Decoupling Sugar Availability and Metabolic Efficiency Reveals Bottlenecks in Ethanol Production from Lignocellulosic Hydrothermal Hydrolysates
- 1Federal University of Santa Maria , , Roraima Avenue , ,
- 2University of Minho , , Campus de Gualtar , ,
Abstract
Lignocellulosic hydrolysates derived from hydrothermal processing often result in low ethanol yields, which are commonly attributed to inhibitory compounds and suboptimal sugar composition. However, the relative contribution of sugar availability and microbial metabolic efficiency to these limitations remains poorly understood. This study systematically evaluated the ethanol production by Scheffersomyces stipitis using hydrolysates obtained from steam explosion (SE), subcritical water hydrolysis (SWH), enzymatic hydrolysis (EH), and their sequential combinations, under identical fermentation conditions. To decouple process limitations, ethanol yields were analyzed based on both total sugar available and sugar effectively consumed. Results showed that hydrothermal hydrolysates (SE and SWH) led to low ethanol yields (0.08–0.14 g/g), primarily due to limited sugar uptake associated with the presence of inhibitory compounds and low sugar concentrations. In contrast, enzymatic hydrolysates, particularly after SE pretreatment (SE + EH), achieved yields up to 0.50 g/g, approaching the theoretical maximum. Notably, when yields were calculated based on sugar consumed, significantly higher efficiencies were observed across all conditions, revealing that the intrinsic metabolic capacity of S. stipitis remained largely preserved even in inhibitory media. These findings demonstrate that reduced ethanol production in lignocellulosic hydrolysates is predominantly governed by limitations in sugar assimilation rather than metabolic conversion. This study provides a mechanistic framework for distinguishing between substrate accessibility and metabolic efficiency, offering new insights for the optimization of second-generation ethanol processes.
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BibTeX
@article{vezaro2026decoupling,
title = {{Decoupling Sugar Availability and Metabolic Efficiency Reveals Bottlenecks in Ethanol Production from Lignocellulosic Hydrothermal Hydrolysates}},
author = {Francisco D. Vezaro and Alex Schulz and Barbara V. da Rosa and Chrysttian Matheus Schneider Garcia and Raquel C. Kuhn and José A. C. Teixeira and Ederson R. Abaide and Flávio D. Mayer},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c07430},
url = {https://pubs.acs.org/acsodf/article/11/41/63493/5445597/Decoupling-Sugar-Availability-and-Metabolic},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Decoupling Sugar Availability and Metabolic Efficiency Reveals Bottlenecks in Ethanol Production from Lignocellulosic Hydrothermal Hydrolysates
AU - Francisco D. Vezaro
AU - Alex Schulz
AU - Barbara V. da Rosa
AU - Chrysttian Matheus Schneider Garcia
AU - Raquel C. Kuhn
AU - José A. C. Teixeira
AU - Ederson R. Abaide
AU - Flávio D. Mayer
PY - 2026
DA - 2026/10/06
JO - ACS Omega
DO - 10.1021/acsomega.6c07430
UR - https://pubs.acs.org/acsodf/article/11/41/63493/5445597/Decoupling-Sugar-Availability-and-Metabolic
AB - Lignocellulosic hydrolysates derived from hydrothermal processing often result in low ethanol yields, which are commonly attributed to inhibitory compounds and suboptimal sugar composition. However, the relative contribution of sugar availability and microbial metabolic efficiency to these limitations remains poorly understood. This study systematically evaluated the ethanol production by Scheffersomyces stipitis using hydrolysates obtained from steam explosion (SE), subcritical water hydrolysis (SWH), enzymatic hydrolysis (EH), and their sequential combinations, under identical fermentation conditions. To decouple process limitations, ethanol yields were analyzed based on both total sugar available and sugar effectively consumed. Results showed that hydrothermal hydrolysates (SE and SWH) led to low ethanol yields (0.08–0.14 g/g), primarily due to limited sugar uptake associated with the presence of inhibitory compounds and low sugar concentrations. In contrast, enzymatic hydrolysates, particularly after SE pretreatment (SE + EH), achieved yields up to 0.50 g/g, approaching the theoretical maximum. Notably, when yields were calculated based on sugar consumed, significantly higher efficiencies were observed across all conditions, revealing that the intrinsic metabolic capacity of S. stipitis remained largely preserved even in inhibitory media. These findings demonstrate that reduced ethanol production in lignocellulosic hydrolysates is predominantly governed by limitations in sugar assimilation rather than metabolic conversion. This study provides a mechanistic framework for distinguishing between substrate accessibility and metabolic efficiency, offering new insights for the optimization of second-generation ethanol processes.
SN - 2470-1343
ER -