A high-confidence plant mitochondrial proteome via protein correlation profiling
4 affiliations
- 1Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
- 2Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
- 3Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
- 4Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: [email protected]
Abstract
Plant mitochondria share many features with animal mitochondria but also possess unique traits, including presence of both circular and linear mitochondrial DNA, RNA editing, a branched electron transport chain, and metabolic pathways supporting photosynthesis. An accurate mitochondrial proteome is crucial for studying these conserved and lineage-specific functions. Here, we introduce a novel approach to defining plant mitoproteomes that combines serial mitochondrial enrichment, mass spectrometry, and protein correlation profiling. This approach allows us to experimentally define a mitochondrial protein inventory of 1,462 proteins with high sensitivity and specificity. Integration of our experimental inventory with literature and microscopy validation yields the Arabidopsisthaliana MitoCarta (AtMitoCarta) atlas of 1,609 mitochondrial proteins, including 236 newly defined proteins and 147 proteins of unknown functions. A comparative analysis across eukaryotic organisms reveals that 15% of mitochondrial protein families are plant specific, including expanded pentatricopeptide repeat proteins. The AtMitoCarta inventory and the experimental workflow applicable to wild-type plants will enable comparative studies of plant mitochondria.
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BibTeX
@article{kim2026highconfidence,
title = {{A high-confidence plant mitochondrial proteome via protein correlation profiling}},
author = {Minsoo Kim and Sarah E. Calvo and Khanh Nguyen and Keith D. Rivera and Namrata D. Udeshi and Ruslan Aphasizhev and Inna Aphasizheva and Cora N. Betsinger and Steven A. Carr and Luke H. Chao and Michael Z. Chen and Felicia G. Deng and Manoj T. Duraisingh and Michelle Y. Fry and Manish Goyal and Tiana M. Issa and Cameron Genxuan Lian and Yi-Ting Liao and Bridget E Luce and Syed Yusuf Mian and Aditya S. Paul and John Crawford Samuelson and Md Solayman and Jonathan A. Stefely and Andres F. Vacas and Vamsi Krishna Mootha},
journal = {Cell},
year = {2026},
month = oct,
doi = {10.1016/j.cell.2026.08.027},
url = {https://doi.org/10.1016/j.cell.2026.08.027},
note = {CC BY},
}
RIS
TY - JOUR
TI - A high-confidence plant mitochondrial proteome via protein correlation profiling
AU - Minsoo Kim
AU - Sarah E. Calvo
AU - Khanh Nguyen
AU - Keith D. Rivera
AU - Namrata D. Udeshi
AU - Ruslan Aphasizhev
AU - Inna Aphasizheva
AU - Cora N. Betsinger
AU - Steven A. Carr
AU - Luke H. Chao
AU - Michael Z. Chen
AU - Felicia G. Deng
AU - Manoj T. Duraisingh
AU - Michelle Y. Fry
AU - Manish Goyal
AU - Tiana M. Issa
AU - Cameron Genxuan Lian
AU - Yi-Ting Liao
AU - Bridget E Luce
AU - Syed Yusuf Mian
AU - Aditya S. Paul
AU - John Crawford Samuelson
AU - Md Solayman
AU - Jonathan A. Stefely
AU - Andres F. Vacas
AU - Vamsi Krishna Mootha
PY - 2026
DA - 2026/10/01
JO - Cell
DO - 10.1016/j.cell.2026.08.027
UR - https://doi.org/10.1016/j.cell.2026.08.027
AB - Plant mitochondria share many features with animal mitochondria but also possess unique traits, including presence of both circular and linear mitochondrial DNA, RNA editing, a branched electron transport chain, and metabolic pathways supporting photosynthesis. An accurate mitochondrial proteome is crucial for studying these conserved and lineage-specific functions. Here, we introduce a novel approach to defining plant mitoproteomes that combines serial mitochondrial enrichment, mass spectrometry, and protein correlation profiling. This approach allows us to experimentally define a mitochondrial protein inventory of 1,462 proteins with high sensitivity and specificity. Integration of our experimental inventory with literature and microscopy validation yields the Arabidopsisthaliana MitoCarta (AtMitoCarta) atlas of 1,609 mitochondrial proteins, including 236 newly defined proteins and 147 proteins of unknown functions. A comparative analysis across eukaryotic organisms reveals that 15% of mitochondrial protein families are plant specific, including expanded pentatricopeptide repeat proteins. The AtMitoCarta inventory and the experimental workflow applicable to wild-type plants will enable comparative studies of plant mitochondria.
SN - 0092-8674
SN - 1097-4172
ER -