Different Photons, Same Chemistry: Identical Mechanism and Efficiency of Fatty Acid Photodecarboxylase under Blue and UV Excitation
Abstract
Fatty acid photodecarboxylase (FAP) is a promising photoenzyme for light-driven hydrocarbon synthesis and a versatile chassis for photobiocatalysis. A recent study proposed that UV/violet illumination enhances FAP activity relative to blue light by accessing a distinct, more efficient photochemical regime. Here, steady-state and time-resolved fluorescence, transient absorption spectroscopy under single-turnover conditions, and product quantification under continuous irradiation show that the quantum yield of decarboxylation, the identities and kinetics of reaction intermediates, and the overall chemical yield are identical for blue and UV/violet excitation. These findings establish that FAP operates through a single, wavelength-independent photocycle, overturning the proposed "violet-enhanced" reactivity model.
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BibTeX
@article{michielin2026different,
title = {{Different Photons, Same Chemistry: Identical Mechanism and Efficiency of Fatty Acid Photodecarboxylase under Blue and UV Excitation}},
author = {Théodore C L Michielin and Juan J Romero and Lison Royer and Viola C D’mello and Manuel J Llansola-Portoles and Damien R Sorigué and Pavel Müller},
journal = {ACS Catalysis},
year = {2026},
month = jul,
doi = {10.1021/acscatal.6c04891},
url = {https://hal.science/hal-05775378v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Different Photons, Same Chemistry: Identical Mechanism and Efficiency of Fatty Acid Photodecarboxylase under Blue and UV Excitation
AU - Théodore C L Michielin
AU - Juan J Romero
AU - Lison Royer
AU - Viola C D’mello
AU - Manuel J Llansola-Portoles
AU - Damien R Sorigué
AU - Pavel Müller
PY - 2026
DA - 2026/07/30
JO - ACS Catalysis
DO - 10.1021/acscatal.6c04891
UR - https://hal.science/hal-05775378v1
AB - Fatty acid photodecarboxylase (FAP) is a promising photoenzyme for light-driven hydrocarbon synthesis and a versatile chassis for photobiocatalysis. A recent study proposed that UV/violet illumination enhances FAP activity relative to blue light by accessing a distinct, more efficient photochemical regime. Here, steady-state and time-resolved fluorescence, transient absorption spectroscopy under single-turnover conditions, and product quantification under continuous irradiation show that the quantum yield of decarboxylation, the identities and kinetics of reaction intermediates, and the overall chemical yield are identical for blue and UV/violet excitation. These findings establish that FAP operates through a single, wavelength-independent photocycle, overturning the proposed "violet-enhanced" reactivity model.
SN - 2155-5435
SN - 2155-5435
ER -