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Peer-reviewedCC BY 4.0PLOS One

Heterotrimeric G proteins containing Gβ₁γ₂ subunits exhibit subtype-specific mobility differences in live cells

  • Ondřej Kuchynka1
  • Andrei Kovalchuk2
  • Mia Nußbaumer3
  • Ekaterina Sviridova
  • Tomáš Fessl
  • Alexey Bondar
  1. 1Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czechia
  2. 2Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czechia
  3. 3Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czechia
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Abstract

Heterotrimeric G proteins are key signal transducers in all eukaryotic cells. Anchored to the inner leaflet of the plasma membrane, they transmit and amplify extracellular chemical and physical signals downstream of G protein-coupled receptors. Despite numerous available studies, many biophysical aspects that regulate G protein signaling, including membrane mobility, remain poorly understood. Here, using single-molecule imaging, we show that different subtypes of heterotrimeric G proteins containing Gβ1γ2 subunits exhibit high diversity in their membrane mobility. We demonstrate that the nature of the Gα subunit plays a major role in defining the mobility of such heterotrimers. Our results indicate that heterotrimers containing Gα12 and Gα13 subunits exhibit markedly reduced mobility compared to those containing Gαi/o and Gαs subunits. These findings identify subtype-specific lateral membrane mobility of G proteins as a factor that can affect their signaling dynamics in living cells.

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Cite

BibTeXRIS
BibTeX
@article{kuchynka2026heterotrimeric,
  title = {{Heterotrimeric G proteins containing Gβ₁γ₂ subunits exhibit subtype-specific mobility differences in live cells}},
  author = {Ondřej Kuchynka and Andrei Kovalchuk and Mia Nußbaumer and Ekaterina Sviridova and Tomáš Fessl and Alexey Bondar},
  journal = {PLOS One},
  year = {2026},
  month = sep,
  doi = {10.1371/journal.pone.0359304},
  url = {https://doi.org/10.1371/journal.pone.0359304},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Heterotrimeric G proteins containing Gβ₁γ₂ subunits exhibit subtype-specific mobility differences in live cells
AU  - Ondřej Kuchynka
AU  - Andrei Kovalchuk
AU  - Mia Nußbaumer
AU  - Ekaterina Sviridova
AU  - Tomáš Fessl
AU  - Alexey Bondar
PY  - 2026
DA  - 2026/09/29
JO  - PLOS One
DO  - 10.1371/journal.pone.0359304
UR  - https://doi.org/10.1371/journal.pone.0359304
AB  - Heterotrimeric G proteins are key signal transducers in all eukaryotic cells. Anchored to the inner leaflet of the plasma membrane, they transmit and amplify extracellular chemical and physical signals downstream of G protein-coupled receptors. Despite numerous available studies, many biophysical aspects that regulate G protein signaling, including membrane mobility, remain poorly understood. Here, using single-molecule imaging, we show that different subtypes of heterotrimeric G proteins containing Gβ1γ2 subunits exhibit high diversity in their membrane mobility. We demonstrate that the nature of the Gα subunit plays a major role in defining the mobility of such heterotrimers. Our results indicate that heterotrimers containing Gα12 and Gα13 subunits exhibit markedly reduced mobility compared to those containing Gαi/o and Gαs subunits. These findings identify subtype-specific lateral membrane mobility of G proteins as a factor that can affect their signaling dynamics in living cells.
SN  - 1932-6203
ER  -