Postharvest senescence of broccoli in the multi-omics era: molecular mechanisms, regulatory networks, and prospects for germplasm innovation
Abstract
Broccoli (Brassica oleracea var. italica) heads are harvested as immature inflorescences whose metabolism remains vigorous after detachment, making postharvest senescence and floret yellowing the leading cause of quality loss and economic devaluation. Over the past decade, the molecular dissection of broccoli senescence has progressed from single-gene expression studies to integrated multi-omics atlases spanning transcriptomes, small RNA and degradome libraries, quantitative proteomes, and metabolomes. This review synthesizes the recent literature along three axes. First, we revisit the chlorophyll degradation pathway as it operates in harvested broccoli florets (from chlorophyll b reductase (BoNYC1) and pheophytinase (BoPPH) to pheophorbide a oxygenase (BoPaO), RCC reductase (BoRCCR), and the STAY-GREEN (BoSGR) master switch) and we highlight how the visible yellow phenotype additionally requires carotenoid retention and biosynthesis. Second, we organize the regulatory architecture now emerging from omics studies. Its hormonal tier is an ethylene–auxin–jasmonate–abscisic acid axis that promotes senescence and is antagonized by cytokinins, brassinosteroids, melatonin, hydrogen sulfide, and the peptide signal CLE14. Its transcriptional tier is dominated by NAC, WRKY, MYB, bHLH, ERF, and TCP factors, exemplified by the BoNAC52–BoRBOHF module that couples hormone signals to reactive oxygen species (ROS) homeostasis. A third tier is post-transcriptional: miRNAs modulate transcript abundance to fine-tune peroxidase activity, chlorophyll biosynthesis, and ROS balance. Third, we connect mechanism to application. We map how preservation technologies (1-MCP, controlled atmospheres, UV and LED irradiation, melatonin, and CLE14) converge on defined molecular nodes. We then ask how the new genomic resources of B. oleracea — the chromosome-scale broccoli reference genome and the 27-genome pan-genome — and CRISPR/Cas9 editing, now demonstrated in broccoli, provide a realistic route toward stay-green, slow-senescing cultivars. We close by identifying six priority frontiers: cell-type-resolved atlases, epigenomic time courses, standardized multi-omics protocols, GWAS for shelf life, rationally designed editing targets, and network-level validation. Broccoli thus illustrates a general arc of postharvest biology, moving from physiology to networks and from networks to designed genotypes.
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BibTeX
@article{jiang2026postharvest,
title = {{Postharvest senescence of broccoli in the multi-omics era: molecular mechanisms, regulatory networks, and prospects for germplasm innovation}},
author = {Han-Min Jiang and Li-Li Liu and Zheng-Hua Wen and Guo-Bao Niu and Xiao-Li Zhang and Xing-Wei Yao and Xiao-Zheng Shan and De-Ling Sun},
journal = {Frontiers in Plant Science},
year = {2026},
month = nov,
doi = {10.3389/fpls.2026.1985976},
url = {https://www.frontiersin.org/articles/10.3389/fpls.2026.1985976},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Postharvest senescence of broccoli in the multi-omics era: molecular mechanisms, regulatory networks, and prospects for germplasm innovation
AU - Han-Min Jiang
AU - Li-Li Liu
AU - Zheng-Hua Wen
AU - Guo-Bao Niu
AU - Xiao-Li Zhang
AU - Xing-Wei Yao
AU - Xiao-Zheng Shan
AU - De-Ling Sun
PY - 2026
DA - 2026/11/06
JO - Frontiers in Plant Science
DO - 10.3389/fpls.2026.1985976
UR - https://www.frontiersin.org/articles/10.3389/fpls.2026.1985976
AB - Broccoli (Brassica oleracea var. italica) heads are harvested as immature inflorescences whose metabolism remains vigorous after detachment, making postharvest senescence and floret yellowing the leading cause of quality loss and economic devaluation. Over the past decade, the molecular dissection of broccoli senescence has progressed from single-gene expression studies to integrated multi-omics atlases spanning transcriptomes, small RNA and degradome libraries, quantitative proteomes, and metabolomes. This review synthesizes the recent literature along three axes. First, we revisit the chlorophyll degradation pathway as it operates in harvested broccoli florets (from chlorophyll b reductase (BoNYC1) and pheophytinase (BoPPH) to pheophorbide a oxygenase (BoPaO), RCC reductase (BoRCCR), and the STAY-GREEN (BoSGR) master switch) and we highlight how the visible yellow phenotype additionally requires carotenoid retention and biosynthesis. Second, we organize the regulatory architecture now emerging from omics studies. Its hormonal tier is an ethylene–auxin–jasmonate–abscisic acid axis that promotes senescence and is antagonized by cytokinins, brassinosteroids, melatonin, hydrogen sulfide, and the peptide signal CLE14. Its transcriptional tier is dominated by NAC, WRKY, MYB, bHLH, ERF, and TCP factors, exemplified by the BoNAC52–BoRBOHF module that couples hormone signals to reactive oxygen species (ROS) homeostasis. A third tier is post-transcriptional: miRNAs modulate transcript abundance to fine-tune peroxidase activity, chlorophyll biosynthesis, and ROS balance. Third, we connect mechanism to application. We map how preservation technologies (1-MCP, controlled atmospheres, UV and LED irradiation, melatonin, and CLE14) converge on defined molecular nodes. We then ask how the new genomic resources of B. oleracea — the chromosome-scale broccoli reference genome and the 27-genome pan-genome — and CRISPR/Cas9 editing, now demonstrated in broccoli, provide a realistic route toward stay-green, slow-senescing cultivars. We close by identifying six priority frontiers: cell-type-resolved atlases, epigenomic time courses, standardized multi-omics protocols, GWAS for shelf life, rationally designed editing targets, and network-level validation. Broccoli thus illustrates a general arc of postharvest biology, moving from physiology to networks and from networks to designed genotypes.
SN - 1664-462X
ER -