Bioprocessing of Talaromyces purpurogenus for production of dihydromonascorubrin with a powerful antioxidant and antimicrobial activities integrated with the network-guided target prediction and molecular docking analyses.
8 affiliations
- 1Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt. [email protected].
- 2Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
- 3Drug Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
- 4Botany and Microbiology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
- 5Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
- 6Department of Industrial Biotechnology, Faculty of Biotechnology, University of Sadat City, Sadat City, Egypt.
- 7Department of Microbiology, Faculty of Applied Health Sciences Technology, Horus University New Damietta, New Damietta, Egypt.
- 8Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
Abstract
The antibiotic resistance of pathogenic microorganisms due to the irresponsible use in healthcare and agricultural practices is one of the global threats for the human, especially immune-compromised patients. Thus, searching for a novel compounds from fungi with unique affinity to combat the drug-resistant microorganisms was the objective. Talaromyces purpurogenus # PZ292970.1, an endophyte of palm tree Elaeis guineensis, has the highest productivity of azaphilone pigment (25.5 AU410/ml), with a strong activity against E. coli (MIC value 6.8 µg/ml), S. aureus and C. albicans (MIC value 12.5 µg/ml). The extracted pigment of T. purpurogenus has a strong antibiofilm activity for S. aureus (86.2%), C. albicans (85.8%) and E. coli (79.7%), compared to the control. The TLC purified-pigment of T. purpurogenus had a maximum absorbance at λ₄₁₀ nm, with a small one at λ₅₁₀ nm, revealing the identity of the major peak as orange azaphilone pigment. The purified pigment gave a distinct single peak by HPLC, ensures their structural homogeneity. From the FT-IR analyses, the absorption and stretching vibration of the target compound functional groups ensures their spectral identity as polyketide azaphilone. The 1HNMR and 13CNMR spectra revealed characteristic proton signals for the aromatic polyketide related to dihydromonascorubrin derivatives. From LC-MS/MS, the purified compound had 385.2 m/z that were matched to the molecular mass of dihydromonascorubrin, compared the authentic dihydromonascorubrin (382.4 m/z) as suggest from the fragmentation pattern by CFM-ID software. The yield of azaphilone pigment was increased by 2 folds upon bioprocessing by Plackett-Burman design, and by 2.4 folds by γ-irradiation at 1.0 kGy. The pigment showed a powerful antioxidant activity with free radical scavenging and reducing properties as reported by DPPH, ABT and FRAP assays. Integrating target prediction and molecular docking in silico analyses, dihydromonascorubrin exhibited multi-target mechanisms linked to efflux-associated resistance in E. coli and peptidoglycan remodeling in S. aureus.
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BibTeX
@article{elsayed2026bioprocessing,
title = {{Bioprocessing of Talaromyces purpurogenus for production of dihydromonascorubrin with a powerful antioxidant and antimicrobial activities integrated with the network-guided target prediction and molecular docking analyses.}},
author = {Ashraf S A El-Sayed and Samar A Khashana and Sobhy S Abdel-Fatah and Abeer Eldeghidy and Nouran A Abd El-Hady and Ashraf F El-Baz and Manal A El-Shal and Eman Fikry and Rawia A Zayed and Wafaa M Ismaeil},
journal = {BMC microbiology},
year = {2026},
month = oct,
doi = {10.1186/s12866-026-05707-z},
url = {https://europepmc.org/article/MED/42907759},
note = {CC BY},
}
RIS
TY - JOUR
TI - Bioprocessing of Talaromyces purpurogenus for production of dihydromonascorubrin with a powerful antioxidant and antimicrobial activities integrated with the network-guided target prediction and molecular docking analyses.
AU - Ashraf S A El-Sayed
AU - Samar A Khashana
AU - Sobhy S Abdel-Fatah
AU - Abeer Eldeghidy
AU - Nouran A Abd El-Hady
AU - Ashraf F El-Baz
AU - Manal A El-Shal
AU - Eman Fikry
AU - Rawia A Zayed
AU - Wafaa M Ismaeil
PY - 2026
DA - 2026/10/22
JO - BMC microbiology
DO - 10.1186/s12866-026-05707-z
UR - https://europepmc.org/article/MED/42907759
AB - The antibiotic resistance of pathogenic microorganisms due to the irresponsible use in healthcare and agricultural practices is one of the global threats for the human, especially immune-compromised patients. Thus, searching for a novel compounds from fungi with unique affinity to combat the drug-resistant microorganisms was the objective. Talaromyces purpurogenus # PZ292970.1, an endophyte of palm tree Elaeis guineensis, has the highest productivity of azaphilone pigment (25.5 AU410/ml), with a strong activity against E. coli (MIC value 6.8 µg/ml), S. aureus and C. albicans (MIC value 12.5 µg/ml). The extracted pigment of T. purpurogenus has a strong antibiofilm activity for S. aureus (86.2%), C. albicans (85.8%) and E. coli (79.7%), compared to the control. The TLC purified-pigment of T. purpurogenus had a maximum absorbance at λ₄₁₀ nm, with a small one at λ₅₁₀ nm, revealing the identity of the major peak as orange azaphilone pigment. The purified pigment gave a distinct single peak by HPLC, ensures their structural homogeneity. From the FT-IR analyses, the absorption and stretching vibration of the target compound functional groups ensures their spectral identity as polyketide azaphilone. The 1HNMR and 13CNMR spectra revealed characteristic proton signals for the aromatic polyketide related to dihydromonascorubrin derivatives. From LC-MS/MS, the purified compound had 385.2 m/z that were matched to the molecular mass of dihydromonascorubrin, compared the authentic dihydromonascorubrin (382.4 m/z) as suggest from the fragmentation pattern by CFM-ID software. The yield of azaphilone pigment was increased by 2 folds upon bioprocessing by Plackett-Burman design, and by 2.4 folds by γ-irradiation at 1.0 kGy. The pigment showed a powerful antioxidant activity with free radical scavenging and reducing properties as reported by DPPH, ABT and FRAP assays. Integrating target prediction and molecular docking in silico analyses, dihydromonascorubrin exhibited multi-target mechanisms linked to efflux-associated resistance in E. coli and peptidoglycan remodeling in S. aureus.
SN - 1471-2180
ER -