Phylogenetic Analysis of the Most Common Chicken Coccidia, Eimeria acervulina, Isolated From Commercial Poultry Farms in Bangladesh
- 1Department of Parasitology Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh
- 2Department of Biomedical Sciences and Molecular Biology College of Medicine and Dentistry James Cook University Townsville Queensland Australia
- 3Department of Para‐Clinical Courses, Faculty of Veterinary and Animal Sciences Gono Bishwabidyalay Savar Dhaka Bangladesh
Abstract
Background: Eimeria acervulina is the predominant species of coccidia infecting chickens worldwide, leading to significant production losses. The study explores molecular detection and phylogenetic relationships of E. acervulina in poultry farms of Bangladesh.
Methods: A total of 206 microscopically positive faecal samples were processed for PCR amplification. Oocysts were isolated using flotation techniques following microscopic examination. After recovering the genomic DNA, PCR amplification was performed using species‐specific primers focusing on the internal transcribed spacer‐1 (ITS‐1) region. A subset of 36 E. acervulina‐positive samples was randomly sequenced and analysed for genetic diversity and phylogenetic relationships.
Results: A total of 198 (96.11%) E. acervulina samples with a specific 321‐bp amplicon were detected. Sequence analysis identified 15 distinct genotypes and seven single‐nucleotide substitutions, indicating intra‐species genetic variation. Nucleotide diversity (0.00717) and genotype diversity (0.911) suggested a genetically diverse population with closely related variants. The studied genotypes of E. acervulina formed a cluster with the previously identified reference sequence retrieved from GenBank with strong bootstrap support (98%). Similarly, other Eimeria species formed distinct clades independent of geographic origin. Among the studied sequences, two major clusters were observed, with no clear regional structuring.
Conclusion: This study provides the first molecular characterization of E. acervulina from commercial poultry farms in Bangladesh. The findings reveal measurable genetic diversity of E. acervulina circulating in Bangladesh, providing baseline data for epidemiological understanding and control strategies for avian coccidiosis.
Related
- Draft genome assemblies of three Ligilactobacillus salivarius strains isolated from commercial layer hen litter
- Emergence of Novel Highly Pathogenic Avian Influenza Virus A(H5N1) Clade 2.3.4.4b in Commercial Poultry, Bangladesh
- In silico codon optimization and phylogenetic analysis of various strains of Sapovirus
- Beyond the Plastisphere: Phylogenetic and Biogeographic Diversity of Marine Microorganisms with Putative Plastic-Degradation Potential
- Codon bias analysis in the chloroplast genome of Warburgia ugandensis (Canellaceae) and phylogenetic analysis within magnoliids
BibTeX
@article{karmakar2026phylogenetic,
title = {{Phylogenetic Analysis of the Most Common Chicken Coccidia, Eimeria acervulina, Isolated From Commercial Poultry Farms in Bangladesh}},
author = {Bimal Chandra Karmakar and Nusrat Nowrin Shohana and Subir Sarker and Anita Rani Dey and Anisuzzaman and Sharmin Aqter Rony and Shanaz Parvin and Saykat Kumar Debnath and Shirin Akter and Mohammad Zahangir Alam},
journal = {Veterinary Medicine and Science},
year = {2026},
month = nov,
doi = {10.1002/vms3.71309},
url = {https://doi.org/10.1002/vms3.71309},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Phylogenetic Analysis of the Most Common Chicken Coccidia, Eimeria acervulina, Isolated From Commercial Poultry Farms in Bangladesh
AU - Bimal Chandra Karmakar
AU - Nusrat Nowrin Shohana
AU - Subir Sarker
AU - Anita Rani Dey
AU - Anisuzzaman
AU - Sharmin Aqter Rony
AU - Shanaz Parvin
AU - Saykat Kumar Debnath
AU - Shirin Akter
AU - Mohammad Zahangir Alam
PY - 2026
DA - 2026/11/03
JO - Veterinary Medicine and Science
DO - 10.1002/vms3.71309
UR - https://doi.org/10.1002/vms3.71309
AB - Background: Eimeria acervulina is the predominant species of coccidia infecting chickens worldwide, leading to significant production losses. The study explores molecular detection and phylogenetic relationships of E. acervulina in poultry farms of Bangladesh. Methods: A total of 206 microscopically positive faecal samples were processed for PCR amplification. Oocysts were isolated using flotation techniques following microscopic examination. After recovering the genomic DNA, PCR amplification was performed using species‐specific primers focusing on the internal transcribed spacer‐1 (ITS‐1) region. A subset of 36 E. acervulina‐positive samples was randomly sequenced and analysed for genetic diversity and phylogenetic relationships. Results: A total of 198 (96.11%) E. acervulina samples with a specific 321‐bp amplicon were detected. Sequence analysis identified 15 distinct genotypes and seven single‐nucleotide substitutions, indicating intra‐species genetic variation. Nucleotide diversity (0.00717) and genotype diversity (0.911) suggested a genetically diverse population with closely related variants. The studied genotypes of E. acervulina formed a cluster with the previously identified reference sequence retrieved from GenBank with strong bootstrap support (98%). Similarly, other Eimeria species formed distinct clades independent of geographic origin. Among the studied sequences, two major clusters were observed, with no clear regional structuring. Conclusion: This study provides the first molecular characterization of E. acervulina from commercial poultry farms in Bangladesh. The findings reveal measurable genetic diversity of E. acervulina circulating in Bangladesh, providing baseline data for epidemiological understanding and control strategies for avian coccidiosis.
SN - 2053-1095
ER -