Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 3 min ago
Peer-reviewedCC BY 4.0Journal of Clinical Medicine

Differences in Pulp Wound Healing and Regenerative Potential Between SHEDs and DPSCs

  • Rosa Baldeon-Gutierrez1
  • Masahiko Terajima2
  • Yuria Hori1
  • Ryo Bando1
  • Yuki Kiyokawa1
  • Emi Inada3
  • Yoko Iwase4
  • Tomoya Murakami5
  • Satoshi Watanabe6
  • Naoko Kubota3
  • Hirofumi Noguchi7
  • Masahiro Sato8
1 more authors
  • Issei Saitoh1
8 affiliations
  1. 1Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan
  2. 2Department of Anatomy, Asahi University School of Dentistry, Gifu 501-0296, Japan
  3. 3Department of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan
  4. 4Department of Dentistry for the Disability and Oral Health, Asahi University School of Dentistry, Gifu 501-0296, Japan
  5. 5Kyoto Dental Service Center Central Clinic, Kyoto 604-8418, Japan
  6. 6Institute of Livestock and Grassland Science, Tsukuba 305-0901, Japan
  7. 7Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan
  8. 8Department of Genome Medicine, National Center for Child Health and Development, Tokyo 157-8535, Japan
Read full textPDFDOICite

Abstract

Several challenges limit effective pulp wound healing during vital pulp therapies (VPTs) and pulp regeneration in necrotic teeth. Recent advances in regenerative therapies using dental mesenchymal stem cells (MSCs) have shown promise for improving tissue repair and expanding minimally invasive treatment strategies. Among these cells, stem cells from human exfoliated deciduous teeth (SHEDs) and dental pulp stem cells (DPSCs) have gained attention for cell-based VPTs (CB-VPTs) and cell-based regenerative endodontic procedures (CB-REPs). However, little is known about whether SHEDs or DPSCs perform better in CB-VPTs and CB-REPs, particularly regarding wound healing and dentin–pulp regeneration. To date, few studies have compared SHED and DPSC outcomes; separately, CB-VPTs and CB-REPs have shown promising outcomes with both cell types. Dentin bridge formation was generally observed following cell-free approaches, whereas recent in vivo studies of CB-VPTs and CB-REPs have additionally demonstrated the formation of pulp-like tissue. Histological analysis in animal models has identified odontoblasts, angiogenesis, neurogenesis, and connective tissue following CB-REPs using SHEDs or DPSCs. In humans, histological evidence of pulp-like tissue regeneration after SHED CB-REPs has been reported in a single patient. Collectively, preclinical findings and limited clinical evidence suggested that CB-REPs may support injury resolution accompanied by histological regeneration of pulp-like tissue. Both SHEDs and DPSCs represent promising candidates for CB-VPTs and CB-REPs; however, further comparative studies and randomized clinical trials are needed to strengthen the evidence on clinical effectiveness. This review examines the biological properties and regenerative potential of SHEDs and DPSCs within the dentin–pulp complex.

Related

  1. A pro-regenerative petroleum jelly-based, copper-doped bioactive glass ointment for impaired wound healing in metabolic syndrome
    Huifeng Wang, Ophelia Tong, Youssef Ibrahim +11
    Materials Today BioPeer-reviewedCC BY-NC-NDFull textGeneral medicineDOAJEurope PMCPMC
  2. Plant-derived modulators of the hemostasis-wound healing axis: mechanisms, evidence, and translational potential
    Zoila Mora-Guzmán, Ibzan Jahzeel Salvador Ibarra, Lilian Dolores Chel‐Guerrero +6
    Frontiers in pharmacologyPeer-reviewedCC BYFull textPharmacology & toxicologyEurope PMC
  3. Physicochemical Characterization, Microencapsulation, and In-vitro Wound Healing Potential of Cold-Pressed Industrial Hemp Seed Oil
    Erman Duman, Atilla Evcin, Sabire Duman +5
    Journal of Oleo SciencePeer-reviewedCC BY-SAFull textGeneral medicineJ-STAGE
  4. Development and Characterization of Phlomis brachyodon Extract-Loaded Thermoresponsive In Situ Gels: Antioxidant Activity, Wound-Healing Evaluation in a Rat Excision Model, and Potential Anticancer Activity.
    Nour Aboalhaija, Fatimah Al-Dulaimi, Osama H Abusara +5
    Gels (Basel, Switzerland)Peer-reviewedCC BYFull textGeneral medicineEurope PMC
  5. Synthesis, Characterization, and Multifunctional Applications of Chitosan-Grafted-Poly(Acrylic Acid-Co-Acrylamide)/ZnO Nanocomposite Hydrogel: Loading Studies, Antimicrobial Activity, Wound Healing, and Anticancer Potential
    Asaad H Alzaidy, Safaa H Ganduh, Murtadha Saleh Hussien +4
    International Journal of NanomedicinePeer-reviewedCC BY-NC 4.0Full textGeneral medicinePMCEurope PMC

