In situ multiscale XCT degradation study of PEO-modified WE43 scaffolds: Correlating microstructure with hydrogen evolution
Abstract
The degradation of magnesium-based implants for bone regeneration leads to hydrogen gas evolution, prompting local pH shifts, oxidative stress, and reduced mechanical integrity, factors that collectively impair cell function and tissue healing. While numerous studies aim to mitigate hydrogen release, few provide three-dimensional (3D), real-time visualization of hydrogen bubble formation in direct relation to scaffold microstructure. In this study, we present a multiscale in situ imaging approach to address this gap. Timeresolved tomography was employed to monitor hydrogen bubble nucleation and growth during magnesium scaffold degradation in simulated body fluid (SBF). Simultaneously, X-ray nano-tomography revealed scaffold microstructural features, such as intermetallic precipitates, porosity, and grain oxide inclusions, at high spatial resolution. These features not only influence degradation behavior but also play a role in the formation and uniformity of the plasma electrolytic oxidation (PEO) layer, which serves as a protective barrier. By integrating both datasets, we correlated specific microstructural characteristics with hydrogen bubble dynamics and local degradation performance. Our findings identify the degradation initiation sites and the underlying corrosion mechanisms within a 3D scaffold architecture. This combined methodology provides unprecedented insights into the interplay between microstructure, degradation and PEO layer, supporting the development of more reliable, long-lasting magnesium-based implants. Unlike previous studies that have focused on post-mortem surface characterization or overall corrosion rate measurements, this work enables in situ, time-resolved 3D observation of hydrogen evolution within additively manufactured Mg scaffolds, establishing direct correlations between LPBF-induced microstructure, PEO layer uniformity, and local degradation mechanisms.
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BibTeX
@article{martinalonso2026situ,
title = {{In situ multiscale XCT degradation study of PEO-modified WE43 scaffolds: Correlating microstructure with hydrogen evolution}},
author = {M.D. Martin-Alonso and G. Dominguez and F. Benn and S. Pöstges and A. Kopp and J. Villanova and Pierre Lhuissier and J. Molina-Aldareguia and F. Sket},
journal = {Additive Manufacturing},
year = {2026},
month = sep,
doi = {10.1016/j.addma.2025.105042},
url = {https://hal.science/hal-05760606v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - In situ multiscale XCT degradation study of PEO-modified WE43 scaffolds: Correlating microstructure with hydrogen evolution
AU - M.D. Martin-Alonso
AU - G. Dominguez
AU - F. Benn
AU - S. Pöstges
AU - A. Kopp
AU - J. Villanova
AU - Pierre Lhuissier
AU - J. Molina-Aldareguia
AU - F. Sket
PY - 2026
DA - 2026/09/23
JO - Additive Manufacturing
DO - 10.1016/j.addma.2025.105042
UR - https://hal.science/hal-05760606v1
AB - The degradation of magnesium-based implants for bone regeneration leads to hydrogen gas evolution, prompting local pH shifts, oxidative stress, and reduced mechanical integrity, factors that collectively impair cell function and tissue healing. While numerous studies aim to mitigate hydrogen release, few provide three-dimensional (3D), real-time visualization of hydrogen bubble formation in direct relation to scaffold microstructure. In this study, we present a multiscale in situ imaging approach to address this gap. Timeresolved tomography was employed to monitor hydrogen bubble nucleation and growth during magnesium scaffold degradation in simulated body fluid (SBF). Simultaneously, X-ray nano-tomography revealed scaffold microstructural features, such as intermetallic precipitates, porosity, and grain oxide inclusions, at high spatial resolution. These features not only influence degradation behavior but also play a role in the formation and uniformity of the plasma electrolytic oxidation (PEO) layer, which serves as a protective barrier. By integrating both datasets, we correlated specific microstructural characteristics with hydrogen bubble dynamics and local degradation performance. Our findings identify the degradation initiation sites and the underlying corrosion mechanisms within a 3D scaffold architecture. This combined methodology provides unprecedented insights into the interplay between microstructure, degradation and PEO layer, supporting the development of more reliable, long-lasting magnesium-based implants. Unlike previous studies that have focused on post-mortem surface characterization or overall corrosion rate measurements, this work enables in situ, time-resolved 3D observation of hydrogen evolution within additively manufactured Mg scaffolds, establishing direct correlations between LPBF-induced microstructure, PEO layer uniformity, and local degradation mechanisms.
SN - 2214-8604
SN - 2214-7810
ER -