Evidence for strong interplay between the nucleotide and base excision repair pathways in D. radiodurans
Abstract
Deinococcus radiodurans harbors a largely classical bacterial DNA repair machinery yet displays exceptional resistance to ultra-violet and ionizing radiation. To investigate whether crosstalk between its DNA repair pathways contributes to this phenotype, we mapped putative interactions between the nucleotide excision repair (NER) and base excision repair (BER) pathways, which together are responsible for the removal of nucleobase lesions. Using a bacterial two-hybrid system, we identified multiple direct interactions between NER and BER proteins, notably involving the two UvrA variants, and validated these interactions in vitro . Furthermore, functional analyses revealed that NER interferes with the BER-mediated removal of oxidized guanines by the Fpg DNA glycosylase, likely through competition for DNA binding and sequestration of Fpg. Finally, UvrB and UvrC were found to further process the Fpg incision product in an ATP-dependent, UvrA1-independent manner. Together, these results demonstrate a multi-level crosstalk between NER and BER in D. radiodurans , which may contribute to its extraordinary DNA repair capacity. To our knowledge, this represents the first evidence of such a complex interplay in bacteria.
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BibTeX
@misc{hayek2026evidence,
title = {{Evidence for strong interplay between the nucleotide and base excision repair pathways in D. radiodurans}},
author = {Mohammad Rida Hayek and Salvatore de Bonis and Christine Saint-Pierre and Jean-Baptiste Reiser and Elin Moe and Jean-Luc Ravanat and Joanna Timmins},
howpublished = {HAL},
year = {2026},
month = jun,
doi = {10.64898/2026.06.04.729788},
url = {https://hal.science/hal-05847935v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - UNPB
TI - Evidence for strong interplay between the nucleotide and base excision repair pathways in D. radiodurans
AU - Mohammad Rida Hayek
AU - Salvatore de Bonis
AU - Christine Saint-Pierre
AU - Jean-Baptiste Reiser
AU - Elin Moe
AU - Jean-Luc Ravanat
AU - Joanna Timmins
PY - 2026
DA - 2026/06/09
PB - HAL
DO - 10.64898/2026.06.04.729788
UR - https://hal.science/hal-05847935v1
AB - Deinococcus radiodurans harbors a largely classical bacterial DNA repair machinery yet displays exceptional resistance to ultra-violet and ionizing radiation. To investigate whether crosstalk between its DNA repair pathways contributes to this phenotype, we mapped putative interactions between the nucleotide excision repair (NER) and base excision repair (BER) pathways, which together are responsible for the removal of nucleobase lesions. Using a bacterial two-hybrid system, we identified multiple direct interactions between NER and BER proteins, notably involving the two UvrA variants, and validated these interactions in vitro . Furthermore, functional analyses revealed that NER interferes with the BER-mediated removal of oxidized guanines by the Fpg DNA glycosylase, likely through competition for DNA binding and sequestration of Fpg. Finally, UvrB and UvrC were found to further process the Fpg incision product in an ATP-dependent, UvrA1-independent manner. Together, these results demonstrate a multi-level crosstalk between NER and BER in D. radiodurans , which may contribute to its extraordinary DNA repair capacity. To our knowledge, this represents the first evidence of such a complex interplay in bacteria.
ER -