Microchimerism in the human brain, quantitative assessment and single nuclei profiling establish cell types and diversity
- 1Fred Hutchinson Cancer Center
Abstract
Bi-directional maternal-fetal exchange during pregnancy creates a long-term microchimerism legacy in both individuals, but its presence and cellular fate in the human brain remain poorly defined. We identified maternal microchimerism in the majority of surgically resected epilepsy brains by polymorphism-specific qPCR, across temporal, frontal, parietal, and hippocampal regions. We conducted single nucleus RNA sequencing (snRNA-seq) and analysis by cellector, a genetic demultiplexing tool to detect rare allogeneic cells, to test epilepsy specimens and healthy brains from gestation to old age from publicly available snRNA-seq datasets. Microchimerism was detected in both cohorts and revealed diverse neuronal, glial, immune, and vascular identities. L2/3 intratelencephalic neuronal subtypes and microglial/perivascular macrophage were dominant profiles. Microchimerism showed heterogeneous transcriptional profiles and differences from host nuclei. Microchimerism persisted into advanced age but with diminishing frequency. Together, these findings establish microchimerism with cellular diversity as a common feature of the human brain, prenatal through advanced age.
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BibTeX
@misc{kanaan2026microchimerism,
title = {{Microchimerism in the human brain, quantitative assessment and single nuclei profiling establish cell types and diversity}},
author = {S. B. Kanaan and A. McDonough and C. Gentil and J. Ojemann and H. Heaton and R. Behboudi and D. T. A. Eisenberg and S. N. Furlan and D. E. Geraghty and J. Rutledge and F. Urselli and J. R. Weinstein and J. L. Nelson},
howpublished = {bioRxiv},
year = {2026},
month = jun,
doi = {10.64898/2026.06.05.730225},
url = {https://www.biorxiv.org/content/10.64898/2026.06.05.730225v2},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Microchimerism in the human brain, quantitative assessment and single nuclei profiling establish cell types and diversity
AU - S. B. Kanaan
AU - A. McDonough
AU - C. Gentil
AU - J. Ojemann
AU - H. Heaton
AU - R. Behboudi
AU - D. T. A. Eisenberg
AU - S. N. Furlan
AU - D. E. Geraghty
AU - J. Rutledge
AU - F. Urselli
AU - J. R. Weinstein
AU - J. L. Nelson
PY - 2026
DA - 2026/06/05
PB - bioRxiv
DO - 10.64898/2026.06.05.730225
UR - https://www.biorxiv.org/content/10.64898/2026.06.05.730225v2
AB - Bi-directional maternal-fetal exchange during pregnancy creates a long-term microchimerism legacy in both individuals, but its presence and cellular fate in the human brain remain poorly defined. We identified maternal microchimerism in the majority of surgically resected epilepsy brains by polymorphism-specific qPCR, across temporal, frontal, parietal, and hippocampal regions. We conducted single nucleus RNA sequencing (snRNA-seq) and analysis by cellector, a genetic demultiplexing tool to detect rare allogeneic cells, to test epilepsy specimens and healthy brains from gestation to old age from publicly available snRNA-seq datasets. Microchimerism was detected in both cohorts and revealed diverse neuronal, glial, immune, and vascular identities. L2/3 intratelencephalic neuronal subtypes and microglial/perivascular macrophage were dominant profiles. Microchimerism showed heterogeneous transcriptional profiles and differences from host nuclei. Microchimerism persisted into advanced age but with diminishing frequency. Together, these findings establish microchimerism with cellular diversity as a common feature of the human brain, prenatal through advanced age.
ER -