Ultrasound-assisted deep eutectic solvent extraction of Moringa oleifera leaf polysaccharides: process optimization, purification, physicochemical characterization, and bioactivities.
- 1College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, Heilongjiang, China; Engineering Research Center of Plant-Based Food Processing Education, Qiqihar 161006, Heilongjiang, China. Electronic address: [email protected].
- 2College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, Heilongjiang, China; Engineering Research Center of Plant-Based Food Processing Education, Qiqihar 161006, Heilongjiang, China.
Abstract
Ultrasound-assisted deep eutectic solvent (DES) extraction was developed for the efficient recovery of polysaccharides from Moringa oleifera leaves. Among seven DES systems, choline chloride-urea exhibited the highest extraction efficiency. Response surface methodology further optimized the extraction conditions, achieving a maximum polysaccharide yield of 17.1 %. The crude polysaccharide fraction (MOLP) was subsequently purified by DEAE-52 ion-exchange chromatography and Sephadex G-100 gel filtration, yielding two major purified fractions, MOLPs0 and MOLPs0.5. Structural characterization showed that MOLPs0 was a glucose-dominated neutral polysaccharide fraction, whereas MOLPs0.5 was an acidic heteropolysaccharide enriched in rhamnose and uronic acids. GPC, monosaccharide composition, FT-IR, SEM, AFM, and XRD analyses revealed clear differences in molecular weight distribution, charge properties, surface morphology, and supramolecular organization among the fractions. Bioactivity assays further demonstrated structure-dependent functional differences. Crude MOLP exhibited stronger antioxidant activity and α-glucosidase inhibitory activity, which may be associated with its structural heterogeneity and compositional complexity. In contrast, MOLPs0.5 showed relatively stronger pancreatic lipase inhibitory activity, possibly related to its acidic characteristics and higher uronic acid content. These results suggest that ultrasound-assisted DES extraction combined with chromatographic purification provides an effective strategy for obtaining structurally distinct Moringa oleifera leaf polysaccharides with different functional properties, supporting their potential application as functional food or nutraceutical ingredients.
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BibTeX
@article{liu2026ultrasoundassisted,
title = {{Ultrasound-assisted deep eutectic solvent extraction of Moringa oleifera leaf polysaccharides: process optimization, purification, physicochemical characterization, and bioactivities.}},
author = {Ji Liu and Hongyan Zhang and Yujie Zhi and Wei Wang and Ruwei Zhang and Jian Ren and Yan Wang},
journal = {Ultrasonics sonochemistry},
year = {2026},
month = oct,
doi = {10.1016/j.ultsonch.2026.108100},
url = {https://europepmc.org/article/MED/42874365},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Ultrasound-assisted deep eutectic solvent extraction of Moringa oleifera leaf polysaccharides: process optimization, purification, physicochemical characterization, and bioactivities.
AU - Ji Liu
AU - Hongyan Zhang
AU - Yujie Zhi
AU - Wei Wang
AU - Ruwei Zhang
AU - Jian Ren
AU - Yan Wang
PY - 2026
DA - 2026/10/10
JO - Ultrasonics sonochemistry
DO - 10.1016/j.ultsonch.2026.108100
UR - https://europepmc.org/article/MED/42874365
AB - Ultrasound-assisted deep eutectic solvent (DES) extraction was developed for the efficient recovery of polysaccharides from Moringa oleifera leaves. Among seven DES systems, choline chloride-urea exhibited the highest extraction efficiency. Response surface methodology further optimized the extraction conditions, achieving a maximum polysaccharide yield of 17.1 %. The crude polysaccharide fraction (MOLP) was subsequently purified by DEAE-52 ion-exchange chromatography and Sephadex G-100 gel filtration, yielding two major purified fractions, MOLPs0 and MOLPs0.5. Structural characterization showed that MOLPs0 was a glucose-dominated neutral polysaccharide fraction, whereas MOLPs0.5 was an acidic heteropolysaccharide enriched in rhamnose and uronic acids. GPC, monosaccharide composition, FT-IR, SEM, AFM, and XRD analyses revealed clear differences in molecular weight distribution, charge properties, surface morphology, and supramolecular organization among the fractions. Bioactivity assays further demonstrated structure-dependent functional differences. Crude MOLP exhibited stronger antioxidant activity and α-glucosidase inhibitory activity, which may be associated with its structural heterogeneity and compositional complexity. In contrast, MOLPs0.5 showed relatively stronger pancreatic lipase inhibitory activity, possibly related to its acidic characteristics and higher uronic acid content. These results suggest that ultrasound-assisted DES extraction combined with chromatographic purification provides an effective strategy for obtaining structurally distinct Moringa oleifera leaf polysaccharides with different functional properties, supporting their potential application as functional food or nutraceutical ingredients.
SN - 1350-4177
SN - 1873-2828
ER -