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Peer-reviewedCC BY-NC-SA 4.0The Journal of Poultry Science

Effects of Cyclic High Ambient Temperature on Tricarboxylic Acid Cycle Intermediates and Orotate Levels in the Liver and Breast Muscle of Broiler Chickens

  • Ayumi Katafuchi1
  • Saki Shimamoto1
  • Shozo Tomonaga2
  • Miyu Kamimura3
  • Shinya Ishihara4
  • Akira Ohtsuka1
  • Daichi Ijiri1
4 affiliations
  1. 1United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
  2. 2Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
  3. 3South Kyushu Livestock Veterinary Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
  4. 4Graduate School of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan
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Abstract

Heat stress alters tricarboxylic acid (TCA) cycle metabolism in broiler chickens; however, quantitative changes in TCA cycle-related metabolites remain poorly understood. This study determined the effects of heat exposure on TCA cycle intermediate (citrate, isocitrate, α-ketoglutarate, succinate, fumarate, and malate) and orotate levels in the liver and breast muscle of broiler chickens. Twelve 14-day-old male broiler chickens were assigned to either a control group (25 ± 1 °C) or a cyclic high ambient temperature group (35 ± 1 °C for 8 h/day) and reared under these conditions for 4 days. Heat exposure increased liver levels of citrate, α-ketoglutarate, fumarate, and malate, while decreasing succinate and orotate. Heat exposure also decreased the mRNA expression of isocitrate dehydrogenase 3A (IDH3A) and oxoglutarate dehydrogenase, key TCA cycle regulatory enzymes, as well as dihydroorotate dehydrogenase and ubiquinol–cytochrome c reductase Rieske iron–sulfur protein 1 (UQCRFS1), which are involved in mitochondrial electron transport. Heat exposure increased breast muscle levels of succinate, fumarate, and malate, while reducing IDH3A and UQCRFS1 mRNA expression. These findings suggest that heat exposure impairs TCA cycle metabolism in broiler chickens, possibly by reducing the utilization of TCA cycle intermediates through impaired electron transfer in mitochondrial complex III.

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Cite

BibTeXRIS
BibTeX
@article{katafuchi2026effects,
  title = {{Effects of Cyclic High Ambient Temperature on Tricarboxylic Acid Cycle Intermediates and Orotate Levels in the Liver and Breast Muscle of Broiler Chickens}},
  author = {Ayumi Katafuchi and Saki Shimamoto and Shozo Tomonaga and Miyu Kamimura and Shinya Ishihara and Akira Ohtsuka and Daichi Ijiri},
  journal = {The Journal of Poultry Science},
  year = {2026},
  month = oct,
  doi = {10.2141/jpsa.2026024},
  url = {https://doi.org/10.2141/jpsa.2026024},
  note = {CC BY-NC-SA 4.0},
}
RIS
TY  - JOUR
TI  - Effects of Cyclic High Ambient Temperature on Tricarboxylic Acid Cycle Intermediates and Orotate Levels in the Liver and Breast Muscle of Broiler Chickens
AU  - Ayumi Katafuchi
AU  - Saki Shimamoto
AU  - Shozo Tomonaga
AU  - Miyu Kamimura
AU  - Shinya Ishihara
AU  - Akira Ohtsuka
AU  - Daichi Ijiri
PY  - 2026
DA  - 2026/10/28
JO  - The Journal of Poultry Science
DO  - 10.2141/jpsa.2026024
UR  - https://doi.org/10.2141/jpsa.2026024
AB  - Heat stress alters tricarboxylic acid (TCA) cycle metabolism in broiler chickens; however, quantitative changes in TCA cycle-related metabolites remain poorly understood. This study determined the effects of heat exposure on TCA cycle intermediate (citrate, isocitrate, α-ketoglutarate, succinate, fumarate, and malate) and orotate levels in the liver and breast muscle of broiler chickens. Twelve 14-day-old male broiler chickens were assigned to either a control group (25 ± 1 °C) or a cyclic high ambient temperature group (35 ± 1 °C for 8 h/day) and reared under these conditions for 4 days. Heat exposure increased liver levels of citrate, α-ketoglutarate, fumarate, and malate, while decreasing succinate and orotate. Heat exposure also decreased the mRNA expression of isocitrate dehydrogenase 3A (IDH3A) and oxoglutarate dehydrogenase, key TCA cycle regulatory enzymes, as well as dihydroorotate dehydrogenase and ubiquinol–cytochrome c reductase Rieske iron–sulfur protein 1 (UQCRFS1), which are involved in mitochondrial electron transport. Heat exposure increased breast muscle levels of succinate, fumarate, and malate, while reducing IDH3A and UQCRFS1 mRNA expression. These findings suggest that heat exposure impairs TCA cycle metabolism in broiler chickens, possibly by reducing the utilization of TCA cycle intermediates through impaired electron transfer in mitochondrial complex III.
SN  - 1346-7395
SN  - 1349-0486
ER  -