Cite

BibTeXRIS
BibTeX
@article{baldeongutierrez2026differences,
  title = {{Differences in Pulp Wound Healing and Regenerative Potential Between SHEDs and DPSCs}},
  author = {Rosa Baldeon-Gutierrez and Masahiko Terajima and Yuria Hori and Ryo Bando and Yuki Kiyokawa and Emi Inada and Yoko Iwase and Tomoya Murakami and Satoshi Watanabe and Naoko Kubota and Hirofumi Noguchi and Masahiro Sato and Issei Saitoh},
  journal = {Journal of Clinical Medicine},
  year = {2026},
  month = oct,
  doi = {10.3390/jcm15208162},
  url = {https://www.mdpi.com/2077-0383/15/20/8162},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Differences in Pulp Wound Healing and Regenerative Potential Between SHEDs and DPSCs
AU  - Rosa Baldeon-Gutierrez
AU  - Masahiko Terajima
AU  - Yuria Hori
AU  - Ryo Bando
AU  - Yuki Kiyokawa
AU  - Emi Inada
AU  - Yoko Iwase
AU  - Tomoya Murakami
AU  - Satoshi Watanabe
AU  - Naoko Kubota
AU  - Hirofumi Noguchi
AU  - Masahiro Sato
AU  - Issei Saitoh
PY  - 2026
DA  - 2026/10/01
JO  - Journal of Clinical Medicine
DO  - 10.3390/jcm15208162
UR  - https://www.mdpi.com/2077-0383/15/20/8162
AB  - Several challenges limit effective pulp wound healing during vital pulp therapies (VPTs) and pulp regeneration in necrotic teeth. Recent advances in regenerative therapies using dental mesenchymal stem cells (MSCs) have shown promise for improving tissue repair and expanding minimally invasive treatment strategies. Among these cells, stem cells from human exfoliated deciduous teeth (SHEDs) and dental pulp stem cells (DPSCs) have gained attention for cell-based VPTs (CB-VPTs) and cell-based regenerative endodontic procedures (CB-REPs). However, little is known about whether SHEDs or DPSCs perform better in CB-VPTs and CB-REPs, particularly regarding wound healing and dentin–pulp regeneration. To date, few studies have compared SHED and DPSC outcomes; separately, CB-VPTs and CB-REPs have shown promising outcomes with both cell types. Dentin bridge formation was generally observed following cell-free approaches, whereas recent in vivo studies of CB-VPTs and CB-REPs have additionally demonstrated the formation of pulp-like tissue. Histological analysis in animal models has identified odontoblasts, angiogenesis, neurogenesis, and connective tissue following CB-REPs using SHEDs or DPSCs. In humans, histological evidence of pulp-like tissue regeneration after SHED CB-REPs has been reported in a single patient. Collectively, preclinical findings and limited clinical evidence suggested that CB-REPs may support injury resolution accompanied by histological regeneration of pulp-like tissue. Both SHEDs and DPSCs represent promising candidates for CB-VPTs and CB-REPs; however, further comparative studies and randomized clinical trials are needed to strengthen the evidence on clinical effectiveness. This review examines the biological properties and regenerative potential of SHEDs and DPSCs within the dentin–pulp complex.
SN  - 2077-0383
ER  -