Fungal Enzyme-Assisted Conversion of Water Hyacinth and Sugarcane Bagasse Hemicelluloses into Xylooligosaccharide-Rich Hydrolysates with Potential Prebiotic Applications
4 affiliations
- 1Departamento de Ingeniería Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, Mexico
- 2Departamento de Fundamentos del Conocimiento, Centro Universitario del Norte, Universidad de Guadalajara, Carretera Federal No. 23 Km. 191, Col. Santiago Tlatelolco, Colotlán C.P. 42600, Jalisco, Mexico
- 3Departamento de Ingeniería Biología, Sintética y de Materiales, Centro Universitario de Tlajomulco, Universidad de Guadalajara, Carretera Tlajomulco-Santa Fe Km. 3.5 No. 595, Lomas de Tejeda, Tlajomulco de Zúñiga C.P. 45641, Jalisco, Mexico
- 4Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Gral. Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, Mexico
Abstract
The valorization of lignocellulosic residues through xylooligosaccharides (XOS) production offers a promising route for obtaining value-added compounds from underutilized biomass. In this study, five biocatalysts were produced by solid-state fermentation using water hyacinth (WH) as the substrate and solid support for five fungal strains. Xylanases were extracted from each fermented solid, exhibiting maximum activity between 45–55 °C and pH 5–7. Additionally, the recovery of hemicellulosic fractions from WH and sugarcane bagasse (SCB) reached 14.3 ± 0.91% and 18.4 ± 0.22% of initial dry biomass, respectively. Commercial xylan and hemicellulosic fractions from WH (WHh) and SCB (SCBh), at an initial concentration of 25 g/L, were partially hydrolyzed, releasing 12–18, 9–12 and 2–4 g/L of reducing sugars, respectively, after 6 h of reaction, depending on the enzyme extract used. Xylobiose was detected in all hydrolysates, with the Trichoderma harzianum extract yielding the highest concentrations: 8.3, 4.4, and 2.4 g/L from xylan, WHh, and SCBh, respectively. Hydrolysates supplemented into MRS medium enhanced the growth of Bifidobacterium lactis and Lactococcus lactis compared to the control medium. These findings demonstrate the feasibility of using waste-derived biocatalysts and WH and SCB as inexpensive and abundant sources of hemicellulose to produce XOS-containing hydrolysates with potential prebiotic applications.
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BibTeX
@article{romeroborbon2026fungal,
title = {{Fungal Enzyme-Assisted Conversion of Water Hyacinth and Sugarcane Bagasse Hemicelluloses into Xylooligosaccharide-Rich Hydrolysates with Potential Prebiotic Applications}},
author = {Evelyn Romero-Borbón and Carlos Soltero-Sánchez and Rosa Isela Corona-González and María Ángeles Camacho-Ruiz and Néstor David Ortega-de la Rosa and Jesús Córdova},
journal = {Catalysts},
year = {2026},
month = oct,
doi = {10.3390/catal16110946},
url = {https://www.mdpi.com/2073-4344/16/11/946},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Fungal Enzyme-Assisted Conversion of Water Hyacinth and Sugarcane Bagasse Hemicelluloses into Xylooligosaccharide-Rich Hydrolysates with Potential Prebiotic Applications
AU - Evelyn Romero-Borbón
AU - Carlos Soltero-Sánchez
AU - Rosa Isela Corona-González
AU - María Ángeles Camacho-Ruiz
AU - Néstor David Ortega-de la Rosa
AU - Jesús Córdova
PY - 2026
DA - 2026/10/24
JO - Catalysts
DO - 10.3390/catal16110946
UR - https://www.mdpi.com/2073-4344/16/11/946
AB - The valorization of lignocellulosic residues through xylooligosaccharides (XOS) production offers a promising route for obtaining value-added compounds from underutilized biomass. In this study, five biocatalysts were produced by solid-state fermentation using water hyacinth (WH) as the substrate and solid support for five fungal strains. Xylanases were extracted from each fermented solid, exhibiting maximum activity between 45–55 °C and pH 5–7. Additionally, the recovery of hemicellulosic fractions from WH and sugarcane bagasse (SCB) reached 14.3 ± 0.91% and 18.4 ± 0.22% of initial dry biomass, respectively. Commercial xylan and hemicellulosic fractions from WH (WHh) and SCB (SCBh), at an initial concentration of 25 g/L, were partially hydrolyzed, releasing 12–18, 9–12 and 2–4 g/L of reducing sugars, respectively, after 6 h of reaction, depending on the enzyme extract used. Xylobiose was detected in all hydrolysates, with the Trichoderma harzianum extract yielding the highest concentrations: 8.3, 4.4, and 2.4 g/L from xylan, WHh, and SCBh, respectively. Hydrolysates supplemented into MRS medium enhanced the growth of Bifidobacterium lactis and Lactococcus lactis compared to the control medium. These findings demonstrate the feasibility of using waste-derived biocatalysts and WH and SCB as inexpensive and abundant sources of hemicellulose to produce XOS-containing hydrolysates with potential prebiotic applications.
SN - 2073-4344
ER -