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Peer-reviewedCC BYTranslational vision science & technology

Influence of Room Illumination on Corneal Densitometry.

  • Miriam Esteban-Burgués1
  • Ana R Arizcuren1
  • Cristina Pelayo1
  • Alejandra Consejo1
  1. 1Aragon Institute for Engineering Research, Universidad de Zaragoza, Zaragoza, Spain.
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Abstract

Purpose

To evaluate the effect of room illumination on corneal densitometry measurements obtained with Scheimpflug imaging and optical coherence tomography (OCT) and on corneal geometric parameters.Methods

Twenty-five healthy participants (50 eyes) were imaged under four controlled illumination conditions: extreme scotopic (0.003 lx), dim-light/mesopic (30 lx), low photopic (130 lx), and moderate photopic (220 lx) conditions. Corneal densitometry was quantified as the mean pixel intensity for both the Scheimpflug and the OCT images, with speckle contrast additionally computed for the OCT images. Corneal geometry (central corneal thickness and keratometry readings defined as corneal power in the flattest [K1] and steepest [K2] meridians) was extracted from Scheimpflug tomography. Linear mixed-effects models were used to assess the effect of illumination on corneal parameters.Results

Scheimpflug-derived corneal densitometry increased significantly with illumination (P < 0.001), rising from 45.8 ± 4.3 arbitrary units under extreme scotopic conditions to 69.5 ± 11.5 arbitrary units under moderate photopic illumination. Significant differences were observed between all illumination levels (all P < 0.01). In contrast, the OCT-derived mean pixel intensity and speckle contrast remained stable across conditions (P > 0.05). The corneal geometric parameters showed no significant changes with room illumination (all P > 0.05).Conclusions

Scheimpflug-based corneal densitometry is highly sensitive to room illumination, even within clinically plausible lighting conditions, whereas the OCT-derived metrics are robust to such variations.Translational relevance

Room illumination-induced changes may bias corneal densitometry readings and should be carefully controlled and reported in densitometry studies.

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Cite

BibTeXRIS
BibTeX
@article{estebanburgues2026influence,
  title = {{Influence of Room Illumination on Corneal Densitometry.}},
  author = {Miriam Esteban-Burgués and Ana R Arizcuren and Cristina Pelayo and Alejandra Consejo},
  journal = {Translational vision science \& technology},
  year = {2026},
  month = oct,
  doi = {10.1167/tvst.15.10.2},
  url = {https://europepmc.org/article/MED/42831733},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Influence of Room Illumination on Corneal Densitometry.
AU  - Miriam Esteban-Burgués
AU  - Ana R Arizcuren
AU  - Cristina Pelayo
AU  - Alejandra Consejo
PY  - 2026
DA  - 2026/10/01
JO  - Translational vision science & technology
DO  - 10.1167/tvst.15.10.2
UR  - https://europepmc.org/article/MED/42831733
AB  - Purpose  To evaluate the effect of room illumination on corneal densitometry measurements obtained with Scheimpflug imaging and optical coherence tomography (OCT) and on corneal geometric parameters.Methods  Twenty-five healthy participants (50 eyes) were imaged under four controlled illumination conditions: extreme scotopic (0.003 lx), dim-light/mesopic (30 lx), low photopic (130 lx), and moderate photopic (220 lx) conditions. Corneal densitometry was quantified as the mean pixel intensity for both the Scheimpflug and the OCT images, with speckle contrast additionally computed for the OCT images. Corneal geometry (central corneal thickness and keratometry readings defined as corneal power in the flattest [K1] and steepest [K2] meridians) was extracted from Scheimpflug tomography. Linear mixed-effects models were used to assess the effect of illumination on corneal parameters.Results  Scheimpflug-derived corneal densitometry increased significantly with illumination (P < 0.001), rising from 45.8 ± 4.3 arbitrary units under extreme scotopic conditions to 69.5 ± 11.5 arbitrary units under moderate photopic illumination. Significant differences were observed between all illumination levels (all P < 0.01). In contrast, the OCT-derived mean pixel intensity and speckle contrast remained stable across conditions (P > 0.05). The corneal geometric parameters showed no significant changes with room illumination (all P > 0.05).Conclusions  Scheimpflug-based corneal densitometry is highly sensitive to room illumination, even within clinically plausible lighting conditions, whereas the OCT-derived metrics are robust to such variations.Translational relevance  Room illumination-induced changes may bias corneal densitometry readings and should be carefully controlled and reported in densitometry studies.
SN  - 2164-2591
ER  -