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Peer-reviewedCC BYFrontiers in allergy

A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT.

  • Tetsuro Yamamoto1,2
  • Fusako Mitsunaga3,4
  • Atsushi Kotani1,2
  • Kazuki Tajima1,2
  • Muneki Hotomi5
  • Shin Nakamura3,4
5 affiliations
  1. 1Research Center, EPS Holdings, Inc., Bunkyo-ku, Tokyo, Japan.
  2. 2Research Center, EPS Innovative Medicine (Japan) Co., Ltd., Shinjuku-ku, Tokyo, Japan.
  3. 3Intelligence and Technology Lab Inc., Kaizu, Gifu, Japan.
  4. 4Biomedical Institute, NPO Primate Agora, Kaizu, Gifu, Japan.
  5. 5Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
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Abstract

Background

Sublingual immunotherapy (SLIT) is safe and one of the most effective etiological treatment for pollinosis. Allergen-specific salivary secretory immunoglobulin A (SIgA) is a useful marker to objectively evaluate treatment efficacy. However, saliva samples usually exhibit high background noise because of non-specific binding in enzyme-linked immunosorbent assays (ELISAs). We therefore aimed to establish an amplified ELISA to measure allergen-specific salivary SIgA levels to assess the efficacy of SLIT for Japanese cedar pollinosis.Methods

Biotinyl-tyramide (BT-Tyr) was used to amplify the signal in a colorimetric ELISA. The allergen of Japanese cedar pollen, Sugi basic protein (SBP), was biotinylated and used as a reporter to obtain sensitive and specific results with low background. To obtain preliminary observations, we applied this ELISA for measuring anti-SBP SIgA levels in saliva samples from SLIT-treated patients with Japanese cedar pollinosis.Results

The ELISA was highly sensitive and achieved more than 200-fold signal amplification compared with non-amplified conditions. It was also specific for antigens without background noise. The levels of SBP-specific SIgA in saliva increased in patients 9 months after SLIT initiation at the latest.Conclusions

We developed a sensitive colorimetric ELISA to measure SBP-specific SIgA in saliva using BT-Tyr-based amplification and biotinylated antigen as a reporter. We successfully applied this sensitive ELISA to monitor the levels of SBP-specific SIgA in saliva samples from patients with pollinosis receiving SLIT. Increased salivary SBP-specific SIgA levels were observed in these patients.

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Cite

BibTeXRIS
BibTeX
@article{yamamoto2026sensitive,
  title = {{A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT.}},
  author = {Tetsuro Yamamoto and Fusako Mitsunaga and Atsushi Kotani and Kazuki Tajima and Muneki Hotomi and Shin Nakamura},
  journal = {Frontiers in allergy},
  year = {2026},
  month = oct,
  doi = {10.3389/falgy.2026.1857343},
  url = {https://europepmc.org/article/MED/42898977},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT.
AU  - Tetsuro Yamamoto
AU  - Fusako Mitsunaga
AU  - Atsushi Kotani
AU  - Kazuki Tajima
AU  - Muneki Hotomi
AU  - Shin Nakamura
PY  - 2026
DA  - 2026/10/05
JO  - Frontiers in allergy
DO  - 10.3389/falgy.2026.1857343
UR  - https://europepmc.org/article/MED/42898977
AB  - Background  Sublingual immunotherapy (SLIT) is safe and one of the most effective etiological treatment for pollinosis. Allergen-specific salivary secretory immunoglobulin A (SIgA) is a useful marker to objectively evaluate treatment efficacy. However, saliva samples usually exhibit high background noise because of non-specific binding in enzyme-linked immunosorbent assays (ELISAs). We therefore aimed to establish an amplified ELISA to measure allergen-specific salivary SIgA levels to assess the efficacy of SLIT for Japanese cedar pollinosis.Methods  Biotinyl-tyramide (BT-Tyr) was used to amplify the signal in a colorimetric ELISA. The allergen of Japanese cedar pollen, Sugi basic protein (SBP), was biotinylated and used as a reporter to obtain sensitive and specific results with low background. To obtain preliminary observations, we applied this ELISA for measuring anti-SBP SIgA levels in saliva samples from SLIT-treated patients with Japanese cedar pollinosis.Results  The ELISA was highly sensitive and achieved more than 200-fold signal amplification compared with non-amplified conditions. It was also specific for antigens without background noise. The levels of SBP-specific SIgA in saliva increased in patients 9 months after SLIT initiation at the latest.Conclusions  We developed a sensitive colorimetric ELISA to measure SBP-specific SIgA in saliva using BT-Tyr-based amplification and biotinylated antigen as a reporter. We successfully applied this sensitive ELISA to monitor the levels of SBP-specific SIgA in saliva samples from patients with pollinosis receiving SLIT. Increased salivary SBP-specific SIgA levels were observed in these patients.
SN  - 2673-6101
ER  -