A chromosome-level genome assembly of the classical model nematode Panagrellus redivivus for comparative studies of parasitism evolution
- 1The University of Tokyo
Abstract
Panagrellus redivivus is a free-living nematode that served as an early model system for nematode biology prior to Caenorhabditis elegans. As a member of Clade IV, a clade harboring major plant-parasitic (e.g., Bursaphelenchus xylophilus) and animal-parasitic (e.g., Strongyloides spp.) species, P. redivivus represents a crucial, phylogenetically proximate non-parasitic reference for investigating the evolution of parasitism. Here, we present a chromosome-level genome assembly of P. redivivus constructed using Oxford Nanopore long reads, Illumina short reads, and Hi-C scaffolding. The assembly is resolved into five chromosome-scale scaffolds, with completeness validated by Hi-C contact maps and the identification of a non-canonical telomeric repeat motif. The assembly demonstrates high gene-space completeness (97.1% BUSCO) and contains 17,839 predicted protein-coding genes. Comparative orthology analysis revealed extensive orthogroup sharing between P. redivivus and Clade IV parasites, enabling comparative evolutionary studies with reduced confounding effects from deep lineage divergence. This high-quality genome provides a key resource for investigating the genetic basis of distinctive biological traits, including ovoviviparity, as well as gene family dynamics and parasite evolution.
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BibTeX
@misc{sato2026chromosomelevel,
title = {{A chromosome-level genome assembly of the classical model nematode Panagrellus redivivus for comparative studies of parasitism evolution}},
author = {K. Sato and S. Sun and T. Yamashita and A. Yoshida and R. Shinya and P. W. Sternberg and T. Kikuchi},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.22.761182},
url = {https://www.biorxiv.org/content/10.64898/2026.10.22.761182v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - A chromosome-level genome assembly of the classical model nematode Panagrellus redivivus for comparative studies of parasitism evolution
AU - K. Sato
AU - S. Sun
AU - T. Yamashita
AU - A. Yoshida
AU - R. Shinya
AU - P. W. Sternberg
AU - T. Kikuchi
PY - 2026
DA - 2026/10/22
PB - bioRxiv
DO - 10.64898/2026.10.22.761182
UR - https://www.biorxiv.org/content/10.64898/2026.10.22.761182v1
AB - Panagrellus redivivus is a free-living nematode that served as an early model system for nematode biology prior to Caenorhabditis elegans. As a member of Clade IV, a clade harboring major plant-parasitic (e.g., Bursaphelenchus xylophilus) and animal-parasitic (e.g., Strongyloides spp.) species, P. redivivus represents a crucial, phylogenetically proximate non-parasitic reference for investigating the evolution of parasitism. Here, we present a chromosome-level genome assembly of P. redivivus constructed using Oxford Nanopore long reads, Illumina short reads, and Hi-C scaffolding. The assembly is resolved into five chromosome-scale scaffolds, with completeness validated by Hi-C contact maps and the identification of a non-canonical telomeric repeat motif. The assembly demonstrates high gene-space completeness (97.1% BUSCO) and contains 17,839 predicted protein-coding genes. Comparative orthology analysis revealed extensive orthogroup sharing between P. redivivus and Clade IV parasites, enabling comparative evolutionary studies with reduced confounding effects from deep lineage divergence. This high-quality genome provides a key resource for investigating the genetic basis of distinctive biological traits, including ovoviviparity, as well as gene family dynamics and parasite evolution.
ER -