Extract of Cinnamomum cassia L. possesses an inhibitor of pancreatic lipase and α-glucosidase that acts as a potential antidiabesity agent
Abstract
Background: Diabesity, defined as the coexistence of obesity and type 2 diabetes mellitus (T2DM), has emerged as a major global health concern. Pancreatic lipase and α-glucosidase are recognized as key therapeutic targets for obesity and T2DM, respectively. Oxidative stress also plays a significant role in the pathogenesis of these conditions. This study was designed to evaluate the antidiabesity potential of sequential extracts of Cinnamomum cassia (C. cassia) bark by assessing inhibition of pancreatic lipase and α-glucosidase, as well as antioxidant activity. Materials and Methods: Sequential extracts of C. cassia bark were prepared using different solvent systems. Pancreatic lipase activity was measured using the synthetic substrate p-nitrophenyl palmitate, and inhibition was evaluated accordingly. α-Glucosidase inhibition was assessed using p-nitrophenyl α-d-glucopyranoside as the substrate. Antioxidant activity of the selected extract was evaluated using superoxide and free radical-scavenging assays, as described in the methodology. Phytochemical constituents were identified using standard procedures, and the antidiabetes-active component was localized by thin-layer chromatography (TLC). Furthermore, attempts were made to isolate and characterize the active multifunctional compound using Fourier transform infrared (FTIR) spectroscopy and phytochemical profiling. Results: Among all extracts, the methanolic extract showed the highest biological activity at 100 µg/mL. It exhibited 66% inhibition of pancreatic lipase (IC50 = 67.95 µg/mL) with the synthetic substrate and 81.7% with the natural substrate. Complete (100%) α-glucosidase inhibition was observed (IC50 = 12.46 µg/mL). The extract also showed strong antioxidant activity, with 78.2% superoxide-scavenging activity and 87.9% free radical-scavenging activity. Phytochemical analysis confirmed the presence of multiple bioactive compounds. Notably, TLC revealed a single active, multifunctional band at an Rf value of 0.75, identified as a phenolic compound. FTIR analysis further supported the presence of hydroxyl and aldehyde functional groups. Conclusion: These findings suggest that the methanolic extract of C. cassia bark contains a phenolic compound (Rf = 0.75) with significant antidiabesity potential.
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BibTeX
@article{rathod2026extract,
title = {{Extract of Cinnamomum cassia L. possesses an inhibitor of pancreatic lipase and α-glucosidase that acts as a potential antidiabesity agent}},
author = {Priyanka Rathod and Chandana C. Kulkarni and Raman P. Yadav},
journal = {MGM Journal of Medical Sciences},
year = {2026},
month = jul,
doi = {10.4103/mgmj.mgmj_92_26},
url = {https://doi.org/10.4103/mgmj.mgmj_92_26},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Extract of Cinnamomum cassia L. possesses an inhibitor of pancreatic lipase and α-glucosidase that acts as a potential antidiabesity agent
AU - Priyanka Rathod
AU - Chandana C. Kulkarni
AU - Raman P. Yadav
PY - 2026
DA - 2026/07/01
JO - MGM Journal of Medical Sciences
DO - 10.4103/mgmj.mgmj_92_26
UR - https://doi.org/10.4103/mgmj.mgmj_92_26
AB - Background: Diabesity, defined as the coexistence of obesity and type 2 diabetes mellitus (T2DM), has emerged as a major global health concern. Pancreatic lipase and α-glucosidase are recognized as key therapeutic targets for obesity and T2DM, respectively. Oxidative stress also plays a significant role in the pathogenesis of these conditions. This study was designed to evaluate the antidiabesity potential of sequential extracts of Cinnamomum cassia (C. cassia) bark by assessing inhibition of pancreatic lipase and α-glucosidase, as well as antioxidant activity. Materials and Methods: Sequential extracts of C. cassia bark were prepared using different solvent systems. Pancreatic lipase activity was measured using the synthetic substrate p-nitrophenyl palmitate, and inhibition was evaluated accordingly. α-Glucosidase inhibition was assessed using p-nitrophenyl α-d-glucopyranoside as the substrate. Antioxidant activity of the selected extract was evaluated using superoxide and free radical-scavenging assays, as described in the methodology. Phytochemical constituents were identified using standard procedures, and the antidiabetes-active component was localized by thin-layer chromatography (TLC). Furthermore, attempts were made to isolate and characterize the active multifunctional compound using Fourier transform infrared (FTIR) spectroscopy and phytochemical profiling. Results: Among all extracts, the methanolic extract showed the highest biological activity at 100 µg/mL. It exhibited 66% inhibition of pancreatic lipase (IC50 = 67.95 µg/mL) with the synthetic substrate and 81.7% with the natural substrate. Complete (100%) α-glucosidase inhibition was observed (IC50 = 12.46 µg/mL). The extract also showed strong antioxidant activity, with 78.2% superoxide-scavenging activity and 87.9% free radical-scavenging activity. Phytochemical analysis confirmed the presence of multiple bioactive compounds. Notably, TLC revealed a single active, multifunctional band at an Rf value of 0.75, identified as a phenolic compound. FTIR analysis further supported the presence of hydroxyl and aldehyde functional groups. Conclusion: These findings suggest that the methanolic extract of C. cassia bark contains a phenolic compound (Rf = 0.75) with significant antidiabesity potential.
SN - 2347-7946
SN - 2347-7962
ER -