Draft genome sequences and pan-genome of Bacillus Bac5 and Bac6 strains isolated from a mangrove ecosystem with anti-Vibrio activity
- 1Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador
- 2Facultad de Ciencias Agropecuarias, Universidad Técnica de Machala, Machala, Ecuador
Abstract
Two Bacillus strains (Bacillus velezensis Bac5 and Bacillus subtilis Bac6) displaying antagonistic activity against Vibrio spp. were isolated from mangrove sediments in Ecuador. Here, we report the draft genome sequences and provide a comprehensive genomic characterization. The genome of strain Bac5 has a size of 3889,715 bp assembled into 15 contigs, while Bac6 has a genome size of 4384,991 bp assembled into 20 contigs. Genome annotation identified 4 and 5 virulence-associated genes, respectively, including genes related to motility, iron acquisition, capsule formation, stress response, and toxin-associated regulators commonly reported in Bacillus species. In addition, several antimicrobial resistance-related genes were detected. The most abundant COG functional categories in both strains were transcription, amino acid transport and metabolism, and carbohydrate transport and metabolism, indicating metabolic versatility. To further contextualize these findings, a comparative pan-genome analysis was performed, including our genomes and publicly available B. velezensis and B. subtilis strains associated with aquaculture and other applications. However, gene association analysis did not identify statistically significant markers linked to aquaculture-associated strains. Biosynthetic gene cluster (BGC) analysis revealed that both Bac5 and Bac6 harbor a conserved repertoire of antimicrobial clusters commonly reported in the species, including lipopeptides and polyketides, consistent with their antagonistic activity. In contrast, genomic differences were observed between B. velezensis and B. subtilis, reflecting species-level divergence rather than traits associated with probiotic function. The objective of this study was to sequence and characterize the genomes of two B. velezensis (Bac5) and B. subtilis (Bac6) strains with anti-Vibrio activity, and to compare them with genomes from aquaculture-associated and other environments through pangenome and biosynthetic gene cluster analyzes. This dataset enables comparative genomic analyzes of probiotic-associated features, highlighting a conserved core genome and a variable accessory gene repertoire across strains from different ecological and applied contexts.
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BibTeX
@article{reyes2026draft,
title = {{Draft genome sequences and pan-genome of Bacillus Bac5 and Bac6 strains isolated from a mangrove ecosystem with anti-Vibrio activity}},
author = {Guillermo Reyes and Lita Sorroza},
journal = {Data in Brief},
year = {2026},
month = oct,
doi = {10.1016/j.dib.2026.113363},
url = {https://doi.org/10.1016/j.dib.2026.113363},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Draft genome sequences and pan-genome of Bacillus Bac5 and Bac6 strains isolated from a mangrove ecosystem with anti-Vibrio activity
AU - Guillermo Reyes
AU - Lita Sorroza
PY - 2026
DA - 2026/10/22
JO - Data in Brief
DO - 10.1016/j.dib.2026.113363
UR - https://doi.org/10.1016/j.dib.2026.113363
AB - Two Bacillus strains (Bacillus velezensis Bac5 and Bacillus subtilis Bac6) displaying antagonistic activity against Vibrio spp. were isolated from mangrove sediments in Ecuador. Here, we report the draft genome sequences and provide a comprehensive genomic characterization. The genome of strain Bac5 has a size of 3889,715 bp assembled into 15 contigs, while Bac6 has a genome size of 4384,991 bp assembled into 20 contigs. Genome annotation identified 4 and 5 virulence-associated genes, respectively, including genes related to motility, iron acquisition, capsule formation, stress response, and toxin-associated regulators commonly reported in Bacillus species. In addition, several antimicrobial resistance-related genes were detected. The most abundant COG functional categories in both strains were transcription, amino acid transport and metabolism, and carbohydrate transport and metabolism, indicating metabolic versatility. To further contextualize these findings, a comparative pan-genome analysis was performed, including our genomes and publicly available B. velezensis and B. subtilis strains associated with aquaculture and other applications. However, gene association analysis did not identify statistically significant markers linked to aquaculture-associated strains. Biosynthetic gene cluster (BGC) analysis revealed that both Bac5 and Bac6 harbor a conserved repertoire of antimicrobial clusters commonly reported in the species, including lipopeptides and polyketides, consistent with their antagonistic activity. In contrast, genomic differences were observed between B. velezensis and B. subtilis, reflecting species-level divergence rather than traits associated with probiotic function. The objective of this study was to sequence and characterize the genomes of two B. velezensis (Bac5) and B. subtilis (Bac6) strains with anti-Vibrio activity, and to compare them with genomes from aquaculture-associated and other environments through pangenome and biosynthetic gene cluster analyzes. This dataset enables comparative genomic analyzes of probiotic-associated features, highlighting a conserved core genome and a variable accessory gene repertoire across strains from different ecological and applied contexts.
SN - 2352-3409
ER -