Human tissue-resident CD8 T cells contribute to trophoblast homeostasis in health and during acute inflammation
Abstract
Previous studies have highlighted that some T cell subsets in tissues can provide signals to support tissue cell homeostasis and differentiation. If and how T cell-tissue cell signaling is altered in healthy compared to inflamed tissues is poorly understood. Here, we address if communication between human T cells and tissue cells changes from steady state to an acutely inflamed state in the human placenta. We used single cell analysis strategies to examine invasive cytotrophoblasts (iCTBs) and immune cells isolated from third trimester healthy and acutely inflamed human placentas. We performed cell communication analysis to predict cell-cell communication networks, and found evidence that iCTBs provided signals to support the recruitment of T cells, as well as the formation of tissue-resident memory CD8 T cells (Trm). In exchange, Trm provide signals to support iCTB homeostasis. During acute inflammation, iCTBs and macrophages underwent profound transcriptional changes, while most T cell subsets only underwent limited transcriptional changes. This was not due to T cell exhaustion or tolerance, as T cells were functionally intact. Cell communication analysis and validation at the protein level provide evidence that T cells can maintain their homeostatic support to iCTBs during acute inflammation.
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BibTeX
@misc{dejong2026human,
title = {{Human tissue-resident CD8 T cells contribute to trophoblast homeostasis in health and during acute inflammation}},
author = {Caitlin Dejong and Marie Frutoso and Nicole Potchen and Geervani Daggupati and Andrew Konecny and Yin Huang and Manu Setty and Swati Shree and Stephen Mccartney and Martin Prlic},
howpublished = {bioRxiv : the preprint server for biology},
year = {2026},
month = jun,
doi = {10.64898/2026.06.05.730192},
url = {https://hal.science/hal-05837553v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - Human tissue-resident CD8 T cells contribute to trophoblast homeostasis in health and during acute inflammation
AU - Caitlin Dejong
AU - Marie Frutoso
AU - Nicole Potchen
AU - Geervani Daggupati
AU - Andrew Konecny
AU - Yin Huang
AU - Manu Setty
AU - Swati Shree
AU - Stephen Mccartney
AU - Martin Prlic
PY - 2026
DA - 2026/06/09
PB - bioRxiv : the preprint server for biology
DO - 10.64898/2026.06.05.730192
UR - https://hal.science/hal-05837553v1
AB - Previous studies have highlighted that some T cell subsets in tissues can provide signals to support tissue cell homeostasis and differentiation. If and how T cell-tissue cell signaling is altered in healthy compared to inflamed tissues is poorly understood. Here, we address if communication between human T cells and tissue cells changes from steady state to an acutely inflamed state in the human placenta. We used single cell analysis strategies to examine invasive cytotrophoblasts (iCTBs) and immune cells isolated from third trimester healthy and acutely inflamed human placentas. We performed cell communication analysis to predict cell-cell communication networks, and found evidence that iCTBs provided signals to support the recruitment of T cells, as well as the formation of tissue-resident memory CD8 T cells (Trm). In exchange, Trm provide signals to support iCTB homeostasis. During acute inflammation, iCTBs and macrophages underwent profound transcriptional changes, while most T cell subsets only underwent limited transcriptional changes. This was not due to T cell exhaustion or tolerance, as T cells were functionally intact. Cell communication analysis and validation at the protein level provide evidence that T cells can maintain their homeostatic support to iCTBs during acute inflammation.
SN - 2692-8205
ER -