CAN AQUEOUS PROTEOMICS PREDICT THE RECURRENCE OF RHEGMATOGENOUS RETINAL DETACHMENT?
Abstract
PURPOSE: To explore whether the proteome of aqueous collected during primary repair of rhegmatogenous retinal detachment (RD), differs between patients who experience recurrency (Recurrent RD Group) and those who do not (No Recurrent RD Group). METHODS: The aqueous proteome collected during primary surgery of 13 patients undergoing Recurrent RD was compared with 11 age- and sex-matched patients successfully operated for rhegmatogenous RD with no recurrency after 12-month follow-up, regardless of surgical technique. A label-free shotgun proteomics approach identified and quantified the repertoire of aqueous proteins. Differential protein expression between groups was determined using the Limma moderated Bayesian t -test, followed by false discovery rate (FDR) validation using Storey q-test. RESULTS: Aqueous profiling identified >800 unique proteins; 45 exclusive to the Recurrent RD group, 10 exclusive to the no Recurrent RD group, and 33 differently expressed between groups (log2fold-change≥∣0.57∣, FDR ≤ 0.05). Proteins upregulated in Recurrent RD patients, clearly pointed to mechanisms of cell:cell and cell:matrix adhesiveness and mechano-transduction signaling pathways. Upregulated proteins included extracellular matrix components such as type I and IV collagens, biglycan, proteoglycans, and cell-membrane adhesion molecules. CONCLUSION: The baseline aqueous composition of RD patients that will eventually develop recurrency, differs significantly from those who will not, and already contains molecular signatures that may help identify the risk of recurrency at the time of primary repair. While acknowledging the pilot nature of the study, findings of this study strongly suggest that Recurrent RD is associated with cell adhesiveness pathways early alterations, offering targets for prognostic assessment and therapy.
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BibTeX
@article{zingale2026aqueous,
title = {{CAN AQUEOUS PROTEOMICS PREDICT THE RECURRENCE OF RHEGMATOGENOUS RETINAL DETACHMENT?}},
author = {Gabriele Antonio Zingale and Sara Giammaria and Irene Pandino and Luca Placentino and Guido Ripandelli and Giuseppe Grasso and David Steel and Mario Romano and Diego Sbardella and Tommaso Rossi},
journal = {RETINA. The Journal of Retinal and Vitreous Diseases},
year = {2026},
month = oct,
doi = {10.1097/iae.0000000000004777},
url = {https://hal.science/hal-05782865v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - CAN AQUEOUS PROTEOMICS PREDICT THE RECURRENCE OF RHEGMATOGENOUS RETINAL DETACHMENT?
AU - Gabriele Antonio Zingale
AU - Sara Giammaria
AU - Irene Pandino
AU - Luca Placentino
AU - Guido Ripandelli
AU - Giuseppe Grasso
AU - David Steel
AU - Mario Romano
AU - Diego Sbardella
AU - Tommaso Rossi
PY - 2026
DA - 2026/10/07
JO - RETINA. The Journal of Retinal and Vitreous Diseases
DO - 10.1097/iae.0000000000004777
UR - https://hal.science/hal-05782865v1
AB - PURPOSE: To explore whether the proteome of aqueous collected during primary repair of rhegmatogenous retinal detachment (RD), differs between patients who experience recurrency (Recurrent RD Group) and those who do not (No Recurrent RD Group). METHODS: The aqueous proteome collected during primary surgery of 13 patients undergoing Recurrent RD was compared with 11 age- and sex-matched patients successfully operated for rhegmatogenous RD with no recurrency after 12-month follow-up, regardless of surgical technique. A label-free shotgun proteomics approach identified and quantified the repertoire of aqueous proteins. Differential protein expression between groups was determined using the Limma moderated Bayesian t -test, followed by false discovery rate (FDR) validation using Storey q-test. RESULTS: Aqueous profiling identified >800 unique proteins; 45 exclusive to the Recurrent RD group, 10 exclusive to the no Recurrent RD group, and 33 differently expressed between groups (log2fold-change≥∣0.57∣, FDR ≤ 0.05). Proteins upregulated in Recurrent RD patients, clearly pointed to mechanisms of cell:cell and cell:matrix adhesiveness and mechano-transduction signaling pathways. Upregulated proteins included extracellular matrix components such as type I and IV collagens, biglycan, proteoglycans, and cell-membrane adhesion molecules. CONCLUSION: The baseline aqueous composition of RD patients that will eventually develop recurrency, differs significantly from those who will not, and already contains molecular signatures that may help identify the risk of recurrency at the time of primary repair. While acknowledging the pilot nature of the study, findings of this study strongly suggest that Recurrent RD is associated with cell adhesiveness pathways early alterations, offering targets for prognostic assessment and therapy.
SN - 0275-004X
SN - 1539-2864
ER -