentration of β-CM5 ranged from 0.10 to 0.55 ng/g for commercial PM samples and from 0.10 to 0.13 ng/g for IP samples. These results suggest that industrial heat treatment had a very slight effect on β-CM5, and variation in this peptide content in PM samples is primarily driven by differences in raw milk origin, such as endogenous enzyme activity and beta-casein content, rather than pasteurization conditions.
Related
- GEODESIC LENGTH MORPHOMETRY: A simple method for quantifying severe degeneration laterally in rodent models of retinal photic injury.
- Pasteurized Akkermansia muciniphila MucT for weight loss maintenance in people with overweight and obesity: a controlled randomized trial
- A sensitive and specific microchimerism detection method using custom designed genotyping and companion qPCR assays
- Knock-Down of Glucocorticoid Receptor Beta (GRβ) Enhances the Ocular Hypertensive Response to Dexamethasone in C57BL/6J Mice
- B Cell Deficiency Protects Against the Development of c‐MET and Beta‐Catenin Driven Hepatocellular Carcinoma in Mice
BibTeX
@article{nguyen2026sensitive,
title = {{A Sensitive LC-MS/MS Method for Quantifying Beta-Casomorphin 5 in Pasteurized Milk.}},
author = {Duc Doan Nguyen and Vicky Solah and Suzanne Daubney and Malgorzata Kowalczyk and Wendy Hunt},
journal = {Food science \& nutrition},
year = {2026},
month = oct,
doi = {10.1002/fsn3.72484},
url = {https://europepmc.org/article/MED/42903250},
note = {CC BY},
}
RIS
TY - JOUR
TI - A Sensitive LC-MS/MS Method for Quantifying Beta-Casomorphin 5 in Pasteurized Milk.
AU - Duc Doan Nguyen
AU - Vicky Solah
AU - Suzanne Daubney
AU - Malgorzata Kowalczyk
AU - Wendy Hunt
PY - 2026
DA - 2026/10/19
JO - Food science & nutrition
DO - 10.1002/fsn3.72484
UR - https://europepmc.org/article/MED/42903250
AB - This study aimed to apply a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify beta-casomorphin 5 (β-CM5) content in pasteurized milk (PM) and to evaluate the effect of heat treatment on this peptide. Commercial PM samples were collected from local supermarkets, and heat treatment of industrial milk (IP) samples was performed at 62°C and 72°C for 5 min. The milk samples were purified using solid-phase extraction (SPE) and analyzed for β-CM5 using the LC-MS/MS combined with stable isotope-labeled internal standards. The method achieved high sensitivity, with a limit of detection of 0.03 ng/g. The concentration of β-CM5 ranged from 0.10 to 0.55 ng/g for commercial PM samples and from 0.10 to 0.13 ng/g for IP samples. These results suggest that industrial heat treatment had a very slight effect on β-CM5, and variation in this peptide content in PM samples is primarily driven by differences in raw milk origin, such as endogenous enzyme activity and beta-casein content, rather than pasteurization conditions.
SN - 2048-7177
ER -