Identification of a Novel Placental Peptide with Skin Reparative Properties by Cell-Surface Affinity Screening.
- 1State Key Laboratory of Biopharmaceutical Preparation and Delivery, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
- 2School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract
Placental extracts are rich in bioactive molecules and exhibit broad reparative properties, yet the molecular constituents responsible for their effects on skin tissue repair remain insufficiently characterized. In this study, we employed a cell-surface affinity-based screening approach using dermal fibroblasts (BJ) and microvascular endothelial (HMEC-1) cells as dual biological baits to capture bioactive peptides directly from placental extracts. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and background subtraction, a peptide with dual-cell targeting potential, GPPVSE, was identified. In vitro, GPPVSE significantly promoted the viability, proliferation and migration of both BJ and HMEC-1 cells and enhanced endothelial tube formation. In vivo, topical 20 μM GPPVSE in a mouse full-thickness skin defect model achieved an 84.7% wound closure rate by day 8, outperforming both phosphate-buffered saline (PBS) and epidermal growth factor (EGF) groups. Histological analysis revealed that GPPVSE promoted granulation tissue maturation, strengthened epidermal-dermal junction integrity, and enhanced regeneration of skin appendages, including hair follicles and sebaceous glands, during the remodeling phase. Collectively, GPPVSE provides valuable molecular insights into the reparative activity of placental extracts and establishes a potentially adaptable cell-based paradigm for mining functional peptides from complex natural sources.
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BibTeX
@article{xing2026identification,
title = {{Identification of a Novel Placental Peptide with Skin Reparative Properties by Cell-Surface Affinity Screening.}},
author = {Fangyu Xing and Jianping Gao and Zhenhu Guo and Yingjun Kong and Guifeng Zhang},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198617},
url = {https://europepmc.org/article/MED/42883864},
note = {CC BY},
}
RIS
TY - JOUR
TI - Identification of a Novel Placental Peptide with Skin Reparative Properties by Cell-Surface Affinity Screening.
AU - Fangyu Xing
AU - Jianping Gao
AU - Zhenhu Guo
AU - Yingjun Kong
AU - Guifeng Zhang
PY - 2026
DA - 2026/09/26
JO - International journal of molecular sciences
DO - 10.3390/ijms27198617
UR - https://europepmc.org/article/MED/42883864
AB - Placental extracts are rich in bioactive molecules and exhibit broad reparative properties, yet the molecular constituents responsible for their effects on skin tissue repair remain insufficiently characterized. In this study, we employed a cell-surface affinity-based screening approach using dermal fibroblasts (BJ) and microvascular endothelial (HMEC-1) cells as dual biological baits to capture bioactive peptides directly from placental extracts. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and background subtraction, a peptide with dual-cell targeting potential, GPPVSE, was identified. In vitro, GPPVSE significantly promoted the viability, proliferation and migration of both BJ and HMEC-1 cells and enhanced endothelial tube formation. In vivo, topical 20 μM GPPVSE in a mouse full-thickness skin defect model achieved an 84.7% wound closure rate by day 8, outperforming both phosphate-buffered saline (PBS) and epidermal growth factor (EGF) groups. Histological analysis revealed that GPPVSE promoted granulation tissue maturation, strengthened epidermal-dermal junction integrity, and enhanced regeneration of skin appendages, including hair follicles and sebaceous glands, during the remodeling phase. Collectively, GPPVSE provides valuable molecular insights into the reparative activity of placental extracts and establishes a potentially adaptable cell-based paradigm for mining functional peptides from complex natural sources.
SN - 1422-0067
ER -