Molecular Architecture of the H2AX-Containing Nucleosome from Arabidopsis.
- 1Department of Human Biosciences, Memorial University of Newfoundland, St John's, NL A1C 5S7, Canada.
- 2Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
- 3Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
Abstract
Nucleosomes integrate diverse histone variants to organize chromatin and coordinate genome maintenance. In plants, the structural interplay between replication-associated histones and DNA damage-responsive histone variants remains poorly understood. Here, we determine cryogenic electron microscopy structures of an Arabidopsis thaliana (At) nucleosome containing the replication-associated histones H2B.6 and H3.1 together with the DNA damage-responsive variant H2AXa. The mononucleosome structure, resolved at 2.73 Å, preserves the canonical nucleosome architecture. Unexpectedly, the reconstituted mononucleosomes formed defined higher-order assemblies in the absence of linker DNA or chemical crosslinking. Two distinct nucleosome-nucleosome arrangements, parallel and right offset, were identified. The right offset closely resembles the human counterpart, whereas the parallel arrangement exhibits slight displacement of H2B structural elements at the inter-nucleosomal interface. Together these structures reveal how a distinct histone composition preserves the canonical nucleosome architecture while supporting multiple defined modes of nucleosome packing, providing structural insights into conserved and divergent features of H2AX-containing chromatin organization across plants and animals.
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BibTeX
@article{panigrahi2026molecular,
title = {{Molecular Architecture of the H2AX-Containing Nucleosome from Arabidopsis.}},
author = {Rashmi Panigrahi and Ross Edwards and Nathaniel Agbagba and Mark Glover},
journal = {Cells},
year = {2026},
month = oct,
doi = {10.3390/cells15191795},
url = {https://europepmc.org/article/MED/42882494},
note = {CC BY},
}
RIS
TY - JOUR
TI - Molecular Architecture of the H2AX-Containing Nucleosome from Arabidopsis.
AU - Rashmi Panigrahi
AU - Ross Edwards
AU - Nathaniel Agbagba
AU - Mark Glover
PY - 2026
DA - 2026/10/01
JO - Cells
DO - 10.3390/cells15191795
UR - https://europepmc.org/article/MED/42882494
AB - Nucleosomes integrate diverse histone variants to organize chromatin and coordinate genome maintenance. In plants, the structural interplay between replication-associated histones and DNA damage-responsive histone variants remains poorly understood. Here, we determine cryogenic electron microscopy structures of an Arabidopsis thaliana (At) nucleosome containing the replication-associated histones H2B.6 and H3.1 together with the DNA damage-responsive variant H2AXa. The mononucleosome structure, resolved at 2.73 Å, preserves the canonical nucleosome architecture. Unexpectedly, the reconstituted mononucleosomes formed defined higher-order assemblies in the absence of linker DNA or chemical crosslinking. Two distinct nucleosome-nucleosome arrangements, parallel and right offset, were identified. The right offset closely resembles the human counterpart, whereas the parallel arrangement exhibits slight displacement of H2B structural elements at the inter-nucleosomal interface. Together these structures reveal how a distinct histone composition preserves the canonical nucleosome architecture while supporting multiple defined modes of nucleosome packing, providing structural insights into conserved and divergent features of H2AX-containing chromatin organization across plants and animals.
SN - 2073-4409
ER -